RESEARCH DESCRIPTION: Research in different areas related to A) Infection prevention, including hand hygiene compliance in healthcare workers and surveillance of hospital acquired infections through chart studies, B) Mentorship: Needs of students for mentorship will be assessed and the information will be used to guide planning, implementation, evaluation, and decision making to develop mentorship programs related to the field of healthcare.
Projects will be conducted at the UCI Medcial Center.
REQUIREMENTS TO PARTICIPATE: Students must complete requirements for human subject research and Biosafety 194 and Research Ethics course and have strong analytical and social skills. Familiarity with the Excel program will be helpful.
COURSE COMPLETION: Students must be in their junior or senior years.
GRADE POINT AVERAGE: Minimum GPA of 3.0 is required.
TIME COMMITMENT PER WEEK: 3-4 hours per week is required but the hours can be spread throughout the week. Students can continue the project throughout the year and get units each quarter.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Commitment and enthusiasm in finishing assignments and helping with data analysis. OFFICE LOCATION: UCIMC, 101 City Drive, Bldg 58, Orange, CA 92868
The focus of my lab is to understand the biology of dendritic cells (DC) of the immune system. DCs are the major antigen presenting cells that are critical mediators of both innate and adaptive immune responses. We are mainly interested in understanding the differences in the functioning of DCs between young and aged human subjects and the consequences and mechanisms underlying these differences. We study the activation of DCs by Toll like receptors (TLRs) ligands by Flow cytometry and ELISA. Signaling differences are studies by Western and flow cytometry. The priming of T cells by DCs forms a major component of our adaptive immune system studies.
Office location: Med Sci I, C-238
Should work for at least 1 school yr. GPA: 3.5+. 12-15 hrs/wk.
Contact:
Thomas Ahlering, M.D.Urology
RESEARCH DESCRIPTION:
Dr. Ahlering’s research focuses on the diagnosis, treatment, and clinical outcomes of prostate cancer. Dr. Ahlering is continuously trying to find new methods of faster return to continence and potency post radical prostatectomy. The Bio199 student will be involved in clinical research projects by helping to carry out studies, collecting data, maintaining study databases and analyzing data. He/She will be expected to meet with Dr. Ahlering and his research team on a weekly or biweekly basis to give updates on the clinical outcomes and findings of the data analysis. The student may be a co-author on manuscripts depending on the level and quality of work and analysis produced during his/her time as part of the research team.
REQUIREMENTS TO PARTICIPATE: Major in the Biology Department
At least a third year undergraduate
COURSE COMPLETION: To be determined
GRADE POINT AVERAGE: Minimum 3.0 GPA
TIME COMMITMENT PER WEEK: Two to three days a week, about 10 hours.
NUMBER OF UNITS REQUIRED: 2 - 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Attendance, database upkeep, and quality of work. OFFICE LOCATION: UCI Medical Center, City Tower
333 City Blvd West, Ste 2100
Orange, CA 92868
RESEARCH DESCRIPTION:
My lab works at the interface between microbiology, ecosystem ecology, and global change. We study how microbes “make a living” in the environment, and how they affect ecosystem processes, such as the break-down of dead plant material and the cycling of nutrients. Student projects may focus on field work, laboratory studies, and/or computer modeling. Summer and academic year positions are available. REQUIREMENTS: Students should be prepared to develop independent projects and spend at least 10 hours per week on research. A multi-quarter commitment is strongly preferred, and students will be expected to participate in lab activities and meetings. MEANS OF EVALUATION: grades will be based on time committed to the project and the quality of research. OFFICE LOCATION: 307 Steinhaus
RESEARCH DESCRIPTION:
Hoda Anton-Culver, PhD.focuses her research in the area of genetic epidemiology of cancer. One study funded by the NCI as an R01 combines techniques in genetic epidemiology and molecular genetics to define and characterize inherited breast/ovarian cancer in the population and in the familial component of the disease. This study began in the late 1980’s and continues to date through competing renewals of the NCI funding. Another NCI funded R01, focuses on colon and rectum cancers to study the effects of genetic influence on the mismatch repair genes as they interact with diet, physical activity, and other factors that may influence the risk for colorectal cancer and estimate genetic and environmental interaction. In addition to breast and colorectal cancer she has funding to investigate genetic and environmental influences on the risk of multiple other cancers including prostate, malignant melanoma and childhood cancer. She has been awarded several significant awards in cancer genetics from the National Cancer Institute. These awards include the (1) "UCI-UCSD Cancer Genetics Network" to collaborate with other centers to investigate the genetic basis of human cancer susceptibility and identify means to address the public health issues associated with human cancer genetics; (2) The "NCI Cancer Genetics Network Informatics Center at UCI" supports the cooperative of Cancer Genetics Networks as the sole Informatics Center responsible for the design, implementation, and maintenance of an information management system that supports the Network-wide research protocols
REQUIREMENTS TO PARTICIPATE: Biology, Genetics, Statistics, and Epidemiology
COURSE COMPLETION: at least 2 courses of the above are required
GRADE POINT AVERAGE: GPA 3.0 minimum
TIME COMMITMENT PER WEEK: 8- 12 hours
NUMBER OF UNITS REQUIRED: 2-3. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Practical, oral and written evaluation. OFFICE LOCATION: 224 Irvine Hall, Zot 7550
Tallie Z. Baram, M.D., Ph.D.Anatomy/Neurobiology;Pediatrics
Neuroscience Research. Cellular mechanisms of vulnerability of the developing brain to febrile and other seizures. Mechanisms of excitation-dependent neuroplasticity. Molecular neurobiological mechanisms of response to stress during development.
Lab or building where research will be conducted:
Field Work in Orange County and Long Beach with Community Health Organizations. CURRENT RESEARCH INTERESTS AND DESCRIPTION:
Conducting Research to prevent chronic disease (cardiovascular, diabetes, obesity and build bone health) through lifestyle interventions in minority communities through school and community programs. Research Studies currently involve preventing type 2 diabetes with middle school children and their families as well as the establishment of network for communicating research findings to the diverse population of Orange County
PREREQUISITES: Upper division students who have completed Bio 194 and ethics course Completion of all human subject tutorials and prerequisites, biology or statistical background (with introductory courses in statistics) highly recommended. Prior work or volunteer experience working with diverse communities. Strong English written and verbal skills necessary. Grade Point average of 3.0 or higher
SELECTION CRITERA: Resume, experience in and /or interest in community health, writing experience, and social skills to work in diverse communities. Upper Division standing. Interview will be required. Please submit resume of course work, volunteer experience and number of hours available per week via e-mail to slbassin@uci.edu
UNIT REQUIREMENT:
Number of Units/Quarter 2 or more units/per quarter (4 hours per unit)
Hours/Week 8 hrs per week
GRADING BASIS:
Examination: Initiative and research paper to be submitted and /or develop a proposal for funding with a community organization.
UCI faculty member will evaluate quantity of Work Research paper and / or funding proposal for content and exposition
RESEARCH DESCRIPTION: There is a high incidence of arrhythmias in patients undergoing the Fontan surgery for palliation of single ventricle hearts. The incidence of these arrhythmias using two different surgical techniques is unclear. The purpose of this research is to compare the incidence of arrhythmias between these 2 different surgical techniques (lateral tunnel vs. extracardiac conduit).
REQUIREMENTS TO PARTICIPATE: Understanding of human cardiac anatomy.
TIME COMMITMENT PER WEEK: 10 hours
NUMBER OF UNITS REQUIRED: 3
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Completion of an abstract. OFFICE LOCATION: 101 The City Dr.
The projects examine the effects of nicotine in adolescent and adult rats. Will learn to perform survival surgery on rats, and intend to work in the lab for 1 – 2 years. The work requires daily animal handling, sterile preparation of drugs, and careful performance of the experiments. Accepts students that have GPA greater than 3.0, can work in the lab 12 – 16 hours per week spread over three days.
Lbachir BenMohamed, Ph.D.Ophthalmology and Center for Immunology
Lbachir BenMohamed, Ph.D. and Anthony B. Nesburn, M.D.
OFFICE LOCATION: UCIMC, Building 55, 2nd Floor, Room 202
RESEARCH DESCRIPTION:
Immunology of Herpes Simplex Virus (HSV) Type 1 Latency and Pathogenesis
The aim of this study is listed in three sections:
1) To develop a clinically acceptable vaccine strategy to reduce or eliminate ocular and genital herpes infection including recurrent disease.
2) Eventually, the candidate vaccines developed in mice will be tested in rabbits and humans. Techniques used in this project: Immunology, virology, and molecular biology - techniques that include vaccine studies in animal models.
3) To investigate if (1) LAT is involved in dendritic cells (DC) modulation (2) HSV-1 interferes with the migratory activity of DC in vivo (trafficking or homing), (3) LAT also plays a role in this viral activity.
Development of immunotherapeutic and immunoprophylactic strategies against various cancers
1) The aim of this study is to develop a clinically acceptable Glyco lipopeptide vaccines to reduce or eliminate tumor cells
2) The candidate vaccines developed will be tested in mouse model of cancers. Techniques used in this project: Immunology, virology, and molecular biology - techniques that include vaccine studies in animal models.
REQUIREMENTS TO PARTICIPATE:
Requirements - General background in biology/biochemistry/immunology/virology, oncology and/or infectious diseases.
Strong motivation, good organizational skills, quick study and receptive to taking work direction.
COURSE COMPLETION: Completion of core through Bio 99 with a grade of B or higher, completion of Bio 194S Safety, consent of instructor.
GRADE POINT AVERAGE: GPA 3.0 or higher
TIME COMMITMENT PER WEEK: Minimum 25 hours per week.
NUMBER OF UNITS REQUIRED: 4
OTHER: Accepts students that can work in the lab 25 hours per week spread over three days.
Interested in students who will make at least a multi-quarter commitment (one to two years preferred).
Currently six (6) positions available and routinely students needed on an on-going basis.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of Work and Weekly Meeting.
This research is takes place at UCIMC under the direct supervision of Dr. Bera. Students will join on-going clinical trials of both inpatients and outpatients who are enrolled in clinical research programs. These trials include medication evaluations, neuropsychological testing and retrospective medical record reviews. Students will be trained in psychiatric evaluation of multiple disease states and have the opportunity to work directly with 3rd and 4th year medical students and psychiatry residents with patients who are hospitalized. Students will get state of the art clinical contact with patients that will help them in future clinical opportunities. Independent research ideas to be studied will be encouraged. Presentation at scientific meetings along with submission of findings to scientific journals will be encouraged.REQUIREMENTS TO PARTICIPATE: Motivation and interest in direct patient contact in both clinical and research setting.
COURSE COMPLETION: Minimum of 6 month committment
GRADE POINT AVERAGE: Minimum GPA of 3.00
TIME COMMITMENT PER WEEK: 6 to 10 hours/week
NUMBER OF UNITS REQUIRED: 3 to 4 units
OTHER:
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm of effort and completion of assigned work.
RESEARCH DESCRIPTION: Induction of cervical carcinoma and other types of cancers by papillomaviruses. Specifically regulation of papillomavirus transcription during the normal life cycle and during carcinogenesis.
REQUIREMENTS TO PARTICIPATE: Preferentially 3rd/4th year student, knowledge about transcription, virology, cell biology.
COURSE COMPLETION: n/a
GRADE POINT AVERAGE: n/a
TIME COMMITMENT PER WEEK: minimum of 8 hours (1 full day or 2 half days) plus participation in group seminar (normally Wednesday at noon), preferentially for 2 or 3 quarters.
NUMBER OF UNITS REQUIRED: n/a
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: enthusiasm and regular completion of committed time. OFFICE LOCATION: 114 Sprague Hall
COURSE TITLE: Role of Astrocytes in Epilepsy.
OFFICE LOCATION: Med Sci 1, Room C356
RESEARCH DESCRIPTION:
Astrocytes, the most abundant glial cell type in the brain, are involved in many important physiologic functions such as neurotransmitter, potassium, and energy metabolism. Accumulating evidence over the past few years suggests that astrocytes may also powerfully modulate brain excitability. Therefore, neuroscientists have begun to investigate the hypothesis that glial cells may contribute to the hyperexcitability of epilepsy. Indeed, pathologic specimens from patients with temporal lobe epilepsy often demonstrate marked reactive gliosis (glial cell scarring), and alterations in distinct astrocyte membrane channels, receptors and transporters have all been associated with various forms of epilepsy.
Our recent research has focused on the study of the astrocyte water channel aquaporin-4. Distinct aquaporin “water channels” have been shown to be important for water transport in many different tissues. In the brain, aquaporin-4 is involved in water and ion metabolism. Recent studies have shown powerful alterations in excitability, seizure duration, extracellular space and potassium regulation in aquaporin-4-deficient mice. These results coincide with results demonstrating altered levels of aquaporin-4 in human temporal lobe epilepsy. However, it remains to be determined how the cellular expression and function of aquaporin-4 is altered during the development of epilepsy. We will be examining these and related questions in well-established animal models of epilepsy. The goal of this research is to further understand astrocyte control of water and potassium metabolism in the brain, and how it goes awry in the development of epilepsy. This could directly lead to new concepts and targets for anticonvulsant drug development that may have many fewer side effects than current therapies.
Students will be directly involved in learning and contributing to the laboratory through the following techniques:
Small animal anesthesia and stereotactic neurosurgery
In vivo electrophysiology and pharmacology
EEG recording and analysis
Fluorescence imaging
Histology
Immunohistochemistry
Confocal microscopy
In situ hybridization
Western blotting
PCR
REQUIREMENTS TO PARTICIPATE: Must be highly motivated and interested in neurosciences, particularly epilepsy and in vivo imaging. Successful performance may lead to a career in clinical/translational neuroscience, neurology, and/or neurological surgery. Prior laboratory experience desired. Introductory biology course work required. Prior neurobiology course work desired.
TIME COMMITMENT PER WEEK: 10-15 hours, flexible work schedule including some weekends.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm and commitment for development of laboratory skills, research methods, and scientific acumen. Students will be involved not only in generating laboratory data, but also in literature reviews and laboratory presentations. All of these will contribute towards both their education and evaluations.
Our research focuses on the role of nuclear hormone receptors in embryonic development, adult physiology and human diseases such as obesity, cancer, inflammation and aging. Nuclear hormone receptors are ligand modulated transcription factors that directly regulate the expression of target genes. Students will gain experience in a broad range of research techniques including molecular biology, biochemistry, developmental biology, cell biology, cancer biology, molecular endocrinology, and genomics. Students beginning in summer and continuing through the year will be given preference, however, starting at any time in the academic year is possible for outstanding students if space is available. Students can visit the laboratory web site for more information. Generally speaking, completion of the first 2 years of the Bio Sci core is required. Exceptions have occasionally been made in the past for highly qualified and motivated students. Bio 103 and/or 104 are often helpful but not required. A GPA of 3.0 or higher is required. The nature of our research requires a time commitment of at least 20 hours per week for a minimum of one year. The possibility of a second year is advantageous as this will allow the student to undertake an independent research project. Although it is not required, students in the lab are strongly encouraged to participate in the Excellence in Research Program, the University Research Opportunity Program (UROP) and the Summer Undergraduate Research Program (SURP). Previous students have published in major scientific journals and in the UCI Undergraduate Research Journal. Please contact Dr. Blumberg directly if you are interested.
The overall project goals are to understand how aging changes gene expression in the brain and how these changes may help explain the decline in neuroimmunological function associated with aging. We use lipopolysaccharide (LPS, a toxic, inflammatory stimulus of innate immunity) dosing, and exposure to a novel environment (behavior), as modulators the assess brain function in old mice compared to young. The project will determine the degree to which old mice express these genes differently than young, and how the expression pattern of the response to LPS differs in old mice. Expression changes of one gene, known to occur when animals are exposed to a novel environment, may also differ in the old mice that have also been challenged with LPS. The occurrence of another gene, previously undetected in brain and of unknown function there, will be confirmed, and its response to age, inflammatory challenge, and novel environment will be determined.
The 199 Research will consist of extraction and purification of total RNA from previously-frozen mouse brains. Then expression levels of individual gene transcripts in the RNA samples will be measured by real time (quantitative) RT-PCR. A final aspect of the project is to summarize and prepare the results for poster presentation or publication.
RESEARCH DESCRIPTION:
1. Translational Research: There is an unmet clinical need for the therapy of malignant gliomas with a median survival of <12 months despite available treatments. Traditional chemotherapy relays on DNA damage and disruption of mitotic machinery, with limited effect in prolonging patient survival. The focus of my research is to test novel agents, in an attempt to target downstream molecular mechanisms involved in tumor growth and resistance to apoptosis. The final goal is to provide scientific bases for clinical trials in patients with newly-diagnoses and recurrent malignant gliomas.
2. Clinical and Quality of Life Research:
The main purpose of this study is to conduct a retrospective and prospective chart review to examine associations between care plan timelines and demographic variables in a large clinic sample of patients with brain tumors. Our research aims are to (1) compute correlations among the different variables and determine the average delay of care for Spanish speaking only patients versus native English speaking patients and (2) to test if a patient navigator can improve the access to care for brain tumor patients resulting in a decrease in time from diagnosis to treatment.
REQUIREMENTS TO PARTICIPATE: Motivation and interest in conducting research in both clinical and laboratory setting. Prior laboratory experience desired for the translational research.
COURSE COMPLETION: Minimum of 6 month commitment
GRADE POINT AVERAGE: 3.4
TIME COMMITMENT PER WEEK: 15-20 hours/week
NUMBER OF UNITS REQUIRED: 3 to 4 units
OTHER: Would prefer at least 15-20 hours/week over 3 days every week.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Research presentation at the end of rotation, participation in lab meetings, commitment and professionalism. OFFICE LOCATION: UC Irvine Medical Center, Bldg 55, Room 121
Adriana Briscoe, Ph.D.Ecology and Evolutionary Biology
RESEARCH DESCRIPTION:
Research positions are available to work on a number of projects related to studying the evolution and functional characterization of opsin genes in butterflies. Students will have the opportunity to learn molecular biological techniques such as PCR, cloning and sequencing, and after such techniques are mastered, will be encouraged to develop an independent project in their second year. Minimum requirements are at least a 1 year commitment, 3 units (9 hours/week) per quarter, mandatory lab meeting attendance and a G.P.A. of 3.3+.
GRADE POINT AVERAGE: 3.3+
TIME COMMITMENT PER WEEK: 9-12 hours/week
NUMBER OF UNITS REQUIRED: 3
OTHER: Mandatory lab attendance, written summary of research progress at the end of each quarter.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Successful acquisition of experimental data and lab meeting attendance. OFFICE LOCATION: MH5248
RESEARCH DESCRIPTION:
Primary open angle glaucoma (POAG) represents a serious and growing health problem accounting for ~12% of global blindness. Studies have identified age, ancestral group (racial background), and intra-ocular pressure (IOP) as significant risk factors for the development and progression of POAG. The objective of our research is to define the 3 dimensional biomechanical properties of the optic nerve head (ONH ) by microscopically reconstructing the structural components and biomechanical properties and relate these properties to POAG risk factors.
In our lab, state of the art technologies to globally assess the three dimensional structure and mechanical properties of the ONH are used. These technologies utilize non-linear optical affects that occur when photons generated by ultrafast lasers interact with tissue and include: 1) two photon excited fluorescence (TPEF), and 2) second harmonic generation (SHG). SHG and TPEF allow us to selectively and specifically visualize and quantify collagen (SHG) and elastic fibers (TPEF) within the 3D space of unfixed and unstained ONH tissue. This allows direct study of the effects of pressure on the structural organization of the ONH and LC. Additionally, semi-automated array tomography is used to 3 dimensionally reconstruct the entire ONH at high resolution and volumetrically measure the collagen and elastin distribution and content within the LC.
REQUIREMENTS TO PARTICIPATE:
General biology, physics, and a willingness to learn
COURSE COMPLETION:
GRADE POINT AVERAGE:
3.0
TIME COMMITMENT PER WEEK:
minimum 4 hours
NUMBER OF UNITS REQUIRED:
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Laboratory notebook, participation in lab meetings/discussions, participation in UROP undergraduate research day. OFFICE LOCATION: Orange Campus, Bldg 55, room 208
Project 1). Asymmetric Division of Neural Stem Cells. Study the mechanisms of cell division in mammalian neural stem cells, using a rat model of Parkinson’s Disease or stroke. Test the hypothesis that these cells undergo asymmetric cell division, by immunostaining and confocal microscopy using antibodies against the human homologs of asymmetric cell division determinants identified genetically in Drosophila.
REQUIREMENTS TO PARTICIPATE:
• Interest in stem cell biology
• 2-page proposal during the first two weeks
• 5-page report due at the end of Finals Week.
• 4 units of work (12 hours/wk). 3.5 GPA.
Project 2). Monitoring Local Biodiversity. Work with the faculty advisor and other local experts to document and monitor local wildlife populations, choosing from one of the following:
• Photo-documentation of local species of invertebrates (mainly insects and spiders), field studies of their distribution, seasonality and abundance, and contribution to a web site showing the life history, interesting features, and conservation issues concerning each species.
• Bird counts at Upper Newport Bay and/or the San Joaquin Wildlife Sanctuary
• Marine Life Inventory at Upper Newport Bay
REQUIREMENTS TO PARTICIPATE:
• Some knowledge of or at least interest in local natural history.
• Willing to do unsupervised field work after training is completed.
• Field work may require the student to obtain or rent equipment (binoculars, spotting scope, camera) and obtain a field guide.
• 2-page proposal during the first two weeks
• 5-page report due at the end of Finals Week.
• 4 units of work (12 hours/wk). 3.5 GPA.
Project 3). Campus Sustainability. Investigate the operation of the UCI campus to identify activities that can be made more independent and sustainable, and initiate student action to make improvements. Projects include use and reuse of energy and water, use of drought-resistant native plants, minimizing waste, use of biodegradable materials in food facilities, general recycling and waste reduction projects, and pollution-free transport. Methods include library and internet research, interviews with campus decision-makers, and comparison with other campuses followed by practical participation and promotional activities for improvements through student organizations. Projects will be supervised by the faculty advisor and the UCI Recycling Coordinator.
REQUIREMENTS TO PARTICIPATE:
• Willingness to carry out research as well as practical work at the campus recycling center.
• 2-page proposal during the first two weeks
• 5-page report due at the end of Finals Week.
• 4 units of work (12 hours/wk). 3.5 GPA.
William Bunney, M.D.Psychiatry and Human Behavior,
TWO PROJECTS: Project #1 -- Contact Julie Patterson, Ph.D., at (714) 456-1663 or jvpatter@uci.edu -- UCI Medical Center - This research is using brain function (electroencephalogram or EEG), neuropsychological (tests of memory, attention, reaction time, etc.), symptom, and clinical interview measures to study the contribution of brain inhibitory mechanisms and information processing deficits to the symptoms of schizophrenia and bipolar disorder (manic depression). The students will have the opportunity to have direct patient contact by helping with patient interviews and testing. The research is done at the UCI Medical Center. Students are needed on an ongoing basis.--------------------------------------------- Project #2 -- Contact David Walsh, PsyD, ABPP, at (949) 824-5013, RESEARCH DESCRIPTION: UCI Main Campus - This project is funded by the National Institute of Mental Health to examine genetic, architectural, and biochemical brain abnormalities in major depression, bipolar affective disorder and schizophrenia. This opportunity would be appropriate for students interested in research of severe mental illnesses. Students would develop a clear understanding of DSM-IV diagnostic criteria, risk factors for suicide and general research methodology. Students are needed on an ongoing basis. REQUIREMENTS TO PARTICIPATE: Motivation and interest in clinical research. GRADE POINT AVERAGE: Minimum GPA of 3.00 TIME COMMITMENT PER WEEK: 6 to 10 hours per week FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm of effort and completion of assigned work. OFFICE LOCATION: Main Campus – 5251 California Ave, Suite 160, Irvine, CA
REQUIREMENTS TO PARTICIPATE: Interest in clinical research.
TIME COMMITMENT PER WEEK: A minimum of 6 hours per week (2 units) is preferred.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality and quantity of work.
Contact:
Jorge Busciglio, Ph.D.Neurobiology
This course is directed to provide students with an overall view of laboratory techniques to evaluate neuronal survival and degeneration, and to review the role of neuronal loss in neurological diseases.
Vincent Caiozzo, Ph.D.Physiology & Orthopedic Surgery
Sophomore standing or higher. Studying hibernating muscle physiology & disuse atrophy in several non-model species, including ground-squirrels, bears, bats and marmots. Examining lizard species as well. Utilization of several molecular biology techniques. Involves analyzing the protein and mRNA profiles of myosin in muscle, using RT-PCR and SDS-PAGE. Cloning myosin genes in the above species. Summer work may involve some travel to collect samples from collaborating institutions ( Colorado and the Sierra Nevada , CA).
Anne L. Calof, Ph.D.Anatomy & Neurobiology, Developmental & Cell Biology
199 students are needed to participate in ongoing research on neural stem cells and molecular signals that regulate development and regeneration of the nervous system. Successful 199 students from the laboratory frequently become authors on papers published in scientific journals, and may have the opportunity to present their research at national conferences. Requirements : Minimum Sophomore (for exceptional cases) or Junior standing, 3.4 GPA (this is sometimes negotiable), Minimum commitment 1 year (4 quarters), 2 years preferred, willingness to perform 1 st quarter Pass/no pass basis to learn lab techniques, must spend 8-12 hours per week, attend weekly lab meetings and present research in lab meetings.
Research in my lab concern the mechanisms of evolution in natural plant populations. Current projects focus on plant hybrid zones. We are comparing the fitness of plant hybrids to that of the parental species to test particular models for genetic differentiation and evolution of new species. Approaches include experimental field studies in the Colorado Rockies and the eastern Sierras. These involve measurement of insect and bird pollinator behavior, pollination success, photosynthetic and other physiological traits, and seed production in common gardens. At UCI we do greenhouse studies of floral and physiological traits, and DNA analyses to examine the genetic structure of natural hybrid zones. Students with strong interest in ecology, genetics, and/or evolution. Bio 93 and 94, or Bio 96 required. Bio 97 and E106 are helpful. Minimum 3.2 GPA. 6-12 hours/ wk. 2-4 units required.
Sophomore standing and above. Completion of Bio. 94. I am investigating methods to reforest eroded, nutrient-deficient, toxic tropical soils where rainforest was cut for pasture 50 years ago, and the land severely overgrazed. We have many long term experiments in Costa Rica on regeneration of forest and of topsoils. Students may participate in my 199 program either by analyzing one of these ongoing experiments or by establishing their own experiment in the field in Costa Rica . Examples of potential topics include the role of soil biology and chemistry in tree growth, the characteristics of trees capable of growing in eroded soil, and the repopulation of reforested areas by birds and insects. Two quarters of preparatory research are required if a student wishes to work with us in Costa Rica in the summer. Details of our various research approaches are on the website
Our interest is how normal tissues contribute to the metastasis (spread through out the body) of breast and other cancers. We have identified a basement membrane protein, laminin 5 that is secreted by normal breast cells, yet causes increased migration and spread of breast cancer cells. We are developing inhibitors to this process that might aid in patient treatment. 1 year biology, biology lab and general chemistry, at least 1 quarter of organic chemistry. Recommendation from your lab instructor is required. 12 hours minimum, 15-18 preferred. 3 units will be given for 12 hours work. GPA: B or better. The lab is at UCI Medical Center, so students should be prepared to commute and comply with additional UCIMC requirements.
Research in Dr. Casali's lab is at the cutting edge of modern molecular and cellular biology. It investigates the mechanisms of class switch DNA recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin genes using in vivo and in vitro models. Students will be trained in advanced molecular biological techniques including but not limited to: aseptic technique, cell culture protocols, DNA and RNA isolation, cDNA synthesis, molecular cloning, PCR, PAGE, Western and Northern blotting, EMSA, and ELISA. Work involves human lymphocytes and/or transgenic mice. Interested students should submit an updated resume with relevant research experience. Project assignments will be determined by the commitment and enthusiasm of the student to lab work. Interested students are encouraged to visit the lab prior to requesting a position. Minimum GPA required: 3.0. In order to be considered for a bio199 position, please send a completed
application to epone@uci.edu.
Application url: casalilab.bio.uci.edu/application.doc
Lab website: casalilab.bio.uci.edu
Lab Blog: casalilab.blogspot.com
The use of foreign antibodies for the treatment of several diseases in humans
The use of specific antibodies to treat disease is of growing importance, especially for the treatment of cancer, neurological diseases, and infectious diseases. Unfortunately, the source of these antibodies is very limited since the patient has to be treated with the same antibody several times. Antibodies produced in animals are limited to a single use, as subsequent administration of the same antibodies will induce a harmful immune respone (e.g. allergic reaction) and can lead even to death. Additonal antibodies derived from animals is viewed as foreign by the human body and can elicit an antibody reaction on its own and be eliminated before having an effect. Methods for rendering these proteins safe for multiple injections and keep the antibodies functionell are being actively researched including production of hybrid monoclonal antibodies and the "humanized" animals. However, many of these methods are time consuming , expensive and complicated, and mostly specific for only one disease.
We developed a new technology to make these proteins from animals safe for multiple injection, and this methode is less time consuming, less expensive and not very complicated, and it is not disease specific. Currently we want to evaluate our new technique to determine if we are able to use these antibodies for treating diseases such as infectious diseases and autoimmune diseases.
Therefore, we are looking for students who are interested in immunology and infectious diseases. The students will be involved in immunological techniques such as ELISA and Westernblot and molecular biological techniques such as cloning, polymerase chain reaction , and tissue culture and also in handling the animals.
Recent studies from a private Laboratory (PlasVacc USA, Templeton, CA), however, suggested that mice will tolerate multiple injections of a modified technique to produce cryoprecipitate-depleted plasma (Cryoprecipitate is a precipitate that forms when normal blood plasma is cooled, and is especially rich on clotting factors-the remaining plasma contains the antibodies). The plasma obtained with this improved technique is now termed cryoprecipitate-FREE plasma. THE DIFFERENCE BETWEEN CRYOPRECIPITATE-FREE PLASMA OR DEPLETED PLASMA IS THE AMOUNT OF THE CRYOPRECIPITATE ASSOCIATE PROTEINS. CRYOPRECIPITATE CONTAINS TYPICALLY 40% OF THE PROTEINS PRESENT IN THE PLASMA; THE REMAINING 60% ARE STILL IN THE PLASMA WHICH IS TERMED CRYOPRECIPITATE-DEPLETED PLASMA. THE CRYOPRECIPITATE-FREE PLASMA IS FREE OF CRYOPRECIPITATE ASSOCIATE PROTEINS. The mice survived three immunization with the cryoprecipitate-FREE plasma after the plasma was sterilized over a column (personal communication-see attachment). However, this company never tested, if the antibodies are still functional. Therefore, we wish to perform a study to test the hypothesis that cryoprecipitate-free plasma is 1) tolerated by animals from a different species by repeated injections and 2) the antibodies in this plasma are still functional. We will use rabbits as the plasma donors, and mice as the test subjects.
Jeff Chan, Ph.D.Pathology, Biological Chemistry, Toxicology
Research areas: Cell growth and function require the coordinate regulation of gene expression by transcription factos. My lab seeks to understand the role of CNC-bZIP transcription factors in oxidative stress response. Diverse molecular and genetic approaches including trasngenic and knockout mouse models, DNA-microarrays, cell culture, and yeast are being applied to delineate the physiological roles of CNC factors in cell function; their role in oxidative stress responses and the genesis of cancer; and the mechanisms by which these factors act. Biology & chemistry courses. 3 units. At least 12 hours of work. GPA 3.0 or better.
The brain is constantly bombarded with sensory input from the environment and must receive, process and respond to this information. The research in this laboratory seeks to understand how neurons in the cortex process information. We study this question by recording from individual living neurons in slices of brain tissue, and also by building computational models of neurons and networks of neurons. For a description of current projects, please see the listed website. Http://chancelab.bio.uci.edu For projects involving electrophysiology, the student will stimulate and record from neurons in slices of rodent brain tissue. For projects involving computational modeling, the student will build and study computational neural networks (using C, Matlab or another language mutually agreed upon by instructor and student). Prerequisites : Consent of the instructor. Ideally, student will have completed Bio 110. Student must be willing to commit for at least two consecutive terms, preferably longer. 4-5 units/quarter. GPA 3.2. Knowledge of a programming language would be extremely beneficial.
Our lab studies the relationship between specialized membrane proteins, potassium channels, and disease. One of the channels we are interested in, Kv1.3, is a voltage gated channel which is upregulated in fully differentiated autoreactive T-cells. We are currently developing channel blockers for Kv1.3 as therapeutics for autoimmune disease (multiple sclerosis, type I diabetes, and rheumatoid arthritis). The second potassium channel that we study, the SK channel, is implicated in several neurological disorders and also hypertension. We have generated a transgenic mouse in which this channel's function has been silenced in dopaminergic neurons. We believe that this mouse has the potential to act as a model for ADHD (attention deficit hyperactivity disorder), drug addiction, and also hypertension. We are looking for responsible students with a GPA of at least 3.0 to carry out work in one of these areas. All of our available projects have direct clinical implications, and students will gain experience in understanding how a single class of molecules can lead to disease. We have had previous 199 students that have gone on to medical schools and top graduate programs in the biological sciences.
RESEARCH DESCRIPTION:
Cellular and molecular mechanisms underlying hormone and growth factor regulation of vasodilatation and angiogenesis at the maternal, fetal and placental interface with focuses on reactive nitrogen and oxygen species.
REQUIREMENTS TO PARTICIPATE: Sophomore and up
COURSE COMPLETION: N/A
GRADE POINT AVERAGE: GPA >3.5
TIME COMMITMENT PER WEEK: 12-16
NUMBER OF UNITS REQUIRED: 3-4
OTHER: Basic lab safety trainings
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
1. Attendance
2. Experimental lab notes
3. Lab meeting presentations (2-3 times per q quarter). OFFICE LOCATION: Med Surge I, Build 810, Rm 140
RESEARCH DESCRIPTION:
The central theme of my research is to elucidate the fundamental mechanisms governing genome integrity with specific emphasis on the intracellular signaling pathways involved in the DNA damage response and repair. Currently we focus on dissecting DNA surveillance mechanisms. Diverse molecular and genetic approaches using mammalian cell culture and yeast are being applied to delineate the DNA checkpoint factors in cell function; their role in DNA damage responses and repair.
COURSE COMPLETION: Cell Biology, Genetics, Molecular Biology, Biochemistry,
GRADE POINT AVERAGE: GPA 3.0 or higher
TIME COMMITMENT PER WEEK: at least 12 hrs of work
NUMBER OF UNITS REQUIRED: at least 3 units
OTHER: one-year commitment.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Students will be graded based on their performance and given a numeric number of A through F. LOCATION OF RESEARCH: Med Sci 1, Room D268
Bernard Choi, Ph.D.Biomedical Engineering and Surgery, (Beckman Laser Institute)
RESEARCH DESCRIPTION:
The mission of the Choi Microvascular Therapeutics & Imaging (MTI) Lab is to develop instrumentation and employ methods to improve in vivo characterization of the microvasculature. 199 students in my laboratory work in a multidisciplinary environment at the intersection of engineering, vascular biology, and clinical medicine. I strongly encourage independent research projects which encompass at least one (oftentimes several) of the following topics:
1. Optical imaging instrument development/characterization
2. Animal surgery
3. Clinical research
4. Light-based therapy monitoring
5. Computational modeling
The most updated list of research opportunities can be viewed at the MTI lab website:
http://choi.bli.uci.edu/
REQUIREMENTS TO PARTICIPATE:
Requirements vary by project. I am most interested in students who have at least two years to commit to research in my lab.
COURSE COMPLETION:
Course requirements vary by project.
GRADE POINT AVERAGE: >3.5 GPA is desired.
TIME COMMITMENT PER WEEK: >8 hrs/week
NUMBER OF UNITS REQUIRED: >2
OTHER:
N/A
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
I evaluate productivity and effort, and ability to integrate into the existing MTI lab framework. OFFICE LOCATION: Beckman Laser Institute
Contact:
Bernard Choi: choib@uci.edu, (949) 824-9491
Olivier Civelli, Ph.D.Pharmacology
RESEARCH DESCRIPTION:
Areas of research: molecular pharmacology, neurobiology, drug discovery. Central theme: find novel genes involved in brain disorders. Disorders currently studied: obesity, drug addiction, memory deficits, sleep disorders, stress. Students will have opportunity to participate in experiments covering all aspects of pharmacological research: molecular (cloning) and cellular (cell culture) biology, pharmacology (binding and second messenger assays) and animal behavior (if desired).
REQUIREMENTS TO PARTICIPATE: students interested in pharmacological/pharmaceutical research; commitment: 2 years or more. Preference to those with experience in biological and/or chemical techniques.
GRADE POINT AVERAGE: 3.3+
TIME COMMITMENT PER WEEK: 12-16 hours/week
NUMBER OF UNITS REQUIRED: 3
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: participation, willingness to learn, responsibility OFFICE LOCATION: 369 Med Surge II, School of Medicine
Laboratory based research investigating mechanisms of neuronal development, with particular emphasis in the use of embryological and microscopy imaging techniques. Requirements: Bio. Majors and completion of freshman year that includes 1 yr. of general chem with labs, Bio Sci 93, and Bio Sci 94.
Assist in neurobiological studies comparing normal and abnormal brain aging (both animal and human). The primary focus will be using an animal model of human brain aging. The student will be involved with experiments that use techniques such as immunocytochemistry, immunofluorescence, confocal microscopy, Western blotting and ELISAs on brain tissue. The student will compare animals treated with antioxidants to those without to see if there is a reduction in brain pathology. Parallel studies involving human brain tissue (Alzheimer's disease and normal aged controls) will also be included as the student acquires the necessary skills.
Research is on how specific connections are formed in the brain during development and plasticity. Focus is on the auditory brainstem, where much is known about the connectivity and its importance for brain function. The lab has been examining the role of a family of proteins called the Eph receptors, and their ligands, the ephrins. Lab uses immunohistochemistry, in situ hybridization, and western blots to analyze gene expression. Lab also uses a variety of gene misexpression techniques followed by axon tracing methods to test roles for these proteins. Requirements: completion of Bio110, minimum GPA of 3.0. Must be interested in lab research and motivated to complete an independent project. 3 – 5 units, 10 – 20 hours per week.
RESEARCH DESCRIPTION: Project 1: Genetics of Cortical Plasticity. Participation will prepare students for improved understanding of the human brain, how it functions, and how genes and experience affect the plasticity of these functions. Students will be involved in research examining how genetic factors influence cortical plasticity in healthy human subjects as well as clinical populations. Main duties will include assisting with the following: performance of brain mapping such as transcranial magnetic stimulation, performance of subject assessments, analysis of brain mapping data such as functional magnetic resonance imaging, analysis of electromyograms, and statistical analyses of behavioral data. Project 2: Robotic Therapy. Participation will prepare students for an improved understanding of the human brain, how it functions and the factors that influence plasticity of these functions. Students will participate in the research process of a robotic study focused on arm motor recovery after stroke. Main duties may include assisting with the following: analysis of brain mapping data from subject functional magnetic resonance imaging and transcranial magnetic stimulation, electromyogram analysis, statistical analyses of behavioral and robot data, and/or subject assessments and robot therapy.
REQUIREMENTS TO PARTICIPATE: Commitment to participate for 2 years.
COURSE COMPLETION: Bio 194S.
GRADE POINT AVERAGE: 3.0 +
TIME COMMITMENT PER WEEK: 6-10 hours per week (flexible)
NUMBER OF UNITS REQUIRED: 3+.
OTHER: Interested students should contact Brian Minton at (714) 456-2356 or bminton@uci.edu, Stephanie McHughen (Project 1) at smchughe@uci.edu, or Lucy Der-Yeghiaian (Project 2) at lderyegh@uci.edu.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Course grade based on caliber of performance and participation in lab journal club. OFFICE LOCATION: Hewitt Hall, Room 1331
David Cribbs, Neurology & Institute for Brain Aging and Dementia
My research interests are in the degenerative mechanisms that contribute to the onset and progression of Alzheimer’s disease (AD), and the development of novel therapeutic strategies to treat the disease. My laboratory is currently focused on immunotherapy (Aβ-vaccine) as an experimental approach to test the Beta-Amyloid (Aβ) Cascade Hypothesis of AD and as a potential intervention for treating patients suffering from the disease. I utilize transgenic (Tg) mouse models that overexpress human mutant forms of the Amyloid Precursor Protein (APP/Tg). These mice develop extensive AD-like Aβplaque pathology in the brain as they age. Thus, they provide a valuable experimental platform for testing hypotheses on disease mechanisms, as well as potential therapeutic and prophylactic approaches. When the APP/Tg mice are immunized with Aβ peptide, the B cell epitope of Aβ induces antibodies that bind to Aβ, which fafilitates clearance of the peptide from the brain. However, because Aβis a self-antigen it is necessary to break the immune tolerance to self, thus the current immunization protocols rely on very powerful adjuvant systems. Unfortunately, the first clinical trial on AD patients (AN1792) was halted when approximately 6% of the participants developed an adverse inflammatory response in their brains (meningoencephalitis). Speculation on putative culprits for the failure of the clinical trial include the adjuvant (QS21) used to boost the immune response in the elderly patients, and the Aβ “self” T cell epitope. QS21 induced a strongly proinflammatory T cell-mediated (Th1) immune response specific to the Aβ self T cell epitope. Additional factors that may have contributed to the adverse events in the elderly patients that received the vaccine include the level of vascular pathology, changes in blood brain barrier permeability induced by immunization, and the effects of aging on the immune response to Aβ-immunotherapy. My laboratory is developing mouse models that replicate the adverse events that occurred with the AN1792 clinical trial. These mouse models will be used to test the safety of “second generation” vaccines that incorporate alternative strategies for inducing the therapeutic anti- Aβ antibodies without inducing strong Th1 responses. The new vaccine candidates contain “molecular adjuvants” that can amplify the humoral (antibody) response while attenuating the potentially dangerous cell-mediated immune response against Aβ. These include both peptide based and DNA based immunization strategies that include components of the complement system, innate immune system and cytokines in order to enhance antigen presentation and to polarize the immune response toward a Th2 phenotype. New pre-clinical trials in APP/Tg mice are critical for gaining a better understanding of the potential benefits, as well as, the risks associated with Aβ-immunotherapy as a strategy for treating AD patients.
Students must be interested in laboratory research and motivated to complete an independent project. Research involves animal handling, behavioral testing, blood and solid tissue collection, fixation and staining of brain tissue and characterization of neuropathology. Students will also participate in vaccinating mice, analyzing serum by ELISA for antibodies against the beta-amyloid peptide. Interested students should submit an updated resume along with a list of completed chemistry, biology and neuroscience courses. Project assignments will be determined by the commitment and enthusiasm of the student for lab work. Interested students are encouraged to visit the lab prior to requesting a position. Minimum requirements: 1) GPA of at least 3.0 2) Minimum commitment 1 year (4 quarters), 2 years preferred. 3) Must commit to work 8-12 hours per week, attend weekly lab meetings and present their research results during lab meetings. In addition, previous lab experience and competence with Photoshop, PowerPoint, Excel and statistical analysis are desirable. The quality of laboratory work, lab meeting presentations and a quarterly paper on research results will figure eqully in the grade evaluation for the 199 students.
Elysia Davis, Ph.D.Psychiatry & Human Behavior and Pediatrics
RESEARCH DESCRIPTION: We are evaluating the consequences of prenatal exposure to stress and stress hormones for fetal, infant and child development in several NIH funded research protocols. Students working on this project will have the opportunity assist with data collection for several fun and interesting studies of child development. Prior experience working with children is preferred, but not required. We are looking for highly motivated students with an interest in medicine, psychology, biology, or public health, who are willing to work 10-15 hours/week for a minimum of 1 year. Responsibilities will include: administering assessments of child temperament, conducting standardized interviews, evaluating biological and psychosocial indicators of maternal and child stress, and database management. Training will be provided. Students should have access to transportation to and from the Medical Center.
REQUIREMENTS TO PARTICIPATE: Transportation to and from the research site, minimum one year commitment, reliable, an interest in working with young children.
COURSE COMPLETION: N/A
GRADE POINT AVERAGE: N/A
TIME COMMITMENT PER WEEK: 10-15 hours per week
NUMBER OF UNITS REQUIRED: at least 3 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: hours completed and quality of work completed. OFFICE LOCATION: 333 City Blvd. West, Orange suite 1200
Hamid Djalilian, Otolaryngology-Head and Neck Surgery
RESEARCH DESCRIPTION:
The purpose of this project is to use a novel method to stimulate the facial, hearing and balance nerves using a penetrating electrode. Restoring hearing, balance, or facial function will be attempted by stimulating the respective nerves in an animal model.
REQUIREMENTS TO PARTICIPATE:
Life sciences or engineering major or concentration
GRADE POINT AVERAGE: 3 or higher
TIME COMMITMENT PER WEEK: 8 hrs
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Completion of assigned tasks, feedback on experiments, reading. OFFICE LOCATION: UCI Medical Center Bldg 56, Suite 500
We study a mouse model of inflammatory bowel disease. The mouse is knocked out of the pertussis-toxin sensitive G-protein alpha subunits, Gia2. These animals spontaneously develop inflammation in the colon after 6 weeks of age which mimics Crohn’s disease; these mice subsequently develop colon cancer. Our work has identified a number of signaling abnormalities whose potential contribution to the development of disease is being studied. We are interested in how the lack of Gia2 affects the development of the immune system, the intestinal epithelium, and how different cell types (epithelial, stromal, and lymphoid) that lack Gia2 interact in regulating mucosal immune responses and the development of cancer in these mice. The work involves mouse husbandry, animal and tissue culture experiments, nucleic acid protein work, and an array of analyte determinations. Completed standard prerequisites for Bio 199, at least a sophomore or greater. Prefer students with expressed interest in aspirations for advanced degrees in biological sciences. GPA 3.3 or greater. Prefer at least a 2 quarter commitment. 8-10 hours/wk, 4-5 hour blocks preferred.
Gregory, R.D. Evans, M.D., F.A.C.S.Aesthetic and Plastic Surgery Institute
RESEARCH DESCRIPTION:
The focus of our lab is tissue engineering. Herein, we work in several different fields: 1. Genetically transfected human embryonic kidney stem cells to produce NGF secretion and to improve peripheral nerve regeneration; 2. Improvement of peripheral nerve regeneration by human adipose derived stem cells differentiated into neural lineage cells; 3. Direct differentiation of human adipose derived stem cells into beta-islet cells; 4. Microcirculation in free flaps, impact of tissue harvest and ischemia reperfusion injury; 5. Improvement of wound healing by enhanced cryoprecipitate; 6. Early detection of vascular thrombosis and disturbances by modulated imaging. Students who are interested will learn about basic techniques in cell cultures, molecular techniques as RT-PCR, ELISA, immunohistology, immunocytology, Western Blot, histomorphometry, and will be involved in animal models including functional evaluations of nerve injuries, etc..
REQUIREMENTS TO PARTICIPATE:
3 quarter minimum commitment, willing to work with rats, some knowledge in medical statistics, experiences in molecular techniques preferred.
COURSE COMPLETION:
Standard prerequisites for Bio 199
GRADE POINT AVERAGE:
3.5 or greater
TIME COMMITMENT PER WEEK:
At least 10 hours per week, Blocks of 2-3 hours minimum
NUMBER OF UNITS REQUIRED:
3 minimum
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Attendance, quality of work, dedication, empathy. OFFICE LOCATION: 200 S. Manchester Ave., Suite 650, Orange, CA 92868
RESEARCH DESCRIPTION: You will have an exciting opportunity to learn about the importance of early social development among newborns, infants, and toddlers. You will also become well-versed in the utility of cutting-edge eye-tracking methodology and dynamic behavior coding of dyadic interactions. Additionally, you will be familiarized with standardized psychosocial assessments, which are commonly used for the identification and diagnosis of special needs in young children. Moreover, you will be able to observe clinical visits with a developmental pediatrician, pediatric neurologist, nurse practitioner, and pediatric speech-language pathologist.
REQUIREMENTS TO PARTICIPATE: (1) Interest in child development (e.g., early social-interactive behaviors), pediatrics, neurology, or ophthalmology; (2) Willingness to learn the use of advanced methodology in early psychosocial development (e.g., behavior coding software, eye-tracking); (3) Must be responsible and willing to work collaboratively in an interdisciplinary, professional setting; (4) Must demonstrate the ability to work independently; (5) Must be willing and able to commit to a minimum of 12 months (i.e., freshmen, sophomores, juniors, and seniors are encouraged to apply).
COURSE COMPLETION: N/A
GRADE POINT AVERAGE: Minimum 3.0 GPA
TIME COMMITMENT PER WEEK: Minimum 8 hours per week on either Tuesday and/or Wednesday during Spring 2009 (scheduling of subsequent sessions to be determined)
NUMBER OF UNITS REQUIRED: Minimum 2 units per quarter (4 hours/unit)
OTHER: Must have reliable transportation to For OC Kids in Orange, CA
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: 1) Participation in research activities (e.g., behavior coding) (45%); (2) Completion of individual term paper and/or Excellence in Research (45%); (3) Attendance and Professionalism (10%). OFFICE LOCATION: For OC Kids Neurodevelopmental Center, 1915 W. Orangewood Avenue, Suite 210, Orange, CA 92868
TELEPHONE: Please correspond by emailing phanjt@uci.edu
Contact:
Jennifer Phan, Project Coordinator: phanjt@uci.edu, (000) 000-0000
Mark Fisher, M.D.Neurology and Anatomy & Neurobiology
RESEARCH DESCRIPTION:
Laboratory of Stroke and Vascular Neurobiology: The laboratory focuses on fundamental mechanisms of stroke, the third leading cause of death in North America. Stroke is usually caused by blood clots blocking arteries of the brain. The laboratory investigates how blood clot formation is regulated by the brain, a process referred to as “brain specific hemostasis regulation”. We use cell culture models of the brain microvasculature (“blood-brain barrier”) and animal models of experimental stroke.
REQUIREMENTS TO PARTICIPATE: At least two quarter commitment
COURSE COMPLETION: Basic understanding of biological sciences
GRADE POINT AVERAGE: 3.0 or higher
TIME COMMITMENT PER WEEK: 8 hours minimum
NUMBER OF UNITS REQUIRED: 1-4 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Oral and written presentations. OFFICE LOCATION: Shanbrom Hall, Room 121, UC Irvine Medical Center
Lisa A. Flanagan, Pathology and Laboratory Medicine
RESEARCH DESCRIPTION:
Neural stem cells hold great promise as potential therapuetic agents for a variety of central nervous system diseases and injuries. Our lab is interested in intrinsic and extrinsic regulators of cortical neural stem cell proliferation, neurogenesis, and neurite outgrowth/regeneration. Projects will utilize cell culture, immunocytochemistry, microscopy, collaborations with colleagues in biomedical engineering, and data analysis. Resumes with a completed course list can be sent by email (lflanaga@uci.edu).
REQUIREMENTS TO PARTICIPATE: interest in cell biology and neuroscience, highly motivated, good organizational skills
COURSE COMPLETION: Bio 194S, preferred completion of laboratory coursework
GRADE POINT AVERAGE: 3.0 minimum
TIME COMMITMENT PER WEEK: 10+ hours/week, 1 year comittment
NUMBER OF UNITS REQUIRED: 3 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: completion of assigned tasks, understanding of biology, communication skills. OFFICE LOCATION: D433 Medical Sciences D
Cerebral cortex development and cortical neural stem cell biology
Projects with Dr. Flanagan will investigate intrinsic and extrinsic regulators of cortical neural stem cell proliferation, neurogenesis, and neurite outgrowth/regeneration. Projects will utilize cell culture, immunocytochemistry, microscopy, and data analysis. Requirements: Appropriate background in biology with interest in cell biology and neuroscience, good organizational skills, 10+ hours/week, 1+ year commitment, preferred previous lab coursework and GPA 3.0 or higher. Resumes with a completed course list can be sent by email
Topic: emotion and mental illness. Study of people's reactions to emotional material through subjective ratings, psychophysiological responses and brain imaging. Experience with these patient groups: depression, schizophrenia, Alzheimer's, etc. Some work done at the UCI Brain scanning. Prefers commitment of two years and students interested in neuroscience, mental illness, and brain imaging.
Requirements: GPA 3.5 or better; must be willing to commit at least 15 hours/week and a minimum of 1 year.
Description: The laboratory focuses on immune responses to viruses, particularly HIV. We study how antibodies interact with receptors on natural killer cells and macrophages and thereby inhibit viral replication. Students perform a number of techniques including PCR, cell-surface staining, cell culture, and ELISA. Students do not deal directly with HIV. It is hoped that student's can develop their own projects.
Requirements: 3.5 overall GPA, sophomore standing, Friday availability, and two year commitment. Four hours/unit/wk. Minimum of 3 units. Ability to work independently as well as in a group setting. Description: Emergency Ultrasound involves the use of portable ultrasound machines at the bedside of patients in the emergency department. The new field requires clinical research that supports its implementation by proving its safety and efficacy in a wide variety of applications. Currently we have protocols involving pediatric trauma, ocular ultrasound to asses for intracranial pressure, biliary ultrasound, and first trimester complications of pregnancy. 199 students are expected to attend the weekly research meetings held on Friday afternoons in Dr. Fox's office. Also, accuracy of data entry/analysis with an emphasis on clinical application will be tested. Opportunities for publication are available.
RESEARCH DESCRIPTION: The focus of our lab is cortical plasticity, which is the ability of the cerebral cortex to continuously modify itself, and therefore is fundamental for understanding higher brain functions including learning and memory. We use functional imaging, electrophysiological recordings, anatomical and behavioral techniques to study plasticity and its underlying mechanisms. Our research interests include studying naïve rats as well as rats whose life experience has changed in some way, such as in response to living in a more ‘naturalistic habitat’ rather than a laboratory cage or in response to injury such as a stroke. See our website for more details on our lab’s research. Project assignments will be determined based on the student’s commitment and enthusiasm and include data analysis, anatomy, assisting with imaging experiments, and/or establishment/maintenance of the naturalistic habitats.
REQUIREMENTS TO PARTICIPATE: Minimum 1 year commitment; minimum 2nd year student; willingness to work with rats/mice.
GRADE POINT AVERAGE: minimum 3.3 GPA
TIME COMMITMENT PER WEEK: minimum 6 hours/week
NUMBER OF UNITS REQUIRED: minimum 2 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Attendance; productivity; quality of work; enthusiasm. OFFICE LOCATION: 2219 McGaugh Hall
Description: Students will carry out in vitro cell line work to determine the effects of anticancer agents on tumor cell and tumor vascular cell growth. Antiangiogenesis models will be emphasized. Cancer pharmacology will be reviewed. For some studies, biomarker correlates with drug response will be determined using immunohistochemistry, image analysis, flow cytometry, gene expression arrays and proteomics techniques. Students will be expected to define a hypothesis driven project, present a15 minute lecture summarizing their objectives, prepare a mid term oral and written report, and present a final oral and written report. When data are of adequate quality, the student will be encouraged to submit a manuscript for publication and to present their work at the UCI science fair. Prerequisites: Introductory Biology and Chemistry; Bio 194S; Cum GPA>3.0. Organic Chemistry and Biochemistry preferred.
Pietro Galassetti, M.D., Ph.D.Pediatrics, General Clinical Research Center
RESEARCH DESCRIPTION: Our lab currently has 4 human research protocols funded by the NIH and/or JDRF. 1) We are researching the adaptive responses to exercise in Type 1 Diabetic children/adolescents with hypoglycemia and/or hyperglycemia. Cytokines such as (IL-4, IL-6, IL-1α, IL-1β, TNF-α, ), growth factors (IGF-I, IGF BP-1), and neuroendocrine components (Glucagon, Cortisol, GH, Epi, Nor-Epi) are measured via ELISA and LUMINEX assay techniques. 2) The second protocol is aimed at defining the inflammatory and oxidative stress responses during exercise in children with obesity/metabolic syndrome, and in healthy controls, in fasting conditions and after ingestion of a high-fat meal. Data will be analyzed attempting to ascertain the effects of maturational status, gender, and physical fitness. In addition to the assays listed above, a broader panel of oxidative markers will be used, including measurement of leukocyte gene expression. Assessment of total and regional body composition by DEXA, and assessment of the cardio-respiratory responses to exercise will also be performed. 3) The third protocol is a joint effort with the Blake/Rowland lab in the Department of Chemistry to define and identify correlations between exhaled breath gas profiles, plasma glucose, insulin, FFAs, ketones and oxidative stress markers in healthy and Type 1 Diabetic adults, in an effort that may lead to the creation of new techniques allowing the accurate, non-invasive measurements of these variables. In addition to the assays listed above, a sophisticated gas chromatography/mass spectrometry analysis of expired gases is performed. 4) The fourth protocol researches the effects of exercise on the immune system’s responses in adult males. Strenuous exercise is used to stimulate cellular and humoral changes through recruitment and activation of blood leukocyte subpopulations. For key immuno-modulatory mediators, in addition to systemic circulating concentrations, also levels of gene expression and intracellular concentrations in leukocyte subpopulations will be studied.
REQUIREMENTS TO PARTICIPATE: Students interested in clinical research. Enthusiastic, responsible individual who demonstrates a willingness to learn. Requires working an occasional weekend day (approximately 1-2 days per month). Good organizational and time management skills are crucial. Students should have access to transportation to and from the Medical Center. A commitment of at least two consecutive quarters is necessary.
COURSE COMPLETION: Completion of standard prerequisites for 199.
GRADE POINT AVERAGE: Minimum 2.5 GPA
TIME COMMITMENT PER WEEK: 8-12 hours/week
NUMBER OF UNITS REQUIRED: 2-3
OTHER: Students will have the opportunity to learn about working with human subjects in clinical research environment. Review of ongoing research projects (grants) and UCI regulatory documents (IRB, GCRC). Under supervision, the student will assist with (including but not limited to) plasma analysis on Beckman Glucose Analyzer, recording and entering of data in existing databases relating to research studies, and review of pertinent literature. Structure and organize data into presentation formats for national and local meetings, journal club meetings and scientific manuscript submissions. Will meet weekly with PI, staff, and participate in department and laboratory meetings. Training will be provided.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance, quality of work and performance of assigned tasks, participation in laboratory meetings, and acquired knowledge of research field. OFFICE LOCATION: 1111 Hewitt Hall. If interested, please contact Rebecca Flores (rlflores@uci.edu, 949-824-4433) and cc Dr. Galassetti (pgalasse@uci.edu) on your correspondence."
RESEARCH DESCRIPTION: Our research utilizes novel systems-level approaches to identify and characterize the genes that govern melanin pigmentation and the genes that control melanoma chemoresistance. The ultimate goal of these studies is to identify novel agents to treat melanoma and disorders of pigmentation.
REQUIREMENTS TO PARTICIPATE: minimum sophomore standing
COURSE COMPLETION: must have completed 100L, 114L, or 116L
GRADE POINT AVERAGE: 3.5
TIME COMMITMENT PER WEEK: 16- 20 hours
NUMBER OF UNITS REQUIRED: 3-4 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Evaluation will be based on ability to utilize the scientific method to design and critically evaluate the results of experiments performed in the laboratory.OFFICE LOCATION: 310A Med Surge II
Role of signaling pathways in the induction of scar-free wound healing and limb regeneration. Successful completion of first year courses – strong interest in regeneration. GPA 3.0. Minimum of 10 hours/wk. Typically 4 units.
*Note: Enroll in course code under Dr. Susan Bryant.
Jean Gehricke, Ph.D.Psychiatry and Human Behavior, Child Development Center
TITLE: Research on smoking and ADHD.
RESEARCH DESCRIPTION: Dr. Gehricke’s research focuses on why individuals with ADHD have higher smoking rates and lower cessation rates compared to the general population. Learn about the effects of nicotine and cigarette smoke on human behavior and brain circuitry as well as the genetic and environmental risk factors that lead to smoking initiation and nicotine addiction. Students will be trained in implementing study protocols, data collection techniques, data entry, and analysis. Good opportunity for students interested in careers in medicine, clinical psychology, and drug abuse treatment and prevention.
REQUIREMENTS TO PARTICIPATE: Minimum commitment of one year.
TIME COMMITMENT PER WEEK: 8-10 hours/ week.
NUMBER OF UNITS REQUIRED: 3-4.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Reliability and consistency in attendance. Completion of assignments and required hours. Discussions of research papers. Brief paper in one area of drug abuse research. OFFICE LOCATION: 19722 MacArthur Blvd., Irvine.
Paul Gershon, Ph.D.Molecular Biology & Biochemistry
I am active in four areas: (1) We operate a protein (roteomics? mass spectrometry facility containing two powerful, state-of-the-art tandem mass spectrometers along with nanoflow/nanocapillary two-dimensional peptide fractionation HPLC with robotics. Last year, several 199s deftly learned to operate these instruments and used them to, among other things, identify all the proteins in some of the world largest viruses. We will now be identifying and quantitating the proteins characteristic of diseased/tumorigenic cells and developmental abnormalities. (2) My lab is currently funded to investigate the structure and molecular dynamics of the enzyme poly(A) polymerase (PAP), using the vaccinia virus enzyme as a model. The vaccinia PAP was the first PAP for which a gene was identified (by the P.I.), and we recently learned its three-dimensional structure. Vaccinia PAP is the only known polymerase that can translocate independently on single-stranded nucleic acid. How does it do this on a non-rigid polymer? Come to my lab and solve the mystery. (3) A collaboration between my lab and that of Dr. Alex McPherson (MB&B) has led to the clearest images obtained thus far of vaccinia virus particles and subviral assemblies, providing a unique opportunity to identify viral proteins and functions present in various subviral assemblies. This dovetails well with protein mass spectrometry (above). (4) I am also funded to investigate the catalytic mechanism of RNA O-methylation. Vaccinia virus protein VP39 provided the first three-dimensional structure for any poxvirus protein and any RNA methyltransferase. The structure was then solved again, with bound RNA substrate and cofactor. Using various chemical, biochemical and NMR techniques, we are elucidating aspects of its catalytic mechanism from hypotheses arising from the three-dimensional structure. Overall, prior undergraduate interns in my lab have been included as authors on manuscripts submitted for publication, and some current ones will be.
Students will work on research studies involving the role of biological and psychological processes in pregnancy and on prenatal factors influencing later psychiatric health. The research protocols are varied and students will participate in EEG, archival chart review and data analysis. Students who are willing to make a three quarter commitment are preferred. Training will be provided.
RESEARCH DESCRIPTION:
Our research focuses on the role of Toll Like receptors (TLRs) in Breast cancer cell survival. These receptors are pattern recognition receptors. Pattern recognition receptors are expressed on a wide variety of cells and they promote phagocytosis. TLRs signal via conserved pathways to induce [1] secretion of a number of proinflammatory cytokines, [2] apoptosis, and [3] potentiate apoptosis induced by anti-cancer agents. The expression of TLRs in breast cancer cells is suggested by the capacity of breast cancer cells to phagocytose yeast (which is recognized by TLR 2 and dectin, a PRR that detects beta glucan of yeast cell wall) and induction of apoptosis in breast cancer cells by yeast cell wall components. Students will gain experience in a range of research techniques including cancer cell biology , molecular biology and Biochemistry
REQUIREMENTS TO PARTICIPATE: Students beginning in summer and continuing through the year will be given preference
COURSE COMPLETION: Basic course work in Biology and Biochemistry
GRADE POINT AVERAGE:3.0 and above
TIME COMMITMENT PER WEEK: 16-24 hours /week
NUMBER OF UNITS REQUIRED: 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
The quality of laboratory work and presentation of their research findings, both written and oral, in the lab meetings will figure eqully in the grade evaluation. OFFICE LOCATION: Room C348, MedSCi Bld 1
My research is based on structure-function determination of Mycobacterium tuberculosis proteins in the hope of finding novel anti-TB drug targets. The techniques that we utilize include X-ray crystallography, biochemical characterization and mass spectrometry. My main focus is on a mycobacterial iron up-take system that scavenges iron from humans in the form of heme, and then uptakes this heme to be broken down for iron usage either within the bacterial membrane or cytosol The student research projects may include cloning, protein expression and purification and crystallization trials initially. There will also be some protein-protein interaction experiments to carry out. Completion of Bio 194S (biosafety and ethics) is required and a basic course in biochemistry/chemistry would be preferable. Must be highly motivated and be prepared to work12 hours/wk. 3 units. GPA 3.2 or better.
Two positions available. One would work on molecular biology technology involved in dissecting some novel gene from two infants with unique congenital immunodeficiency and other for molecular mechanisms of cell death. Should have minimum GPA of 3.8 who are majoring in Biology.
Project involves a study of the global pattern of species diversity for birds. Students must be willing to work at least 6 hours per week (3 hours per unit). Students who work on project a second year are expected to conduct a small independent study related to the major project.
RESEARCH DESCRIPTION:
Orthopaedic Oncology Lab focusing in molecular biology and genetics of bone and soft tissue tumors. Techniques include DNA and protein gel electrophoresis, tissue culture, gene transfection, microscopy, biological assays, immunohistochemistry.
REQUIREMENTS TO PARTICIPATE: complete Clinical package to work at UCIMC laboratory.
COURSE COMPLETION: Bio. 194 Safety & Ethics
GRADE POINT AVERAGE: 3.3 or above
TIME COMMITMENT PER WEEK: 3-4 hours per week for each credit
NUMBER OF UNITS REQUIRED: 1-5 credits
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: completion of research proposal, attendance, presentation at lab meeting, attending monthly lab meetings, reading journal articles. OFFICE LOCATION: UCIMC, Pavillion 3, second floor.
RESEARCH DESCRIPTION: Students will be involved in research investigating the role of Wnt signaling pathway in human cancer. A primary area of focus is colon carcinogenesis. Studies investigating the activity of a natural compound, resveratrol, on the Wnt pathway are also ongoing in the laboratory. Students will work under the direction of Dr. Holcombe and in collaboration with several post-doctoral fellows and research associates in the laboratory. Molecular and cell biology techniques will be utilized in this research.
REQUIREMENTS TO PARTICIPATE: Ability to travel to the Cancer Center in Orange where the laboratory is located.
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 10-12 hours
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Supervision of progress on assigned projects, written reports, presentations to laboratory group and abstract preparation. OFFICE LOCATION: UCIMC, Building 56, Rm 247
RESEARCH DESCRIPTION: Circadian Rhythms
REQUIREMENTS TO PARTICIPATE: Student interview
COURSE COMPLETION: BIO 194
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 10 hour minimum
NUMBER OF UNITS REQUIRED: 3 - 4 (3 minimum)
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Student presentation and Lab meeting attendance. OFFICE LOCATION: D340 Medical Science I, Irvine, CA 92697-4560
RESEARCH DESCRIPTION: We study how protein complexes regulate ion channels. Students will have opportunity to participate in experiments covering various aspects of pharmacological research: molecular (cloning) and cellular (cell culture) biology (western blot) and electrophysiology (if desire).
REQUIREMENTS TO PARTICIPATE: students interested in pharmacological/pharmaceutical research; Preference to those with experience in biological and/or chemical techniques.
COURSE COMPLETION: Course requirements vary by project.
GRADE POINT AVERAGE: 3.3
TIME COMMITMENT PER WEEK: > 8 hrs/week
NUMBER OF UNITS REQUIRED: >2
OTHER: 360 C Med Surge II
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: participation, willingness to learn, responsibility. LOCATION OF RESEARCH: 309 Med Surge II
Our research focuses on developing and employing mass spectrometry-based proteomic approaches to answer a series of biological questions related to cancer diagnostics and treatment. Current projects include characterization of proteasome complex composition, their post-translational modifications and mapping protein interacting network in normal and cancer cells; investigation of protein ubiquitination and ubiquitin-like protein modifications to understand their functions in turmorigenesis. Requirements: completion of organic chemistry, cell biology, biochemistry. GPA 3.0 or higher, one year commitment.
David Imagawa, M.D., Ph.D., FACSHepatobiliary and Pancreas Surgery
RESEARCH DESCRIPTION:
The Division of Hepatobiliary and Pancreas Surgery involves examination of various conditions and treatments affecting diseases of the liver, pancreas, and biliary tree. Students will be expected to attend weekly clinics on Monday mornings and Thursday afternoons, as well as work independently in the office. Student tasks will include maintaining the research database, statistical analysis, and other research duties as necessary. Ability to learn new applications. Experience with Excel, Access, SPSS, Adobe Premiere, Adobe Photoshop, Adobe Illustrator preferred. Must be willing to travel to medical center.
Student will be supervised by the PI and the surgical research resident.
REQUIREMENTS TO PARTICIPATE: 3.0 overall GPA and sophomore standing
COURSE COMPLETION: Bio 194S. Statistics and physiology recommended but not required
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: Approximately 12 hours/week
NUMBER OF UNITS REQUIRED: 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Quality of work, fulfillment of time commitment OFFICE LOCATION: 101 The City Drive South Bldg 56 Room 202 Orange, CA 92868
The use of animal models for screening and evaluating anti-aging pharmaceutical and botanical compounds is a promising approach for drug discovery. While testing anti-aging compounds using the premier animal genetic systems, Drosophila melanogaster and Caenorhabditis elegans, has already started, the fundamental biological issues involved in such screening have not been systematically formulated. As a result, after selecting potential anti-aging compounds to be tested, we need appropriate methodologies to study the pharmacology of aging in model species. Once these methodologies or rules of investigation are developed, we may consider extrapolating these experimental findings with such systems to the treatment of human aging. However, there are a number of potential artifacts, confounds, and errors that can arise in such research programs. In order to minimize these problems, I developed the following assays as the rules of investigation in anti-aging pharmacology and am in the process of applying them to a number of potential anti-aging compounds. These are the rules of investigation that I propose: Since human adulthood is almost exclusively a period of aging, data that conflate aging and late life should not be extrapolated to human aging. 2) The response to candidate medications should show a normal drug-dose response pattern, although not necessarily a linear response. 3) Medicated animal models should not be hypometabolic. 4) Medicated animal models should not show pronounced reductions in fertility. 5) Medicated animal models should not exhibit general nervous system depression. 6) The effect of the medication should not be highly sensitive to the culture environment. 7) The effect of the medication should not be highly dependent on the genetic ancestry of the stock employed, leaving aside inbreeding, which should be avoided because humans are not generally inbred. While these rules do not guarantee successful extrapolation of successful drug results from the animal model to humans in a clinical setting, the failure to adhere to these rules should raise doubts about such extrapolation. In my research, I attempt to address this task. Interest in a career in biomedical research is required. All students start as Bio. 198, group research students. Progress to Bio. 199 status depends on the student's ability to work independently. NO student begins in the Bio. 199 course. 3-4 units required involving 10-12 hours/week. A 2-year commitment and a desire to apply for a UROP grant, SURP fellowship, and Excellence in Research is recommended. Must be highly motivated to complete an independent project while working closely with the research staff and PI. Biology and Chemistry Majors preferred with a minimum 3.3 GPA.
RESEARCH DESCRIPTION:
Examination of, and maintenance of, melanoma center patient data base; outcome of research: accuracy, failure, recurrence, survival and complications in sentinel lymph
node mapping.
REQUIREMENTS TO PARTICIPATE: only BID 194
COURSE COMPLETION: per semester / 3-5 page paper
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 3–4 hours/week
NUMBER OF UNITS REQUIRED: 3–4
OTHER: N/A
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Accuracy of data entry / chart review success in focus on a specific clinical outcome. Example: # of patients with lymphocele or lymphodema; or, false negative lymph
node basin rate / recurrence in a node basin originally staged clear. OFFICE LOCATION: UCIMC Bldg 56
Sunny Jiang, Ecology and Revolutionary Biology, Civil and Environmental Engineering
Research in Dr. Jiang's lab focuses on the following three areas: 1) aquatic/marine microbial ecology, 2) water quality microbiology, 3) epidemiology of exposure to recreational waters.
Her group uses molecular method to uncover the genomes and interaction of organisms in the environment. Over the past decade, her team has developed rapid and sensitive methods for detection and quantification of human virus contamination in aquatic samples. Their results have suggested that current coastal water standards do not adequately reflect the viral quality of recreational waters. Working together with environmental engineers, coastal physical oceanographers and remote sensing scientists, they have recently discovered that surf zone water quality is impacted by multiple factors including global climate changes, land use patterns and human activities. Working directly with the City of Newport Beach and Regional Water Quality Control Board, they have investigated the relationship between fecal indicator bacteria and human viruses in Newport Bay. The results showed that the occurrence of these two types of organisms is uncoupled largely due to the differences in the source and survival of each type. Another area of her research addresses human health risk from exposure to coastal waters. Her research group is currently developing web-based survey tools to understand the relationship between human health risk and coastal water quality. Sample projects in her lab include investigation the causes and decline of harmful algal blooms (red tides), detection of pathogens in water, epidemiological surveillance of recreational illnesses.
REQUIREMENTS: Students should be prepared to develop independent projects and spend at least 10 hours per week on research. A multi-quarter commitment is strongly preferred, and students will be expected to participate in lab activities and meetings. MEANS OF EVALUATION: grades will be based on time committed to the project and the quality of research.
RESEARCH DESCRIPTION: Medical imaging methods provide a unique tool for the quantitative evaluation of the healing process, even when brought about by non-conventional means. Our ongoing research is investigating subtle energy medicine using contemporary medical imaging and laboratory methods. Here “subtle energy” refers to a set of modalities, such as acupuncture and Pranic Healing, in which the mechanisms of action are not well understood. Students would have the opportunity to participate in various aspects of this ongoing work depending on their background and interest. Activities range from library/internet searches through the statistical analysis and evaluation of large data sets.
REQUIREMENTS TO PARTICIPATE: Depends on specific project undertaken. Several projects require a strong background in mathematics and physics.
COURSE COMPLETION: Depends on specific project undertaken.
GRADE POINT AVERAGE: 3.5 although exceptions may be made.
TIME COMMITMENT PER WEEK: 9 to 12 hours per week.
NUMBER OF UNITS REQUIRED: 3 to 4 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: (1) active participation in regular meetings and (2) on-time completion of assigned tasks (which may involve a written report).
RESEARCH DESCRIPTION: Students will have the opportunity to perform clinical or translational investigations with a focus on pancreatic cancer. My newly-established laboratory focuses on the evaluation and study of novel treatment options for patients with this disease. I work with both in vitro and in vivo mouse models. Students with an interest in such benchside scientific activity will be responsible for working on a current project helping to design a new project from the ground up. Students will clinical interest may choose instead to focus on database mining to answer a clinical question relating to pancreatic cancer. Attendance at clinic on Friday afternoons is open to those with an interest, but is not obligatory. Presentation and publication of clinical or translational findings is expected.
REQUIREMENTS TO PARTICIPATE: Bio 194S
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 4 hours/unit
NUMBER OF UNITS REQUIRED: 3-4
OTHER: Contact Matthew Katz or Brian Nicholson, 714-456-8899 or email katzmh@uci.edu
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of work, fulfillment of time commitment. OFFICE LOCATION: 333 City Boulevard West, Suite 1205
Bio199 students within the Keirstead lab take part in a variety of different tasks ranging from assisting in surgical procedures such as spinal cord contusion injuries, and intra spinal cellular transplantations to tissue processing, immunohistochemistry and data analysis. Students will often find themselves conducting behavioral tests, summarizing the data into Excel spreadsheets, performing routine post-op animal care and monitoring. These tasks may include administering saline and antibiotic injections intraperitoneally or subcutaneously, daily injections with an immunosuppressant drug, and the quantitative analysis of animal kinematics by analyzing videotaped images. Research will also include tissue dissection, cryostat sectioning, and numerous molecular biology techniques that may include DNA concentration, PCR, electrophoresis, enzyme digestion along with the preparation of cell culture media and gels. Students must be highly motivated, will be expected to dedicate an average of 12-15 hours per week, and must be willing to work on weekends on occasion. Experience in the following areas would be appreciated but not necessary: cryostat cutting and immunological staining, previous animal handling experience or willingness to work with live animals, and previous academic course work in the neurobiology/biology field.
Optimizing current treatments and developing alternative therapies for port wine stain birthmarks (PWS). The current treatment of choice for PWS is the pulsed dye laser (PDL). However, multiple treatments are required and less than 15% of lesions are cleared completely. We are developing ways to improve current treatments including non-invasive imaging of blood vessels and the use of anti-angiogenic factors. We are also exploring novel treatment options including photodynamic therapy. Preliminary evaluations are done on a rodent dorsal skinfold model and then promising approaches are further evaluated in clinical trials.
COURSE COMPLETION: Preferably beginning engineering and/or biology courses.
GRADE POINT AVERAGE: No requirement
TIME COMMITMENT PER WEEK: 10-20 hours/week
NUMBER OF UNITS REQUIRED: No requirement
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Performance during the project – specifically involvement in data collection and manuscript preparation.
RESEARCH DESCRIPTION:
Dr. Virginia Kimonis is the new chief of the Division of Genetics and Metabolism in the Department of Pediatrics at UCI. While involved in many aspects of clinical genetics and clinical research (multicenter clinical NIH studies involving natural history and genotype- phenotype studies in Prader-Willi and Craniosynostosis syndromes), she has an international reputation for her clinical and laboratory research work in inherited muscle diseases that occur in combination with diseases of bone and/or dementia (also known as IBMPFD) Her group localized the gene for the disorder to chromosome 9, identified the causal gene as VCP (CDC48 or p97) and are now identifying the key pathways and functions that are disrupted by the mutations they have found in the affected families. They are also developing a knock-in animal model of the disorder and are working on developing better clinical evaluations, diagnostic testing and treatments for families with IBMPFD, but also for those with other sporadic and hereditary diseases that share components of IBMPFD. OFFICE LOCATION: Hewitt Hall, Irvine and Tower Bldg, Orange
Contact:
Virginia Kimonis: vkimonis@uci.edu, (714) 456-5791 or (949) 824-0571
John Krolewski, MD- PhDPathology
Our lab is investigating mechanisms of cytokine signaling in both normal and cancerous cells. The experimental systems include both cells in culture and rodent models. In one set of experiments we are investigating an unusual signaling mechanism involving proteolytic cleavage (proteolysis) within the plasma membrane. Another project involves the determining how the natural product Madindoline acts to block IL6 signaling. IL6 is a growth factor for some tumors, including prostate cancers. Finally, in a third project, we are investigating the mechanism of androgen withdrawal induced cell death in prostate epithelial cells. The same mechanisms may be involved in the death of prostate cancer cells. Students will be assigned individual projects and will work under the mentorship of Dr. Krolewski and/or other personnel in the lab. A minimum of a 15 hr/wk commitment is required, for at least 6 quarters. Sophomores interested in making a long term commitment are preferred.
Young Jik Kwon, Ph.D.Pharm Sci; Chem Engnring & Materials Sci
RESEARCH DESCRIPTION:
Timely, complete, and convenient treatment of many diseases is not yet within reach, although many potent therapeutics have been identified and developed. Successful delivery of therapeutics depends on finding paths toward ideal forms of treatment. In BioTherapeutics Engineering Laboratory (BioTEL) in departments of Pharmaceutical Sciences, and Chemical Engineering and Materials Science tackles this problem by identifying the limitations of current therapeutics and the reasons for those limitations. Target therapeutics are then selected and conjugated in the form of micro- and nanoparticles, using organic chemistry and carrier fabrication tools. BioTEL is currently working to develop drug delivery systems that selectively localize therapeutics in the body, down to the level of cellular compartments, and offer controlled release of therapeutics so that drugs can be delivered only at the desired time. BioTEL employs techniques to immobilize therapeutics, including cancer chemotherapy agents, imaging probes, therapeutic genes and proteins, and cells. Once carriers are localized in a target site through passive and active targeting mechanisms, therapeutics can be triggered to be active by pathological signals. Synthesis of novel carrier materials, innovative fabrication of carriers, elucidation of extracellular and intracellular behaviors of drug/carriers, and development of new drug release mechanisms are the key scientific emphases in BioTEL. Opportunities for undergraduate researchers including nanocarrier synthesis and characterization as well as in vitro and in vivo tests, depending on needs in the lab and qualification of participants.
REQUIREMENTS TO PARTICIPATE: Basic molecular biology and organic chemistry
COURSE COMPLETION: Sophomore and juniors are ideal but seniors with previous experience will also be considered.
GRADE POINT AVERAGE: 3.0 or higher
TIME COMMITMENT PER WEEK: Minimum 10 hrs spread through weekdays and weekends
NUMBER OF UNITS REQUIRED: 3 or more
OTHER: Students with intention of a long-term participation will be given high priority.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Written reports, literature review, presentation in the group meeting, evaluations from graduate co-workers. OFFICE LOCATION: Engineering Tower 944F
RESEARCH DESCRIPTION: Two research opportunities: 1) Participate in clinical outcomes studies at the For OC Kids Neurodevelopmental Center in Orange. Studies involve children under the age of six with Autism, ADHD, and other neurodevelopmental disorders. We are looking for enthusiastic students with an interest in pediatrics who are willing to commit to 9-12 months of training for approximately 8-12 hours per week. Training components include: a) Introduction to neurodevelopmental disabilities and evidence-based treatment through seminars, readings, and clinical observation, b) Training in clinical outcomes evaluation methods, c) Participation in outcomes evaluation (data collection, entry, analysis, and presentation), d) Developing a research question, analyzing data, and preparing an abstract (individual project), and e) Applying research to grant-writing (a group project). 2) Participate in a longitudinal, community-based study of child health and development – the National Children’s Study. Students will receive the following training components: a) Introduction to the NCS background and methods, b) Participation in community-based research (outreach to potential participants, data collection, entry, and analysis), c) Completion of a paper in the individual student’s area of interest.
REQUIREMENTS TO PARTICIPATE: Interest in pediatrics/child health and development, availability/willingness to make 9-12 month commitment, availability for 8-12 hours per week.
COURSE COMPLETION: N/A
GRADE POINT AVERAGE: 2.5
TIME COMMITMENT PER WEEK: 8-12 hours
NUMBER OF UNITS REQUIRED: 2-3 units (4 hours for each unit)
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Evaluation will be based on performance in three areas: 1) participation in course components (30%), 2) individual abstract/paper (40%), and 3) group project (30%). OFFICE LOCATION: 19722 MacArthur Boulevard, Irvine, CA 92612 AND the Neurodevelopmental Center, 1915 West Orangewood Ave Suite 200, Orange, CA 92868
RESEARCH DESCRIPTION:
The focus of my research laboratory is cell and tissue transplantation with specific focus on the transplantation of pancreatic islet cells for those people with insulin dependent diabetes (type I). Over the past few years there have seen significant and important advances in the method of human islet isolation allowing the transplantation of isolated islets into patients with brittle longstanding diabetes. Our goal is to develop a clinical islet transplant program at the University of California, Irvine. Additionally, we have a active research program investigating aspects of islet isolation including enzymatic means of tissue disassociation, examining cell tissue energetics and novel methods of validating islet yield and function. To address the key issue of tissue rejection and the need for chronic anti-rejection drugs, we are working with an industrial partner on developing a clinical islet encapsulation program using biocompatible alginate. It is hoped that these pre-clinical transplants and biochemical studies will lead to clinical trials in patients with diabetes.
REQUIREMENTS TO PARTICIPATE:
Requirements - General background in biology/biochemistry/immunology. Strong motivation, enthusiasm, good organizational skills, quick study and receptive to taking work direction and working in a team environment. Presentation at scientific meetings along with submission of findings to scientific journals will be encouraged.
COURSE COMPLETION: Completion of core through Bio 99 with a grade of B or higher, completion of Bio 194S Safety, consent of instructor.
GRADE POINT AVERAGE: GPA 3.0 or higher
TIME COMMITMENT PER WEEK: Minimum 15-20 hours per week.
NUMBER OF UNITS REQUIRED: 4. OFFICE LOCATION: UCIMC Bldg 55 Room 160
Role of proteoglycans in mouse brain development. This research is to study the development of the mammalian brain by analyzing mutant mice. The lab has made mutants that lack a proteoglycan, glypican – 1, and which are deficient in brain growth. The goal is to determine what cellular signals are disrupted by this mutation. Methods will include tissue histology, molecular biology, and mouse genetics. Learning how proteoglycans affect brain development in mice will ultimately contribute to the understanding of human birth defects and diseases. Requirements: minimum of 10 hours per week, has a GPA of at least 3.4, preferably has junior standing (but will consider sophomores with excellent grades), and is interested in continuation of this project in future quarters.
RESEARCH DESCRIPTION: We are developing new diagnostic tests that can identify subjects with prostate cancer before they develop clinically obvious disease. Saliva and plasma collected from normal males (no evidence of prostate disease) and from men having prostate cancer will be used for isolation of RNA. RNA will be subjected to RT-PCR, qPCR, and microarray analysis in an attempt to identify and compare types of RNA molecules (derived from different genes) in the saliva and plasma from men with or without prostate cancer. Identification of unique kinds of RNA molecules in the blood or saliva of prostate cancer subjects may allow us to develop diagnostic tests to identify prostate cancer without needing a biopsy.
REQUIREMENTS TO PARTICIPATE: Completion of Bio. 194 Safety & Ethics course prior to enrollment. General background in biology/biochemistry/molecular biology and/or oncology. Motivation and interest in research. Prior lab experience required. Knowledge and experience in molecular biology techniques preferred.
COURSE COMPLETION:
GRADE POINT AVERAGE: 3.0 +
TIME COMMITMENT PER WEEK: 10-12 hrs
NUMBER OF UNITS REQUIRED: 2-3 units (4hrs/unit)
OTHER: Students will be involved not only in generating laboratory data, but also in literature reviews and laboratory presentations. Substantial contribution may lead to paper co-authorship.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Commitment and enthusiasm in finishing assignments and written reports. OFFICE LOCATION: Bldg 56 Rm 248 Rt 81
RESEARCH DESCRIPTION: My work is focused on understanding how radiation-induced changes in redox state impact genomic instability, cell survival, and stem cell fates in tissues from the brain, bone and skeletal muscle. My laboratory has shown that irradiation leads to a myriad of cellular changes, but perhaps the most intriguing are the changes to redox state that can lead to oxidative stress. Radiation-induced oxidative stress varies in magnitude and duration, and is generally more dose responsive at earlier post-irradiation times. In multipotent neural stem and progenitor cells, we have shown that sparsely and densely ionizing radiations elicit an acute and chronic oxidative stress. Reactive oxygen (ROS) and nitrogen (RNS) species are involved and can be linked to changes at the mitochondrial level as well as membrane based oxidases. Oxidative stress in neural cells exhibits both adaptive and bystander responses, and is responsible for altering lineage development and radiosensitivity. In neural (as well as many other cell types) we have shown that chronic oxidative stress can be linked to increased apoptosis, and genomic instability involving a wide range of aberrant phenotypes. Mechanistic links between oxidative stress and cancer are becoming increasingly evident, as is the role of reactive species dictating the repair and remodeling of irradiated normal tissue. Other work is involved with directing the fate of multipotent cells (including hESCs) in specific microenvironments, and how that can be further manipulated through genetic and pharmacologic interventions.
REQUIREMENTS TO PARTICIPATE: Undergraduate major in natural/physical sciences, Interview, 1 yr commitment
COURSE COMPLETION: Certain upper division coursework (flexible)
GRADE POINT AVERAGE: ~3.5
TIME COMMITMENT PER WEEK: 12-24h (12h minimum)
NUMBER OF UNITS REQUIRED: 3-5
OTHER: Motivated, willingness to learn and work hard
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
1) Research results
-Data quality
-Productivity
2) Ability to communicate (oral)
-Data presentation
-Knowledge and scope of research project
3) Ability to communicate (written)
-Preparation of methodologies and resultslts. OFFICE LOCATION: Medical Sciences B (811 on campus map), Room B149
Investigates the neurophysiological mechanisms involved in autonomic reflexes including cardiovascular and acupuncture related mechanisms. Also studying the role of mechanical factors and metabolic products such as bradykinin in stimulating ischemically and reperfusion-sensitive cardiac sensory nerves. These nerves are responsible for angina and a number of other reflexes that lead to hypertension in the immediate post-bypass period, arrhythmias, hypotension, nausea and vomiting. Upper division students who have had introductory physiology/neurophysiology course; 3 quarter commitment. However, year 2 Bio. Majors considered. Students will be involved in whole-animal surgical procedures and histological procedures prefer students who can commit to at least 2-3 hour time blocks. At lest 6 hours/wk. GPA 3.0.
Shahram Lotfipour, M.D.,M.P.H. Emergency MedicineEmergency Medicine
Emergency Medicine Research Associates Program (EMRAP)- The EMRAP program created in 1997 by Dr. Federico Vaca is focused on facilitating clinical research in the Department of Emergency Medicine (EM) at the University of California Irvine Medical Center (UCIMC) in Orange. EMRAP provides undergraduate students first hand exposure to clinical research in the active Level 1 Trauma Center Emergency Department. They will learn to enroll patients in clinical research as well as become very familiar with research methodology. In addition to research exposure, students receive weekly clinical case and topic discussions as well as quarterly procedure workshops. The unique blend of first-hand clinical experience and scientific research has given EMRAP alumni a unique advantage when applying to medical (allopathic and osteopathic), dental, pharmacy, physician assistant and public health programs around the country. While participating in EMRAP, research associates have an extensive opportunity to interface, inform and educate, and dialogue with patients regarding any one of a number of exciting clinical research studies underway in the Department. As a result, research associates become well versed in the workings of clinical emergency medicine as well as patient communication and education. To optimize the research associate experience, students have the opportunity to be more involved with a particular research project and are closely linked with EM faculty and EM residents researchers as a research “liaison” from the onset of the given research project. Every EMRAP research associate plays a vital part in the implementation and maintaining the integrity of research protocols. Requirements include mandatory Monday night meetings at the UCIMC from 7-8:30 PM, 4 hours/unit per week (3 units) and a minimum of one- year commitment to the program (including the summer). Post graduates students are not able to be considered except for UCI School of Medicine post-baccalaureate students. The completed online application is necessary prior to consideration. Interested Spanish speakers are encouraged to email the student coordinators as well as completing the online application. Minimum GPA is 3.0. Please email any questions to our two student coordinators: Rex Chang and Bhakti Patel at emrapcoords@gmail.com. For more information and the online application, please visit our EMRAP website.
EMRAP Director: Dr. Shahram Lotfipour
Research location: the Emergency Department at UCIMC in Orange
Contact:
Rex Chang and Bhakti Patel: EMRAPcoords@gmail.com
RESEARCH DESCRIPTION:
Project 1: Development, Aging and Dementia in Down Syndrome: The objective of this project is to understand the relationship between Alzheimer disease, dementia and Down syndrome. The research is supported by the NIH. Students will have the opportunity to participate in clinical research trials and will have the opportunity for direct patient contact. Students will also assist in database management and will be trained to administer several neuropsychological tests. Students with a major in the biological sciences and a strong interest in clinical research are preferred. There is potential for independent research projects and publication. Contact Eric Doran, M.S.; edoran@uci.edu or (714) 456-8443.
Project 2: Developmental Disabilities and Psychoactive Medications: This objective of this project is to assess the use of psychoactive medications in a community based population of individuals with developmental disabilities. The project is supported by the State of California, Dept of Developmental Disabilities. The assessment process consists of: 1) Evaluation of a computerized pharmacy database for consumers living under the supervision of the Regional Center of Orange County; 2) An interdisciplinary clinical team to evaluate consumers who have severe neurobehavioral disorders and are receiving polypharmacy. The student will assist in database management and will be trained to administer some psychological tests assessing behavior and adaptive functioning. Contact: Christy Hom, Ph.D.: homc@uci.edu or (714) 456-2927.
REQUIREMENTS TO PARTICIPATE: Must be reliable, self-motivated and enthusiastic
COURSE COMPLETION: Commitment of 1 academic year is preferred
GRADE POINT AVERAGE: 3.0 or better
TIME COMMITMENT PER WEEK: Variable
NUMBER OF UNITS REQUIRED: Variable
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of work performed and participation
RESEARCH DESCRIPTION:
Research in my laboratory centers on the mechanisms by which chemical toxicants damage the ovary, potentially causing infertility and ovarian cancer, and in understanding differences in ovarian susceptibility to toxicants. We are particularly interested in oxidative stress as a mechanism of ovarian injury and in the modulation of susceptibility to ovarian injury by biotransformation enzymes and antioxidants.
Many known ovarian toxicants are conjugated by the tripeptide glutathione (GSH), and GSH is also a critical detoxification mechanism for reactive oxygen species. Work in my laboratory has demonstrated roles for oxidative stress in mediating both spontaneous and toxicant-induced apoptosis in granulosa cells of ovarian follicles. We showed that biochemical depletion of GSH significantly increased apoptotic follicles in the ovaries of adult rats. We also showed that GSH depletion in cultured follicles reversed the protective, suppressive effect of follicle stimulating hormone on reactive oxygen species and on apoptosis. Our work suggests that the protective effects of follicle stimulating hormone are mediated in part via upregulation of GSH synthesis. Using cultured follicles and granulosa cells, we have shown that increased generation of reactive oxygen species is an early event in the induction of apoptosis by polycyclic aromatic hydrocarbons and by the anticancer drug cyclophosphamide and that depletion of GSH sensitizes to these agents. Ongoing studies are using genetically modified mouse models with specific deficits in GSH synthesis or with more globally decreased antioxidant and biotransformation capacity to understand the roles these play in ovarian susceptibility to toxicant-induced ovarian damage and ovarian cancer.
REQUIREMENTS TO PARTICIPATE: Motivation, enthusiasm. Sophomore or Junior, 3.5 GPA, minimum commitment of 1 academic year, 2 years preferred, 10-15 hours per week minimum, willing to work occasional weekends, willing to work with mice, attend weekly lab meetings and present research in lab meetings.
GRADE POINT AVERAGE: 3.5 or better
TIME COMMITMENT PER WEEK: 10-15 hours
NUMBER OF UNITS REQUIRED: 3 to 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Presentations in lab meetings, understanding of topic, reliability, effort put into work. OFFICE LOCATION: 5201 California Avenue, Suite 100
http://bass.bio.uci.edu/~hudel/
The main focus of my laboratory is the structure-function investigation of integral membrane proteins . To date the atomic structures of less than 50 membrane proteins are known (vs. over 20,000 for soluble proteins). This is in stark contrast to the fact the most genomes contain 20-30% membrane proteins. Recently, we have solved the atomic resolution structure of the light-driven ion pump bacteriorhodopsin (BR) in the resting state at very high (1.55 resolution. Together with the structures of several photocycle intermediates "frozen in mid-stroke" we have been able to develop a detailed atomic mechanism of light-driven ion pumping. In addition, the structures of a related membrane protein that serves as the primary receptor in archaeal phototaxis (sensory rhodopsin) and that of a photoreceptor from Anabaena , the first eubacterial rhodopsin structure, have been determined. We are also studying annexins , a family of proteins which interact with phospholipid bilayers in a Ca2+-dependent manner. Annexins have been reported to mediate membrane aggregation and fusion, as well as ion channel formation. Detailed structural studies of annexins are essential for understanding their fusogenic and ion channel forming properties at the atomic level. Another area of interest is structure-based drug design . We have recently solved the structure of a key enzyme in purine metabolism, inosine-5'-monophosphate dehydrogenase (IMPDH). IMPDH catalyzes the NAD-dependent conversion of IMP to XMP which in turn is converted to GMP, an essential building block of DNA. The IMPDH-reaction is the rate-limiting step in GMP synthesis and is thus a promising target for anti-parasitic and anti-bacterial drugs. We are currently focusing on IMPDHs from P. falciparum , M. tuberculosis , and T. foetus p53, the well-known tumor suppressor Nuclear receptors, in particular PPAR-alpha, involved in obesity. A further interest of my group are very high resolution (1.0 or better) structures of phosphate binding protein. These studies require synchrotron-generated X-rays of very high brilliance. Atomic structures at this resolution are able to reveal details of hydrogen bonding that cannot be obtained by other methods. See also http://bass.bio.uci.edu/~hudel/ Due to the techniques we use we require a long-term commitment (ideally 2 years). Most projects will include setting up crystallization trials - often resulting in a paper if diffracting crystals are obtained.
Gene Regulation & intracellular signaling pathways contributing to pain transduction. Use of animal models mimicking human pain inducting conditions, such as nerve injury and bone cancer, to identify potential target genes and pathways, and study their regulation in sensory neurons and spinal cord and their relationship to pain development under these pathological conditions. Small animal surgery, behavioral pharmacology, cellular and molecular biology techniques, including fluorescent microscopy, gene chip experiments, and transgenic/knock out mice, are utilized in studies. Currently characterizing several genes and signaling pathways that may play important roles in neuropathic pain (pain derived from nerve injury) and cancer pain processing. Required: completion of basic courses of biology, cellular and molecular biology or animal physiology or computer science with a GPA of B or higher, three letters of recommendation. At least 1 year commitment. Selection: academic achievements, experience, enthusiasm, self-motivation.
Gary Lynch, Ph.D. Psychiatry and Human Behavior
Ongoing projects in the laboratory focus on neurodegenerative diseases, especially Huntingtons Disease (HD), and aging. We are also studying the effects of modulating the AMPA-type glutamate receptor on sexual behavior. Specific projects include: (1) Role of Inflammation and Iron in HD Pathogenesis: Brains of HD patients and HD mutant mice have increased reactive microglia that appear dystrophic and contain iron very early in the disease. This study will further characterize the inflammatory response in HD brains and HD mutant mice and determine if chelating iron, inhibiting microglia or both decreases inflammation and/or neurodegeneration in cultured corticostriatal slices from HD mutant mice and in vivo. Bio 199 students will be involved in: performing immunocytochemistry, western blotting, and behavior tests for motor performance. (2) Role of Brain Derived Neurotrophic Factor in the Cognitive Deficits Associated with HD: The goal of the project is to elucidate the role of Brain Derived Neurotrophic Factor (BDNF) in cognitive deficits associated with HD. Aims to determine: the time course of electrophysiological deficits in HD knock in mice; if the deficits in synaptic plasticity correlate with altered BDNF levels and/or cytoskeletal changes. Bio 199 students will be involved in: performing immunocytochemistry and western blotting. (3) Effects of AMPAkines on Sexual Behavior: This project will investigate the effect of AMPA-tpye glutamate receptor modulators, AMPAkines, on male and female sexual behavior. This project will identify AMPAkines that may be effective in increasing sexual motivation and/or alleviating sexual dysfunction. Roles for 199 students: Performing behavior tests for rat sexual behavior and performing immunocytochemistry. (4) Effects of Aging on Cytoskeletal Changes that occur with Learning and Memory: Changes in the shape of dendritic spines occur when animals learn. This shape change is triggered by long-term potentiation (LTP), which is thought to be the way memory is encoded in the brain. During LTP, the cytoskeleton rearranges itself and levels of the filamentous (F-) actin protein are increased. We will look to see if F-actin is increased in brain areas involved in learning andd memoryafter rats perform a learning task. Also aged animals that show decreased learning and memory should have less F-actin than young rats. Roles for 199 students: performing rat surgeries to implant cannulas, and performing behavior tests for learning. Requirements: commitment of at least one year, desire to work with live animals, available 9 hours a week or more.
Shaista Malik, MD, PhDDepartment of Medicine, Division of Cardiology
RESEARCH DESCRIPTION:
The focus of our research is the role of cardiovascular imaging, such as cardiac CT, in preventive cardiology. We have a current clinical trial in examining the effects of cardiac CT in identifying early coronary artery disease in patients with diabetes. We are also involved in retrospective data analysis on relationship between cardiac plaque on CT and patient risk factors.
REQUIREMENTS TO PARTICIPATE: Have completed UCI IRB tutorials on human research and HIPAA
TIME COMMITMENT PER WEEK: 3-4 hours a week for each unit of course credit.
NUMBER OF UNITS REQUIRED: 2-4 units. LOCATION OF RESEARCH: UCI medical center
Darren Malinoski, M.D.Surgery, Division of Trauma/Critical Care/Burns
Trauma Research Associates Program (T-RAP) – The T-RAP is a newly created program that is focused on facilitating clinical research in the Department of Surgery’s Division of Trauma/Critical Care/Burns at the University of California Irvine Medical Center (UCIMC) in Orange. T-RAP provides undergraduate students first hand exposure to clinical research in Orange County’s only Level-1 Trauma and Burn Center. The T-RAP experience involves a unique blend of first-hand clinical exposure and scientific research, which should serve useful when applying to medical (allopathic and osteopathic), dental, pharmacy, physician assistant and public health programs around the country. Research associates become well versed in the workings of trauma surgery and critical care as well as patient communication and education. Provides opportunities to watch surgical procedures and attend medical rounds with doctors. Students will be required to attend mandatory Friday meetings at the UCIMC from 12:30-2 PM. Other requirements include: 4 hours/unit per week (3-4 units), a minimum of a one-year commitment to the program (including the summer), and a GPA of >/= 3.0. Email any questions to Dr. Malinoski at malinosd@uci.edu and/or the program coordinator Fariba Jafari at fjafari@uci.edu. To apply for a position please fill out an application at website listed below:
Research in Dr. Marshall’s lab is directed at (1) the neurobiological bases of chronic drug abuse (cocaine, amphetamine), and (2) factors that facilitate outcome after brain injury relevant to Parkinson’s disease. Both projects use animal models to study brain events contributing to human disease. Undergraduates participate in many ways, including handling and training animals, sectioning and staining tissue, analyzing tissue, and assisting in neurosurgical procedures. Students participating for more than 2 quarters are encouraged to develop projects in which they take more of a lead role.REQUIREMENTS TO PARTICIPATE: Coursework (Bio Sci 110), GPA, motivation to do research, communication skills.
COURSE COMPLETION: Bio Sci 110. 15 hours/week. REQUIRED: 9 units. GPA 3.5. Readings will be assigned to supplement laboratory work. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of research, understanding of topic, reliability
Adam Martiny, Ecology & Evolutionary Biology, Earth System Science
RESEARCH DESCRIPTION: My lab works at the interface between microbiology, ocean ecosystem ecology, and global change. In particular, we are interested in understanding the present and future global distribution of marine cyanobacteria and how they will respond to ocean warming. This includes how cyanobacteria will evolve and adapt to new environments. Student projects could include lab work using culturing and molecular techniques (e.g. PCR), DNA sequence analysis, and modeling.
REQUIREMENTS TO PARTICIPATE: Students should be prepared to work with other group member as well as develop independent projects and spend at least 10 hours per week on research. A multi-quarter commitment is strongly preferred, and students will be expected to participate in lab activities and meetings.
COURSE COMPLETION: no requirement
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 10
NUMBER OF UNITS REQUIRED: 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Grades will be based on time committed to the project and the quality of research. OFFICE LOCATION: 3208 Croul Hall
Jennifer Martiny, Ecology and Evolutionary Biology
RESEARCH DESCRIPTION:
Our lab investigates the mechanisms underlying microbial diversity patterns and how this diversity affects ecosystem processes such as nutrient cycling. We use culturing and molecular genetics tools to quantify the diversity of microorganisms including bacteria, archaea, fungi, and viruses in a variety of systems such as grasslands and the coastal ocean. Undergraduate projects mostly involve laboratory work, but opportunities exist for fieldwork and computer modeling.
REQUIREMENTS TO PARTICIPATE: Bio 194S Saftey & Ethics
COURSE COMPLETION: none
GRADE POINT AVERAGE: 3.0 minimum
TIME COMMITMENT PER WEEK: at least 12 hours
NUMBER OF UNITS REQUIRED: 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: proposal, weekly meetings, time commitment and quality of research, research report. OFFICE LOCATION: Steinhaus 455
Assessment of new vehicles as topical medications, development of methods for delivering drugs through skin. Percutaneous absorption testing using Franz Diffusion Chambers to assess migration of pharmaceuticals through the integument, and analysis of the distribution in skin layers. Use of liquid scintillation chromatography for quantitation of drug penetration. Occasional use of in vivo models for assessment of new drugs and vehicles. Students are assessed each quarter in three areas 1)attendance, 2)participation, 3)quarter thesis (topic arranged each quarter); continuous participation for 3 quarters confers eligibility in BioMed 199 Excellence in Research Program. Min. 12 hrs/wk. 3-4 units. GPA 3.0.
Endourology lab. Multiple large animal surgical experiments as well as other “bench top” experiments in progress on an ongoing basis. Usually the student is assigned a project as primary research and also participates in all of the other experiments as well. Part of the job involves setting up and breaking down the lab for animal experiments and working with the animals (pigs, rabbits) directly after appropriate training. The level of responsibility is graded depending on the experience level of the student and increases as time goes on.
A.) Tissue bioengineering: bladder replacement
b.) Prosthetics and other surgical materials: bladder replacement, stent development
c.) New technology for laparoscopic surgery
d.) Studies on endoscopes
e.) Clinical research into laparoscopic surgery outcomes in Urology
Science back ground and interest in surgical research. No prerequisites. 4-10 hours/wk. GPA 3.25 or above.
RESEARCH DESCRIPTION:
Our lab is involved in studying the study of gene regulation by transcription factors important in cancer using high throughput methods. One example is the role of immediate early genes in prostate and breast cancer. Early growth response 1 (Egr1) is a transcription factor that is over-expressed in prostate cancer. Our goal is to understand the role of Egr1 in prostate cancer. We have done ChIP on chip (promoter array) studies to identify targets of Egr1 in UV irradiated prostate cells. The results show that various Egr1 mediates the coordinated expression of gene down stream of the EGF receptor and includes genes involved in apoptosis, and cell cycle regulation, as well as from the cytokine family. Genes regulated by Egr1 were validated using conventional immunoprecipitation, gene expression and SiRNA studies. The 199 research will consist of studying some of these target genes and their role in stress response and response to EGF. The project will involve extraction and purification of total protein and RNA from prostate cancer cells after treating these cells with various stress stimuli. Then the expression levels of individual proteins and gene transcripts in these samples will be measured by western blotting and real time (quantitative) RT-PCR using highthroughput methods. A final aspect of the project is to summarize and prepare the results.
REQUIREMENTS TO PARTICIPATE: provide resume including all summer and other employment and volunterr activity to D. Mercola
COURSE COMPLETION: Presentation at term end at a regular laboratory meeting.
GRADE POINT AVERAGE: G.P.A. of 3.0+.
TIME COMMITMENT PER WEEK: 12 hours/week per quarter
NUMBER OF UNITS REQUIRED: Four
OTHER: Other projects suitable for 199 participation include chip-on-chip analysis of transcription factor regulation in cancer using lab-developed and commercial promoter arrays, analysis of mechanism of Herceptin action in breast cancer, and bioinformatics analysis of cell-specific gene expression in prostate cancer.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Term end presentation, lab notebook completeness, participation as coauthor in lab study if any, attendance record. OFFICE LOCATION: C-238, Med Sci1
Frank Meyskens, Jr., M.D.,F.A.C.P.Comprehensive Cancer Center
My laboratory investigates the biology of human melanoma and the factors that contribute to its etiology and progression
Current work is focused on redox-responsive transcription factors and the contribution of heavy metals
3units 12hrs/week GPA 3.0 Experince with cell culture desireable
Research opportunity is available in a medical imaging laboratory in the area of cardiovascular imaging. The learning opportunities include image and data acquisition, image processing, 3-D reconstruction and data analysis techniques. You can also participate in the development of our new medical imaging devices. Students can also be involved in whole-animal surgical procedures. Some background in hardware or software and a strong motivation to learn and participate in research projects are essential. Knowledge of programming languages such as Java, C++ is an asset.
Forebrain development with focus on the molecular and cellular basis of cerebral cortex and choroid plexus development, including models of human birth defects. Experimental systems involve mouse genetics,explant and dissociated cell cultures, including work on mouse and human neural stem cells. Stem cell studies also include traditional and microfluidic cultures in collaboration with UCI biomedical engineers. Requirements to participate: Drive, motivation, enthusiasm, interest, and intellect. Team player, but desires independent project. Attends and presents at lab meetings. Minimum 1.5 year commitment, including summers. Biology and/or chemistry wet lab experience
required. Previous lab experience preferred. Top 25% among peers. Minimum 10 hours/wk during school year; 20 hours/wk during summer.
RESEARCH DESCRIPTION:
We use field and laboratory studies to investigate the interactions among insects and spiders, how insects affect plants and in turn how plants affect insects. Our research employs a variety of techniques, including growing of plants and maintenance of insect colonies in the lab and greenhouse, measurement of plant chemical and morphological traits, and sampling and quantification of insect communities in the field. We are primarily interested in students willing to make a multi-quarter commitment. Students who make long-term commitments may have opportunities for summer fieldwork at remote field sites and for co-authorship on a scientific research paper.
REQUIREMENTS TO PARTICIPATE: Students should be prepared to develop independent projects and spend at least 10 hours per week on research. Students will be expected to participate in lab activities and meetings.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: grades will be based on time committed to the project and the quality of research. OFFICE LOCATION: 421 Steinhaus
Jorgesh Mukherjee, Ph.D.Psychiatry & Human Behavior
Design, synthesis, and use of novel imaging agents. Targets include various enzymes, receptors and neurotransmitter receptor systems, such as dopamine, serotonin, norepinephrine, acetylcholine, glutamate and others. Applications of imaging agents include use in diagnosis of various pathophysiological conditions, evaluate effects of therapeutic drugs, effects of substance abuse drugs, study in vivo biochemistry for eventual use in human studies.
Edward L. Nelson, M.D.Department of Medicne, Hematology/Oncology
Dr. Nelson conducts research into mechanisms for eliciting antigen specific, anti-tumor immune responses and methods for translation into clinical trials. Specifically, he is developing strategies for individualized polyvalent tumor-specific immunotherapy that take advantage of immune system dendritic cell biology. Dr. Nelson is one of only a handful of investigators developing anti-tumor immunotherapy strategies using vectors derived from alphaviruses, specifically the VEE replicon system. These replicons exhibit in vivo and in vitro tropism for a subset of professional antigen presenting cells, i.e. dendritic cells. Dr. Nelson has demonstrated very promising results for this immunotherapy targeting a non-mutated tumor associated antigen, the homologue of human Her 2/neu, in an animal model of breast cancer. Dr. Nelson is also investigating “naked DNA” or polynucleotide vaccine (PNV) immunization strategies using a unique plasmid vector designed for anti-tumor immunotherapy applications. Dr. Nelson is principle investigator on several clinical trials of anti-tumor immunotherapy. These trials include studies of the growth factor GM-CSF, which is a requisite trophic factor for dendritic cells, as a biological adjuvant and the additive effect of immunostimulatory DNA with antibody directed therapies, i.e. Herceptin.
Research: Students will work on projects associated with assessing the effectiveness of specific teaching strategies, both in terms of performance and attitude of undergraduate students, graduate students, and faculty. Students will be involved in formulating hypotheses, designing experiments to be conducted during teaching, and analyzing data we gathered during the previous quarters. Requirements: B or higher in Bio 93 and are able to commit 10 hrs/week.
Description: Students will be work with patient who have Inflammatory Bowel Disease. The experience will span the spectrum of clinical research, including project design, background literature search, and help recent research or assist in writing an IRB (institutional review board) proposal, enrolling patients in ongoing studies, data compilation or writing/presenting research. Experience as a research associate will be enhanced by being assigned to faculty and staff as liaisons for each project. Wednesday afternoon research meetings will help to monitor progress as well as assist in furthering your research experience. Opportunities for publication and possibly presentation at local and national conferences are available. Requirements would include:
-3.5 overall GPA
-sophomore standing
-one to two year commitment
-Four hours/unit/wk
-Minimum of 2 units/4 units with the option for more units upon request
-Ability to work independently as well as in a group setting
Students will be graded on:
-Work performance
-Initiative
-Quarterly research summary
Location: UCI Medical Center and at Gottschalk Plaza
We are applying advanced imagin methods (confocal, multi-photon and evanescent wave mivroscopy) to study intracelllular calcium signaling processes. Ion channels are mediate the functioning of all cells, including processes such as synaptic transmission and electrical excitation. Moreover, they are implicated in many disease such as schizophrenia, Alzheimer's diseases, and Parkinson's, making them important targets for drug therapies. Our laboratory is currently involved in developing new imaging techniques that permit us to visualize the activity of individual ion channels in living cells – something previously possible only by the electrophysiological patch-clamp technique. Positions are available for 2 students to be involved in different aspects of the project. (1) Expression of ion channels in Xenopus laevis oocytes . This will involve learning basic molecular biology techniques such as cell transformation, propagation, in-vitro transcription and gel electrophoresis. (2) Analysis of single channel activity using image analysis and graphing software. Requirements: Sophomore (in exceptional cases) or Junior standing, 3.2 GPA (this is sometimes negotiable), minimum commitment 1 year (3 quarters), 2 years preferred; 8-12 hours per week.
Research positions are available to work on a number of projects related to the effects of urbanization on plant communities, and the effects of vegetation on the urban environment. rojects include studies of: greenhouse gas emissions from turfgrass at the UCI Arboretum; vegetation change in Owens Valley, CA; water fluxes in urban environments; and measurements of the isotopic composition of plant, soil, and air samples collected throughout the Los Angeles Basin. inimum requirements are a one year commitment and at least 3 units (9 hours) per quarter.
The psychoactive effects of 9-tetrahydrocannabinol, the major pharmacological ingredient of Cannabis sativa, are caused by the activation of G protein-coupled membrane receptors called cannabinoid receptors. The expression of these receptors __ abundant in brain structures involved in cognition, emotion and movement __ and the behavioral consequences of their activation __ ranging in man from euphoria to memory deficits __ underscore the potential importance of the endogenous signaling system by which these receptors are thought to be engaged. Yet, the biochemical nature, anatomical distribution and physiological functions of such signaling system remain largely unknown.
Understanding the biochemistry and pharmacology of the body’s own cannabinoid system is the major long-term objective of the research in our lab. To progress in this direction, we adopted two complementary approaches. On the one hand, we use biochemical and molecular biological techniques to study the mechanisms of formation and inactivation of endocannabinoid substances. On the other hand, we apply this biochemical information to design drugs that interfere with endocannabinoid metabolism.
The development of pharmacological agents that prevent formation or inactivation of the endocannabinoids is essential to understand the physiological functions of these signaling molecules. Such drugs may not only prove to be useful experimental tools, but in light of the multiple behavioral effects of cannabinoid receptor activation might also open novel therapeutic avenues for the treatment of neurological and psychiatric disorders. REQUIREMENTS TO PARTICIPATE: Willingness to learn and excitement about science. 9-12 hours/wk. REQUIRED: 3 units. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of work performed and participation.
Current research involves numerous structural and functional MRI, PET, EEG and other imaging studies. Students will be involved in data collection, analysis and visualization, with weekly team meetings to discuss the current projects and interpret results. Looking for research students interested in learning more about brain imaging, brain function and dysfunction. Must have 1 year commitment minimum and 3.0 GPA.
Our lab goals are to faster research into the role of the mitochondrion in disease and neurodegenerative disorders and to use this knowledge to develop more effective diagnostics and therapeutics. Mitochondria are organelles responsible for the production of most of the energy needed described as ‘the powerplant of the cell'. Our program coordinates clinical and basic research as well on the role of mitochondrial defects in a spectrum of disorders and successful students will have the opportunity to participate in research projects covering all aspects of mitochondrial research. Students will be trained in advanced molecular biological techniques including tissue culture, DNA and RNA isolation, cDNA synthesis, molecular cloning, PCR, sequencing, mitochondrial biochemical analysis, Western blotting, gene expression array and other techniques needed for the research project. Requirements: major in biological sciences, GPA 3.0 or higher, sophomore preferred, one year commitment or more.
Contact:
Vincent Procaccio: vproca@uci.edu, (949) 824-8092
Yufen Qin, M.D., Ph.D.Neurology
The goal of our research is to characterize the cellular and molecular immune mechanisms that lead to demyelination and axon loss in patients with multiple sclerosis. We expect that elucidation of these pathological mechanisms will provide new opportunities to prevent and/or delay neuron and axon degeneration in the central nervous system of affected patients. Office location: Med Sci II, 301A and B. Should work for at least 1 school yr. GPA: 3.5+. 12-15 hrs/wk.
José M. Ranz, Ph.D.Ecology and Evolutionary Biology
RESEARCH DESCRIPTION: Two positions are offered. Our research interests lie at the interface of functional and evolutionary genomics using Drosophila as a model organism. In particular, we are interested in understanding how the molecular organization of the genome is shaped by functional constraints. We have identified a limited number of genomic regions where gene order has been preserved unexpectedly upon the multiple chromosomal rearrangements that have occurred during the evolutionary process. Those regions can encompass up to 50 genes. We have functionally analyzed those regions and found that they are often enriched for genes with similar functional properties. We are using genetic techniques of chromosomal engineering to disrupt those regions and to evaluate the effect of breaking those ultra-conserved regions of the genome using different genetic and molecular approaches. Specifically, we are performing molecular and physiological tests on individuals with both intact and disrupted regions, which will allow us to compare the gene expression and effects on fitness due to the disruption of those regions. Genomic DNA extraction, RNA extraction, PCR, RT-PCR, microarray experiments, tissue dissection, sequence analysis, data mining, and stock maintenance are the most relevant tasks associated with the projects described here.
REQUIREMENTS TO PARTICIPATE 3 quarters minimum
COURSE COMPLETION Bio194S, Bio97, Bio99, and at least one among E106, D145, and D146 is advisable.
GRADE POINT AVERAGE at least 3.2
TIME COMMITMENT PER WEEK: 12 hours minimum
NUMBER OF UNITS REQUIRED: 4 units will be given for 12 hours per week.
OTHER: Students interested in performing research in our lab are encouraged to visit the lab prior to requesting a position (http://ranzlab.bio.uci.edu).
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Professionalism (enthusiasm, effort, attendance, high-quality work, team-work attitude) and quarterly research report. OFFICE LOCATION: 465 Steinhaus Hall
Contact:
José M. Ranz: jranz@uci.edu, (949) 824-9071
Kevin M. Reavis MD; Ninh T. Nguyen MD FACS- Minimally Invasive and Gastrointestinal Surgery
RESEARCH DESCRIPTION: The Division of Gastrointestinal Surgery involves examination of various conditions and treatments affecting diseases of the esophagus, stomach and small intestine along with metabolic/bariatric surgery. Students will be expected to attend weekly clinics on Mondays and Tuesdays, as well as work independently in the office. Students are invited to observe surgery as time permits. Student tasks will include maintaining the research database, statistical analysis, and other research duties as necessary. Ability to learn new applications. Experience with Excel, Access, SPSS, Adobe Premiere, Adobe Photoshop, Adobe Illustrator, Video editing preferred. Must be willing to travel to medical center. Student will be supervised by the PI and the surgical research resident. REQUIREMENTS TO PARTICIPATE: 3.0 overall GPA and sophomore standing COURSE COMPLETION: Bio 194S. Statistics and physiology recommended but not required GRADE POINT AVERAGE: 3.0 TIME COMMITMENT PER WEEK: Approximately 12 hours/week NUMBER OF UNITS REQUIRED: 4 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of work, fulfillment of time commitment OFFICE LOCATION: 333 City Boulevard West, Suite 850, Orange, CA 92868
Contact:Katie Teetor, Kevin M. Reavis MD, Ninh T. Nguyen MD FACS ; 714-456-8598
Rainer K. Reinscheid, Ph.D.Pharmaceutical Sciences
Rainer K. Reinscheid, Ph.D. Pharmaceutical Sciences
We study the function of novel neuropeptide systems in anxiety, arousal and sleep by using molecular biology, pharmacology and animal behavior techniques. Our goal is to identify the function of these transmitter systems in healthy brains and their association with brain disorders, such as stress, sleep disorders, memory impairment, drug addiction, etc. Students will have opportunity to participate in experiments covering all aspects of pharmacological research: molecular (cloning) and cellular (cell culture) biology, pharmacology (receptor binding and second messenger assays) and animal behavior (if desired).
REQUIREMENTS TO PARTICIPATE: students interested in pharmacological/pharmaceutical research; commitment: 2 years or more. Preference to those with experience in biological and/or chemical techniques. GRADE POINT AVERAGE: 3.3+ TIME COMMITMENT PER WEEK: 8-12 hours/week NUMBER OF UNITS REQUIRED: 2 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: participation, willingness to learn, responsibility OFFICE LOCATION: 310C Med Surge II, School of Medicine
Our lab uses experimental evolution and evolutionary physiology as approaches to address basic problems in biology. Fruit flies of the genus Drosophila are our experimental tool. Some of our research topics include the evolution and physiology of aging, cryonic preservation and resuscitation, reverse evolution, and evolutionary convergence in novel environments. We run a large lab in which undergrduates play a central role at every stage of the research process.
Requirements to participate: Biology 96 or 106 with minimum grade of B, or Biology 94 with a minimum grade of A-. 3.0 grade point average, 10 hours per week to start.3 units for beginning students. *All research students start as Bio. 198 group research students. Progress to Bio. 199 status depends on the student's abillity to work independently. NO student begins in the Bio. 199 course.
Faculty Means of Evaluation for Course Grade: For 198: successful completion of hours, work assignments, and attendance at lab meetings; progress in understanding the scientfic background to the research. For 199: development of a successful research plan, execution of research plan, scientific understanding, completion of Excellence in Research requirements.
Contact:
Michael R. Rose,PH.D.: mrrose@uci.edu, (949) 824-8198
Scott Rudkin, M.D.Emergency Medicine
Requirements: 3.5 overall GPA and junior standing. A minimum of one-year commitment of at least 12 hours/week. Emergency Medicine Workforce Issues: Analysis of factors that limit the willingness of on-call physicians to provide federally mandated emergency care. Emergency Department Efficiency: Investigation of barriers that limit the ability of emergency departments to provide timely emergency department care. Arterial Blood Gas vs. Venous Blood Gas Testing in Trauma patients: Determination whether a venous sample can supplant the need for an arterial source for blood gas testing in critically ill patients. SELECTION CRITERA: Interview.
Number of Units/Quarter: Negotiable, based on hour commitment
Examination N/A
Quality of Work XX
Quantity of Work XX
The Ruf lab is interested in defining the contribution of Epstein-Barr Virus, an ubiquitous Herpesvirus, to the development of a number of types of cancer. Projects in the lab are aimed at defining the role of viral gene products in viral latency and their mechanisms of action in the development of tumors. The lab is particularly interested in the role of two small non-coding RNAs made by the virus. Projects for undergraduates will vary but will likely involve basic molecular biology and biochemical techniques as well as mammalian cell culture. Previous students have been included as authors on manuscripts submitted for publication.
REQUIREMENTS TO PARTICIPATE: Biological Sciences Majors, 3 quarter commitment preferred. Highly motivated students with interest in virology and cancer biology.
COURSE COMPLETION: Sophomore, junior, seniors. Bio 98, 99. Lab coursework (genetics, biochemistry, or molecular biology lab) preferred but not essential.
GRADE POINT AVERAGE: >3.0
TIME COMMITMENT PER WEEK: 8-12 hours
NUMBER OF UNITS REQUIRED: 3
OTHER: Attendance at and quarterly presentation of results at weekly lab meetings if course schedule allows.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Effort put forth and time spent in lab. Results obtained and end of quarter summary/report.
RESEARCH DESCRIPTION:
Project 1. Investigating the role of the keratin cytoskeleton. Keratins are a large family of proteins that form the intermediate filament cytoskeleton in epithelial cells. Mutations in keratin genes are the cause of a number of inherited disorders which are characterized by cell fragility and tissue thickening. Intracellular keratin aggregates are a feature of many keratin disorders and cells expressing mutant keratins are prone to keratin aggregate formation following exposure to environmental stresses such as changes in temperature and osmotic pressure. These observations suggest that aberrant protein-folding may contribute to disease phenotype. My lab is studying the effects of hyperosmotic stress on keratinocytes expressing normal and mutant keratins and is investigating the ability of compounds that promote normal protein-folding to ameliorate the effects of mutant keratins. Such compounds may be useful as therapeutic agents for treating keratin disorders.
Project 2. Investigating the role of keratinocytes in wound healing and regeneration. Most vertebrates respond to amputation injury by wound closure rather than regeneration. This processes invariably results in scar formation. The axolotl is an exception to this rule and, in addition to scar free healing, is capable of regenerating apendages including limbs and tails. Regeneration is believed to recapitulate the developmental process and involves epithelial/mesenchymal interactions. My lab is developing cell culture models to investigate the differences axolotl and mammalian wound healing.
Projects will involve tissue culture, molecular biology, protein gel electrophoresis, microscopy, immunocytochemistry. Studendts are also expected to contribute to general lab duties such as reagent preparation, lab maintenance and clean up.
REQUIREMENTS TO PARTICIPATE: Biochemistry and Molecular Biology, Biological Sciences or Developmental and Cell Biology
COURSE COMPLETION: Bio199
GRADE POINT AVERAGE: 3.5
TIME COMMITMENT PER WEEK: 12 to 16 hrs
NUMBER OF UNITS REQUIRED: 3 or 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Quality of work,
Quantity of work,
Written report (quarterly). OFFICE LOCATION: Natural Sciences 2, rm# 5101
Contact:
Elizabeth Rugg: erugg@uci.edu, (949) 824-8811
Elizabeth Rugg, Ph.D.Dermatology
RESEARCH DESCRIPTION:
Project 1. Investigating the role of the keratin cytoskeleton. Keratins are a large family of proteins that form the intermediate filament cytoskeleton in epithelial cells. Mutations in keratin genes are the cause of a number of inherited disorders which are characterized by cell fragility and tissue thickening. Intracellular keratin aggregates are a feature of many keratin disorders and cells expressing mutant keratins are prone to keratin aggregate formation following exposure to environmental stresses such as changes in temperature and osmotic pressure. These observations suggest that aberrant protein-folding may contribute to disease phenotype. My lab is studying the effects of hyperosmotic stress on keratinocytes expressing normal and mutant keratins and is investigating the ability of compounds that promote normal protein-folding to ameliorate the effects of mutant keratins. Such compounds may be useful as therapeutic agents for treating keratin disorders.
Project 2. Investigating the role of keratinocytes in wound healing and regeneration. Most vertebrates respond to amputation injury by wound closure rather than regeneration. This processes invariably results in scar formation. The axolotl is an exception to this rule and, in addition to scar free healing, is capable of regenerating apendages including limbs and tails. Regeneration is believed to recapitulate the developmental process and involves epithelial/mesenchymal interactions. My lab is developing cell culture models to investigate the differences axolotl and mammalian wound healing.
Projects will involve tissue culture, molecular biology, protein gel electrophoresis, microscopy, immunocytochemistry. Students are also expected to contribute to general lab duties such as reagent preparation, lab maintenance and clean up.
REQUIREMENTS TO PARTICIPATE: Biochemistry and Molecular Biology, Biological Sciences or Developmental and Cell Biology
COURSE COMPLETION: Bio199
GRADE POINT AVERAGE: 3.5
TIME COMMITMENT PER WEEK: 12 to 16 hrs
NUMBER OF UNITS REQUIRED: 3 or 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Quality of work,
Quantity of work,
Written report (quarterly). OFFICE LOCATION: Natural Sciences II, room # 4131
Contact:
Elizabeth Rugg: erugg@uci.edu, (949) 824-8811
Michael Rugg, Ph.D.Neurobiology
Behavioral EEG and fMRI study of encoding and retrieval operations in human memory.
Requirements to participate:>3.5 grade point average, minimum of 6 hours per week.
Faculty means of evaluation for course grade: lab work and term paper.
Contact:
Michael Rugg: mrugg@uci.edu, (949) 824 – 8531
Hamid M. Said, Ph. D., Pharm. DMedicine, Professor and Vice-Chairman for Research.
RESEARCH DESCRIPTION: To study cell and molecular mechanisms involved in the transport of water-soluble vitamins (thiamin, riboflavin, folate, biotin, pantothenic acid, niacin, pyridoxine) in intestinal/renal epithelial cells and in pancreatic beta and acinar cells in health and disease. We also examine regulation of vitamin transport and the effect of chronic alcohol consumption, congenital defects in the transport protein, drug-interactions and effect of dietary factors on vitamin transport processes. Our laboratory is funded by grants from the NIH and other national agencies. Direct involvement of the students in the research projects is expected.
REQUIREMENTS TO PARTICIPATE: The student should have completed biology, biochemistry or a related class
COURSE COMPLETION: Year round
GRADE POINT AVERAGE: No less than 3.0
TIME COMMITMENT PER WEEK: A total of 14-16 hrs per week
NUMBER OF UNITS REQUIRED: 3 to 4
OTHER: N/A
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance and actual contribution. LOCATION OF RESEARCH: Long Beach VA Medical Center.
Ann K. Sakai, Ph.D.Ecology and Evolutionary Biology
RESEARCH DESCRIPTION: Flowering plants have a great diversity of breeding systems. Some species are hermaphroditic, others have females and hermaphrodites, and still others have only males and females. Our lab is interested in factors that promote these different breeding systems and the genetic potential of plants to evolve these different breeding systems. Understanding these processes provides information important in conservation, agriculture, and basic sciences. Undergraduates are directly involved in research and work closely with faculty and students in the greenhouse and laboratory on the rare Hawaiian genus Schiedea and on the genus Oxalis. For further information, check out our website (see below).
REQUIREMENTS TO PARTICIPATE: Interest in ecology, conservation and/or evolution; prefer students with more than one year remaining at
UCI COURSE COMPLETION: Bio 94
GRADE POINT AVERAGE: prefer GPA of 3.0 or higher
TIME COMMITMENT PER WEEK: variable but must be willing to work some weekends.
NUMBER OF UNITS REQUIRED: variable; minimum of 2 units per quarter
OTHER: Most of our students participate in the Excellence in Research program. OFFICE LOCATION: 365 Steinhaus Hall
Evaluating the consequences of prenatal exposure to stress and stress hormones for fetal and infant development. Students working on this project will have the opportunity to work with doctors, nurses, infants and parents. Research assistants collect data at UCI pediatric clinics and at the NICU and newborn nursery at the UCI Medical Center . Spanish speaking students have the opportunity to conduct interviews in Spanish. We are looking for highly motivated students with an interest in medicine, psychology, basic biology, or public health, who are willing to work 10-12 hours/week for a minimum of 1 year. Training will be provided. Students should have access to transportation to and from the Med Center .
GPA should be 3.3 or higher. Science majors. Completion of introductory biology and general chemistry. Genetics, biochemistry or molecular biology lab experience preferred but not essential. Summer: 20 hours per week. Fall: 10-15 hours per week. Prefer one year commitment. Laboratory works on Ty3 which is a retrovirus like element in bakers yeast. Retroviruses are important because they are the causative agent of AIDS and because they are used as gene therapy vectors. They are unique in that they reverse transcribe an RNA genome into DNA and then integrate into the chromosomal DNA. The long term goal of this work is to identify homologues of proteins which are important for retrovirus replication in mammalian cells. These would be potential targets of antiretrovirus therapy.
The focus of our research has been centered for many years on the mechanisms controlling gene expression. In particular we have explored how specific chromatin remodeling, transcriptional and epigenetic systems govern a number of physiological responses. At least two areas of research are of interest: 1) The circadian clock. 2) The differentiation of germ cells. 1. The Circadian Clock. All aspects of our physiology, including the biological rhythms of feeding, hormonal production, wake-sleep cycles, motorial activity etc are regulated by the circadian clock, a molecular pacemaker based on transcriptional autoregulatory loops. We have been exploring both the physiology and genetics of this system, and unraveled some of the most surprising and fascinating links with chromatin remodeling, cell cycle and metabolism (see for example Cell (2006) 125, 497-508). 2)The differentiation of germ cells. The generation of totipotent stem cells occurs through gametogenesis, a complex, sex-specific differentiation program (for an overview of our interests see Nature (2005) 434: 583-589). Germ cells have the unique capacity to start a new life upon fertilization. The mechanisms of gene regulation in germ cells are highly specialized, and we have identified a number of unique molecular devices that germ cells utilize to elicit a fine control of pre- and post-meiotic transcription. In addition, unique epigenetic modifications exist in germ cells, including a remarkable set of germ cell-specific histone variants and a highly specialized use of DNA-methylation. Their function in chromatin remodeling, meiosis and gene expression is crucial. In the male, the haploid germ cells undergo the spectacular histone-to-protamine transition process which reshapes the nucleus and prepares it for fertilization. The epigenetic control of these processes has a number of outstanding features and its comprehension is likely to have far-reaching implications for human health and reproduction. The unraveling of the regulatory pathways and molecular mechanisms that govern the differentiation program of germ cells is likely to provide essential hints to the biology of stem cells of non-germinal origin. In particular, our studies have identified highly specialized pathways for the control of RNA processing which determine the timing of the differentiation steps leading to germ cells maturation.
REQUIREMENTS TO PARTICIPATE: High quality students with optimal scientific knowledge will be selected for their drive and enthusiasm. Interest at working at the bench and natural sense for experimentation are essential. Selection criteria: Resume and interview.
Minimum of 1 year commitment. Strong preference will be given to students with a 2 year commitment
COURSE COMPLETION:
GRADE POINT AVERAGE: 3.0 and higher
TIME COMMITMENT PER WEEK: Minimum of 12 hours/week
NUMBER OF UNITS REQUIRED: 4 units required
OFFICE LOCATION: 2115 Gillespie Neuroscience
Contact:
Paolo Sassone-Corsi: psc@uci.edu, (949) 824-4540
Leonard S. Sender, M.D.Medicine, Division of Hematology
RESEARCH DESCRIPTION: Comprehensive investigation of adolescent and young adult (AYA) cancer patients (ages 15 to 39 years) with an emphasis on hematologic malignancies, bone marrow and stem cell transplantation, fertility preservation and epidemiology. Biomedical research includes investigation of biological differences seen in AYA cancer patients as well as better defining and advancing the field of bone marrow and stem cell transplantation. The inclusive research vision incorporates research from social ecology to address psychosocial difficulties, law and public health to deal with and correct current health policy and education, epidemiology to investigate the role of environmental influences, and biomedical ethics to concentrate on long-term effects of cancer therapy in adolescents and young adults.
REQUIREMENTS TO PARTICIPATE: Students must be in their junior or senior year at UC Irvine, in good standing with the University and the School of Biological Sciences, and have, at a minimum, a 3.0 GPA. Academic interests should match one or more of the research goals of the clinical faculty member. Students should be prepared to participate 3-4 hours for each enrolled unit of Bio 199. Interested parties should have passed Bio 194S Safety and Ethics for Research and have completed the electronic research tutorials (HIPAA, human subjects) offered through UC Irvine Office of Research. Additional environmental health and safety training will be provided as needed. Students should be willing and able to work independently on assigned tasks and able to manage multiple assignments at one time while meeting defined deadlines. Successful candidates should demonstrate a willingness to learn and incorporate methods from other disciplines into their investigation of adolescent and young adult cancer.
COURSE COMPLETION: Interested parties should have passed Bio 194S Safety and Ethics for Research and have completed the electronic research tutorials (HIPAA, human subjects) offered through UC Irvine Office of Research.
GRADE POINT AVERAGE: Students must have, at a minimum, a 3.0 GPA. Exceptions may be made on a case-by-case basis.
TIME COMMITMENT PER WEEK: Students should be prepared to participate 3-4 hours for each enrolled unit of Bio 199.
NUMBER OF UNITS REQUIRED: Minimum 2 units; prefer 3 or more units.
OTHER: Work location may vary. Students must have reliable transportation.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Research presentation and/or publication at the end of rotation, participation in lab meetings, commitment, and professionalism.
OFFICE LOCATION: UC Irvine Medical Center, 101 The City Drive South, Building 56, Room 205, Orange, California, 92868
RESEARCH DESCRIPTION: Acupuncture is a major modality for disease treatment in oriental medicine. Clinical evidence suggests that acupuncture is effective for some but not all disorders, but the clinical efficacy of acupuncture remains controversial in the scientific community. Recent neuro-imaging data using functional MRI further indicate that acupuncture at certain body points (acupoints) could activate specific and non-specific areas in the brain. However, different research groups have reported inconsistent results which can be attributed to different experimental designs and technical parameters used. Possible parameters that may affect outcomes include post-treatment residual effect, stimulation pattern, duration of needle retention, sham acupuncture, fMRI image processing & analysis. Our ongoing research work involves experimental analysis for critical design factors and technical parameters that would affect the study outcomes of acupuncture. This analysis can be used to determine whether the methods of a study are sound. The ultimate goal is to establish a standardized protocol so that results from different research groups can be compared and integrated.
REQUIREMENTS TO PARTICIPATE: a reasonable knowledge of using computer software to do image analysis.
TIME COMMITMENT PER WEEK: 9 to 12 hours per week.
RESEARCH DESCRIPTION:
Synapse formation and neuronal plasticity. Much of what is known about synapse formation has come from studies of the vertebrate neuromuscular junction where a protein called agrin is responsible for the motor neuron induced differentiation of the postsynaptic apparatus in the muscle fiber membrane. Agrin’s action in muscle is mediated by a receptor tyrosine kinase called MuSK. Agrin is also expressed by neurons in the brain where it may also serve a synaptic function. However, we have shown that agrin’s target on neurons is a sodium pump rather than MuSK, suggesting that agrin’s effects in brain are due to an ability to modulate neuronal excitability by controlling sodium pump activity. Interestingly, this model of agrin action extends to other excitable cells since mutation of the agrin gene is associated with changes in cardiac muscle function. We are using cellular, molecular, biochemical, and electrophysiological approaches to further understand the mechanism of agrin action in neurons and other cells.
REQUIREMENTS TO PARTICIPATE:
COURSE COMPLETION: B+ or better in Bio 93
GRADE POINT AVERAGE: Minimum GPA of 3.3
TIME COMMITMENT PER WEEK: Minimum of 12 hours/week
NUMBER OF UNITS REQUIRED: 4
OTHER: Sophomore preferred
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Student will produce a written summary and make oral presentation of project at the end of each quarter.
One – two openings. Work with EEG measurements in response to visual signals. To assist in data analysis, the students must have computer facility and be willing to acquire minimal facility with the Matlab programming language. For students who have taken Bio. 182, there are some projects related to material covered in course.
Michael Stamos, Department of Colon and Rectal Surgery
RESEARCH DESCRIPTION:
Description: Students will be exposed to colorectal surgery, minimally-invasive surgery and surgical endoscopy. Your experience will span the spectrum of clinical research. You might begin a project and help create a study based on recent research or assist in writing an IRB (institutional review board) proposal, enrolling patients in ongoing studies, data compilation or writing/presenting your research. Your experience as a research associate will be enhanced by being assigned to faculty and staff as liaisons for each project you work on. Wednesday afternoon research meetings will help to monitor progress as well as assist in furthering your research experience. Opportunities for publication and possibly presentation at local conferences are available.
REQUIREMENTS TO PARTICIPATE:
-3.25 overall GPA
-sophomore standing
-Tuesday evening availability (not always necessary)
-Attend at least one clinic weekly
-one to two year commitment
-Four hours/unit/wk
-Minimum of 2 units
-Ability to work independently as well as in a group setting
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
-Work performance
-Initiative
-Quarterly research summary.
OFFICE LOCATION:
33 City Drive South
Orange, Ca 92868
RESEARCH DESCRIPTION: Research in my laboratory is concerned with the mechanisms that underlie memory. The central question guiding research in my laboratory is, how is it that we learn and remember information such that our past experiences influence our behavior? Specifically, we use the techniques of functional magnetic resonance imaging (fMRI), traditional experimental psychology, neuropsychological studies of amnesic patients, and studies of age-related memory changes to determine how the neural systems supporting these various types of memory operate and interact.
REQUIREMENTS TO PARTICIPATE: Must be highly motivated and interested in the cognitive neuroscience of memory and in research with human participants.
COURSE COMPLETION: Open
GRADE POINT AVERAGE: 3.3
TIME COMMITMENT PER WEEK: 6+ hours
NUMBER OF UNITS REQUIRED: 2+
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Grade will be based on effort, participation, responsibility, and quality of work. OFFICE LOCATION: Qureshey 213
Arnold Starr, M.D.Neurology, Anatomy & Neurobiology
Clinically related research using brain electrical recordings.
We see patients with impairment of memory, normal aging, and disordered sensory/motor functions. Patients are examined Wednesday afternoons and if suitable will be recruited for laboratory studies using scalp recordings (evoked potentials) to analyzed of brain activities related to their problems.
Students can select working in the Clinic or working in the research lab or both, the latter only if they have sufficient time .
The patents are only seen in the Clinic on Wednesday afternoon (1-5PM) and their charts are reviewed both before and after the encounter 3 hours). The student will be expected to spend 8 hours related to the Clinic or 8- hours related to the Lab
Requirements: Course completion: Physiology E109 & Neurobiology N110, 15 hours per week. Faculty means of evaluation for course grade: work products.
A major interest in our laboratory is to discover why the brains of some species are so much larger, more complex, and behaviorally more capable than the brains of other species. Why, for instance, are the brains of parrots so much larger than the brains of California quail, even after we correct for differences in body size? To answer such questions, we compare neural development between species (e.g. parakeets and quail), try to identify how development might have been changed to yield the observed differences between adults, and manipulate development to test our hypotheses. Students learn to observe and work with avian embryos, to cut and stain brain tissue, and to analyze neuroanatomical data. After demonstrating mastery at those techniques, students are encouraged to pursue their own research projects.
Tatsuya Suda, Ph.D.Information and Computer Science
RESEARCH DESCRIPTION:
Molecular Communication is a new and interdisciplinary research area that spans the nanotechnology, biotechnology, and communication technology. Molecular communication allows nanomachines to communicate using nano-scale molecules as communication carriers. The molecular communication research group is currently designing two specific instances of molecular communication and investigating the dynamic characteristics through computer simulation and biological lab experiments. Participating undergraduate students will be mainly responsible for computer simulation and literature survey.
REQUIREMENTS TO PARTICIPATE:
Commitment to the project for at least two quarters. Basic knowledge in two or more of the following areas preferable; biology, chemistry, physics, computer science and engineering. More specific areas of interests are the following (1) calcium signaling; (2) molecular motors; and (3) mathematical modeling and computer simulation of (1) and (2).
GRADE POINT AVERAGE:
3.3
TIME COMMITMENT PER WEEK:
10 hours per week
NUMBER OF UNITS REQUIRED:
2-5 units
OTHER:
Financial support available for selected undergraduate researchers willing to work extra hours. For more detail, contact Prof. Tatsuya Suda at suda@ics.uci.edu.
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Accomplishment of assignments, willingness to survey related materials, active participation in discussion. OFFICE LOCATION:
3243/3231 /3241 Bren Hall, Donald Bren School of Information and Computer Sciences
Laboratory is broadly interested in the forces that regulate the abundance and diversity of species in nature. The Lab studies the role of species interactions (competition, facilitation), plant-soil feedbacks, spatial heterogeneity and disturbance in determining patterns in plant communities. The lab applies this understanding to address problems of invasive species, environmental change, and habitat restoration. Students will work in lab, local field sites, and/or greenhouse on campus. Students can gain experience in a range of disciplines, including ecology, restoration, resource management, soil biology, and botany. Reguirements: prefer sophomore or junior standing and completion of introductory ecology. Grade point average of 3.0 or greater. Primarily for students who will make a multi-quarter commitment.
We study the role of nicotine receptors in cognitive function. Students will be able to learn more about cell and molecular biology in the neurosciences. They can practice techniques such as in situ hybridization, immunohistochemistry, western blotting, etc.
Requirements: 2nd or 3rd year students preferred,Completion of Bio. 93, grade point average of >3.0, >12 hours per week, 4 or 5 units.
Faculty Means of evalualtion for course grade: grades will be based on the quality of work.
Andrea Tenner, Ph.D.Molecular Biology & Biochemistry
Research area: Innate Immunity. Alzheimer's Disease. Requirements: GPAof 3.4, 12-16 hours per week, 3-4 units. At least one year commitment to the laboratory. Faculty Means of Evaluation for course grade: Weekly one paragraph reports, performance, 2 page end of the quarter written reports. Supervisor will be a postdoctoral fellow. Send informal transcript, area of interest, number of hours committed to research.
Division of Gynecologic Oncology.
RESEARCH DESCRIPTION:
Retrospective chart reviews designed to answer important clinical questions regarding the epidemiology, clinical evaluation & treatment, and/or ultimately, outcome of patients diagnosed with gynecologic malignancies (eg., cervical cancer, endometrial cancer, ovarian cancer, vulvar cancer, vaginal cancer). The research projects require the preparation of an application to the UC Irvine Institutional Review Board (IRB), a literature review, and review of medical records. All clinical material must be maintained in the highest confidentiality and only those students listed on the approved UCI IRB application may participate in a given study. In addition to the above listed responsibilities, the students will be trained in the creation of an xL file for data analysis. Students are encouraged to participate in the statistical analysis of the data, preparation of abstracts/posters/manuscripts. It is anticipated that each project shall result in the publication of a manuscript in a peer-reviewed scientific journal. Students are also encouraged to present their research at their Undergraduate Research Symposium.
REQUIREMENTS TO PARTICIPATE: Bio 194S Safety & Ethics Course Prior To Enrollment.
GRADE POINT AVERAGE: > 3.5
TIME COMMITMENT PER WEEK: At least 6 hours per week
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
1. Students will be assigned to work with a Resident in Obstetrics & Gynecology or a Fellow in Gynecologic Oncology. The Resident or Fellow will provide me with a written description of the level of the students’ participation, conduct, and efficiency in the study.
2. The student will also be required to make a 15 minute presentation of their research to me using a powerpoint file. OFFICE LOCATION: 101 The City Drive South
Building 56, Room 275
University of California, Irvine – Medical Center
Orange, CA 92868
We are researching for students who are interested in virology. We have two research projects – 1) to investigate the mechanism of persistent virus infections and their role in neurological diseases. 2) to investigate the antibody response in HIV infection and their use as a part of a vaccine. The students will be trained in molecular biology and immunology. Requirement: We require that the students are staying in the lab for at least one year or longer and spend at least 12 hours per week at the lab.
Clinical research on
1) pain and guided imagery
2) spasticity in developmentally disabled
Requirements: UCI undergaduate who will spend 1-2 years with me and my colleagues. 2 units. 4 hrs/wk. Office: UCIMC RM 17 Bldg 53.
Four Bio. 199 positions available in both summer and fall. Students would conduct projects studying how global change affects soil microbes or plants. Requirement: must have a block of time 3 hours or longer during the week in order to conduct laboratory work.
N.D. Vaziri, M.D.Division of Nephrology and Hypertension
Our research is directed at elucidation of the mechanisms and treatment of hypertension, oxidative stress, inflammation and lipid disorders in animal models of chronic kidney disease and hypertension. The studies involve cultured cells and small animals including rats and mice using biochemical and molecular biological techniques. Students in their second year and beyond who have had prior laboratory experience would be desirable.
WILL START ACCEPTING BIO 199 STUDENTS FOR WINTER 2009. RESEARCH DESCRIPTION:
The Health Education and Language for the Latino Community (HEAL-LC) is a 3-year research project in medical education funded by the Health Resources and Services Administration. HEAL-LC will develop a longitudinal Family Medicine resident curriculum in Spanish language and Latino culture that incorporates didactic sessions, practical experience in the residents’ clinic, cultural immersion experiences in the local community, home visits, and community outreach. We will develop an evidence-based, culturally oriented disease management model focused on two common problems: diabetes and hypertension. We will examine multiple outcomes from this intervention, including 360-degree evaluations involving patients and translators, objective structured clinical examinations emphasizing language and culture in patient care, objective measures of disease management performance in the resident practices, and the number of residency graduates using their training in practicing with underserved Latino patients.
Overall, we hypothesize that our language and cultural curriculum will improve patient care and increase the number of residency graduates who continue to work with underserved patients after graduation from the program.
Students will help study investigators with development of the resident curriculum and evaluation instruments. They will also help with patient recruitment.
REQUIREMENTS TO PARTICIPATE:
Competent Spanish language skills.
Students must complete requirements for human subject research and Biosafety 194 and Research Ethics course.
A strong interest in medical care for underserved patient populations.
COURSE COMPLETION: Students will be assessed on a quarterly basis.
GRADE POINT AVERAGE: Minimum of 3.0
TIME COMMITMENT PER WEEK: Minimum of 10 hours
NUMBER OF UNITS REQUIRED: 4
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Based on the diligence, creativity, and compassion of the student. OFFICE LOCATION: UCI Family Health Center
800 N. Main St.
Santa Ana, CA 92701.
Several research studies involving the role of biological and behavioral processes in human pregnancy on fetal/infant development and health (behavioral perinatology). Emphasis on the role of maternal stress and social support, maternal-placental-fetal endocrinology, immunology and genetics, fetal neurodevelopment, and biological and psychosocial markers in infancy of disease risk. Require highly motivated students with an interest in medicine, psychology, basic biology, or epidemiology and public health, who are willing to work 10-12 hours/week for 3 consecutive quarters. Training will be provided.
Our laboratory investigates the link between apoptotic/cell death signaling and activation of lymphocytes in the immune system. This link is essential for immunological homeostasis and for the prevention of autoimmune diseases. We are also broadly interested in understanding how cell death signals are propagated. We utilize a number of biochemical, genetic and molecular techniques to address these issues. Requires 1 year commitment. Completion of Bio 99; Bio 121 if possible. GPA 3.0 or better. 10 hrs/wk. 3-5 units. Faculty means of evaluation for course grade: Effort and time put into project; results; end of quarter report.
Dr. Wang's research during the last two years have provided important insights into how IGF I regulates cardiac muscle gene expression, myocardial structure, and cardiac function. Dr. Wang is a leading authority in the area of IGF-1 actions in the heart. His laboratory is now studying how heat shock proteins modulate signaling pathways of IGF-1. In addition to IGF-1 and insulin, Dr. Wang has been searching for novel hormone/factors that may be used to treat animal models of heart failure. Several ongoing projects in Dr. Wang's laboratory will continue to explore new frontiers in cardiovascular hormone actions. In addition to these projects, Dr. Wang is also a member of an international team studying the genetics of Type 2 diabetes.Dr. Wang's research during the last two years have provided important insights into how IGF I regulates cardiac muscle gene expression, myocardial structure, and cardiac function. Dr. Wang is a leading authority in the area of IGF-1 actions in the heart. His laboratory is now studying how heat shock proteins modulate signaling pathways of IGF-1. In addition to IGF-1 and insulin, Dr. Wang has been searching for novel hormone/factors that may be used to treat animal models of heart failure. Several ongoing projects in Dr. Wang's laboratory will continue to explore new frontiers in cardiovascular hormone actions. In addition to these projects, Dr. Wang is also a member of an international team studying the genetics of Type 2 diabetes.
Research involves Drosophila genetics and molecular biology and will require a minimum time commitment of 12 hours per week. Preference for motivated sophomores and juniors with a minimum 3.0 GPA in Bio. courses.
Virus Research. Herpes simplex virus.
My lab is interested in understanding the molecular mechanisms of herpes simplex virus type 1 (HSV-1) latency and pathogenesis. Following ocular infection, HSV 1 establishes life long latency in sensory neurons of the trigeminal ganglia. Sporadic spontaneous viral reactivations produce shedding of virus in tears, spread of virus to other individuals, and recurrent ocular disease. Recurrent ocular herpes infection is a major cause of viral induced blindness. Elucidation of the underlying molecular mechanisms behind the HSV 1 latency-reactivation cycle and the immunopathological and molecular mechanisms leading to corneal scarring, should lead to development of means for reducing the incidence of HSV-1 induced blindness. Projects include: 1) molecular biology of how LAT, the only one of over 80 viral genes abundantly expressed during latency, enhances the viral reactivation phenotype via its anti-apoptosis activity; 2) determining if LAT expresses a functional protein or whether it blocks apoptosis via a direct RNA activity; 3) determining if LAT enhances the reactivation phenotype by functioning at the time of latency establishment, reactivation, or a combination of both; and 4) in vivo and ex vivo confocal microscopy directed at the immunopathological, cellular, and molecular mechanism(s) involved in recurrent HSV 1 induced corneal scarring (herpetic stromal keratitis). Techniques that will be learned and used include DNA cloning, sequencing, Southern and Northern blots, RT-PCR, qRT-PCR, RNA isolation, laser microdissection, cell culture, transfection, immunofluorescent microscopy, Western blotting, construction of viral mutants, and confocal microscopy. REQUIRED BACKGROUND OF STUDENT: General biology. Microbiology, Biochemistry, Genetics, helpful. Three positions available. MINIMUM HOURS PER WEEK REQUIRED:10. One year commitment required.
Research concerns how auditory information is learned and remembered in the brain of rats. Use of behavioral, pharmacological, neurophysiological and anatomical techniques. Up to 30 hours per week during summer, 10-15 hours per week during a two year period.
We are investigating the consequences of assortative mating by flowering time in plants. The idea is simple-early bloomers will exchange pollen with other early bloomers and late will exchange with late. However, there are several potentially important consequences from this form of non-random mating, including faster evolution for plant developmental rates, higher speciation rates, elevated inbreeding levels, and potentially slower evolution of stress resistance mechanisms. Most importantly is the possibility of accelerated adaptation to some components of global change. Greenhouse and filed experiments with the plant Brassica rapa, the wild mustard, are under way. Students entering the lab spend 1-2 quarters as assistants on larger projects under the direction of myself, a post-doc or grad student. Continuing students purse their own independent project. Completion of Bio E106 required, 3.0 GPA and potential career interests in research or environmental protection.
Gregory Weiss, Ph.D.Molecular Biology and Biochemistry
The Weiss Laboratory pursues both chemical and biological aspects of chemical biology. Using chemistry to advance a molecular understanding of biology, the lab dissects key events in biology with exceptionally diverse combinatorial libraries as atomic-scale scalpels. Our libraries, collections of different molecules, include virus-displayed proteins and chemically or enzymatically synthesized small molecules. Libraries displayed on the surfaces of viruses also offer essentially universal molecular recognition for chemical sensors, illustrating how biology can advance chemistry. Undergraduates play key roles in this research, including conducting experiments both in teams and individually and co-authoring publications. Requirements: Strong interest in graduate study, competitive GPA and grades in key chemistry/biology courses, 15+ hours per week commitment, and >1.5 years remaining of study at UCI.
In vitro and in vivo studies of mechanisms of neurodegeneration and strategies for protecting neurons.Specific current areas of focus include, Roles of calcium and zinc ions in excitotoxic injury: routes of entry, intracellular regulation, and intracellular effects; Ca permeable AMPA/kainate channels:their expression, function and roles in neural signaling and in selective neurodgeneration; Mitochondrial dysfunction and free radical generation in neuronal injury; Cellular mechanisms of nerve cell loss in Alzheimer's disease, Parkinson's disease and Amyotrophic Lateral Sclerosis; Trophic factors and interneuronal signaling in neuronal survival and growth; in vitro models. Required: one year of chemistry, courses in general biology and neuroscience. Strongly recommended, Biochemistry course. Selection criteria: ability to work independently, neat and thorough work habits, GPA of 3.3 or above.
Flowering plants have a great diversity of breeding systems. Some species are hermaphroditic, others have females and hermaphrodites, and still others have only males and females. Our lab is interested in factors that promote these different breeding systems and the genetic potential of plants to evolve these different breeding systems. Understanding these processes provide information important in conservation, agriculture, and basic sciences. Undergraduates are directly involved in research and work closely with faculty and students in the greenhouse and laboratory on the rare Hawaiian genus Schiedea and on the genus Oxalis. Interest in ecology, conservation and/or evolution required; must have at least 2 years remaining at UCI; prefer GPA of 3.0 or higher.
Students who can commit to 9 months to a year of involvement with research with ADHD children and adults. Have an opportunity to learn about research protocols and implementation of clinical trials of medications and other treatments. Students will be trained in reading and implementing study protocols, interview skills, achievement testing, data collection techniques and data entry. Good experience for students interested in careers in medicine, psychology or neuropsychology.
Students who can commit to 9 months to a year of involvement with research with ADHD children and adults. Have an opportunity to learn about research protocols and implementation of clinical trials of medications and other treatments. Students will be trained in reading and implementing study protocols, interview skills, achievement testing, data collection techniques and data entry. Good experience for students interested in careers in medicine, psychology or neuropsychology.
Aileen Wiglesworth, Ph.D.Family Medicine, Program in Geriatrics
RESEARCH DESCRIPTION: Our group has multiple grants for Elder Abuse and Neglect research. We study forensic markers and risk factors for elder and dependent adult abuse and neglect. We study characteristics of people with dementia and their caregivers associated with abuse. We have developed and are in the process of validating a survey for detecting elder abuse and neglect in populations of Non-Hispanic White and Mexican American older adults, including elders with dementia. Weekly, multidisciplinary Elder Abuse Forensic Center meetings are available to students, and provide opportunities to observe and interact with a wide range of elder mistreatment experts including geriatricians, psychologists, social services, law enforcement, district attorneys and domestic violence counselors.
REQUIREMENTS TO PARTICIPATE: Students must complete the clinical packet, which may be picked up from the Biological Sciences office. It is helpful if students contact Dr. Wiglesworth well in advance of commencement of the course so that they may be added to appropriate IRB protocols. Students must have an interest in aging issues. Students must have the maturity to handle difficult situations, such as interacting appropriately with elders who have dementia or have suffered physical, psychological, sexual or financial abuse or neglect by their caregivers. Use of a car to travel between study sites is required. A multi-quarter commitment is strongly preferred. Knowledge of Spanish is a plus. Interested students should send a resume and schedule an interview with Dr. Wiglesworth.
GRADE POINT AVERAGE: 3.0. or higher
TIME COMMITMENT PER WEEK: 8-12 hours
NUMBER OF UNITS REQUIRED: 2-3 units
OTHER: WEB SITE: http://www.centeronelderabuse.org/
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Grades will be based on quality performance of research activities as directed. OFFICE LOCATION: 2 locations: UCI Medical Center in Orange and Orange County Adult Protective Services in Santa Ana
Petra Wilder-Smith, Beckman Laser Institute/Surgery
Laser use in dentistry/oral medicine for non-invasive diagnostics, therapeutics. Requirements: 2 years; pre-dental. Completed core classes for bio; intro chemistry. 3.0 GPA minimum. Min 8 hours available in 2-4 hr blocks. 2 units. Good self-motivation, good team worker, responsible, strong desire to learn to perform independent research. Faculty means of evaluation for course grade: research, publication, award performance.
Contact:
Petra Wilder-Smith: pwsmith@uci.edu, (949) 824-4713
Deborah A. Wing, M.D.Obstetrics and Gynecology
RESEARCH DESCRIPTION: We have many exciting opportunities to participate in our research program within the division of maternal fetal medicine. We have several NIH/NICHD funded research protocols, industry sponsored trial as well as investigator initiated studies. Students working on these projects will have the opportunity assist with implementation of study protocols, medical record abstraction, data collection, data entry, and the opportunity exists for laboratory processing of blood samples (i.e. centrifugation, aliqoting, to placement in long term storage). We are looking for highly motivated students with an interest in medicine, obstetrics and gynecology, maternal fetal medicine and/or pediatrics, who are willing to work 10-15 hours/week for a minimum of 1 semester. Students should have access to transportation to and from the Medical Center.
REQUIREMENTS TO PARTICIPATE: Transportation to and from the research site, minimum one semester commitment, reliable, an interested in working in medicine, Obstetrics and Gynecology, Maternal Fetal Medicine and/or Pediatrics.
Computer skills: MS Word and Excel desirable.
COURSE COMPLETION: N/A
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 10-15 hours per week
NUMBER OF UNITS REQUIRED: at least 3 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: hours completed and quality of work completed.
OFFICE LOCATION: UCI Medical Center, 101 The City Drive, Bldg. 56, Ste. 800, Orange
Mathematical and computational biology, applied to biomedical problems. We use mathematical models and computer simulations in order to understand the interactions between the immune system and viral infections. In particular, we study HIV infection, how the virus escapes immune responses, and what causes disease progression. Computational skills are required.
Brian J.F. Wong, Otolaryngology- Head & Neck Surgery, Biomedical Engineering,
Research is focused on several areas including:
1) tissue reshaping, 2) optic imaging, 3) medical device development, 4) photothermal stimulation of tissue regeneration and growth, 5) wound healing. Major interest is in cartilage tissue shape change and wound healing. Requirements: prefer Regents or Chancellor's scholars. Campus Wide Honors program. High GPA. 4 units required. Faculty means of evaluation for course grade: progress of work, independence, amount of reading.
Clinical trials and epidemiologic studies of cardiovascular disease, specifically involving 1) evaluation of prevalence and novel risk factor determinants of cardiovascular and peripheral vascular disease and their risk factors, 2) epidemiology of metabolic syndrome, and 3) epidemiology and prognosis of hypertension and its subtypes. Clinical studies currently include those involving C-reacitve protein and cardiovascular disease risk and in the future will involve surrogate markers of atherosclerosis. Requirements: Completions of all human subject tutorials and prerequisites, biology or statistical background (with introductory courses in statistics) highly recommended. Prior work or volunteer experience working with patients or customer service is helpful for those involved with clinical studies. Strong English written and verbal skills necessary. GPA 3.5 or higher desirable, but not required. 12 hours/week for a minimum of two years, including summers. 2 units or more (4 hours/unit). Interview required. Please submit resume of course, work, and volunteer experience via email indicating your status (e.g. entering junior) and number of hours available per week, to ndwong@uci.edu. Faculty means of evaluation for course grade: Initiative and performance on assigned projects.
We investigate the molecular mechanisms of memory storage using genetically modified mice. Transcriptional activation is thought to be a key process in long-lasting forms of memory. This activation is directed by transcription factors and their coactivators, which regulate gene activation via chromatin remodeling activity or histone covalent modification activity as well as mediating interactions with basal transcription machinery components. One type of histone modification associated with transcriptional activation is acetylation, which is regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs) that add or remove acetyl groups from histones, respectively. The main goal of our research is to understand how these chromatin remodeling complexes regulate gene expression required for memory processes. The research is a combined approach of molecular, biochemical, pharmacological, and behavioral analysis.
REQUIREMENTS TO PARTICIPATE: Completion of Bio N110, minimum GPA of 3.0. Ideal student will be highly motivated, work 10-15 hours/week, and make a minimum 1 year commitment.
COURSE COMPLETION: Bio 110
GRADE POINT AVERAGE: 3.0
TIME COMMITMENT PER WEEK: 10-15 hours
NUMBER OF UNITS REQUIRED: 3-4
OTHER: minimum 1 year commitment
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of research performed and understanding of topic.
Title for the course: Wu, J/Birth Study
OFFICE LOCATION: 100 Theory, suite 100
EMAIL: junwu@uci.edu
TELEPHONE: 949-824-0548
RESEARCH DESCRIPTION:
Our research will examine the impact of air pollution on adverse birth outcomes, such as preterm birth, low birth weight, and pregnancy complications including preeclampsia. We will prospectively recruit pregnant women at their first prenatal visit, follow their time-activity patterns, and develop methods to estimate their exposure levels to important air pollutants. Students will have the opportunity to learn about recruiting, consenting, and interviewing human subjects in clinical research environment.
REQUIREMENTS TO PARTICIPATE: Students must complete requirements for human subject research and Biosafety 194 and Research Ethics course and have strong social communication skills.
COURSE COMPLETION: Students must be in their junior or senior years.
GRADE POINT AVERAGE: Minimum GPA of 3.0 is required.
TIME COMMITMENT PER WEEK: 12-20 hours (4 hours per unit) per week is required but the hours can be spread throughout the week.
NUMBER OF UNITS REQUIRED: 3 - 5
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
Enthusiasm and regular completion of committed time, and quality of work. OFFICE LOCATION: 100 Theory, suite 100
RESEARCH DESCRIPTION:
The overall interest of Dr. Xiangmin Xu's laboratory is to understand organization and function of cortical circuits, with a particular emphasis on identified neuronal types and microcircuits. Currently we focus on examining local connectivity and in vivo physiology of inhibitory neurons in the cerebral cortex, by using combined approaches such as electrophysiology, laser scanning photostimulation, optical imaging and transgenic mice.
Participating students will work with graduate students and postdoctoral scholars, and help with neuroscience research. They will gain hands-on experience, and learn how to collect data, analyze data. They will also learn how to read literature and write scientific papers, and present their work at lab meetings and other occasions.
REQUIREMENTS TO PARTICIPATE: Students should be organized, motivated and detail-oriented. He/she should be able to work relatively independently, and have a good teamwork attitude.
COURSE COMPLETION: See Bio Sci 199 Standards & Policies
GRADE POINT AVERAGE: 3.0 or above
TIME COMMITMENT PER WEEK: 15 hours
FACULTY MEANS OF EVALUATION FOR COURSE GRADE:
For Bio Sci 199 the grade of A+ will be awarded only when the research is beyond excellent and includes such achievements as publications and research presentations or awards at scientific meetings.
A - Excellent (4.0 grade points awarded per unit) B - Good (3.0 grade points per unit) C - Average (2.0 grade points per unit). Plus and minus suffixes may be attached to the grades A, B, C. LOCATION OF RESEARCH: 348 Med Surge II
Good chemistry and biology background. Biochemistry preferred. Gpa of 3.3, motivation, commitment, good learning attitude, interview required. Studying mechanism of chromosome dynamics and its role in genome functions, local and global structural changes of chromosomes play critical roles in regulation of various genome functions, including mitotic chromosome organization, RNA transcription, DNA replication, DNA recombination and repair.
Conduct hearing and speech experiments in normal-hearing and cochlear-implant listeners. Programming experience will be helpful but not required. 10-15 hours per week during academic year.
Conduct biochemical, cellular and behavioral pharmacology experiments. Previous biochemistry lab will be helpful but not required. 10-15 hours per week during academic year.
RESEARCH DESCRIPTION:
Tumor suppression pathways in glioma: Gliomas are glia neoplasm in brain. One of the challenges in the treatment of glioma is the frequency of recurrence, resulting from residual highly invasive tumor cells after tumor removal. Glioblastoma multiforme (GBM) is the most common glioma. At present, there are no effective therapies for GBM, and the prognosis is extremely poor. Identifying molecules and the regulatory pathways that are responsive for the malignant nature of glioma may provide insights into how to develop new, more effective treatments for these tumors. A gene encoding a transcription factor PAX6 was suggested by our previous studies to function as a gatekeeper from further pathological processing in GBM. This project focuses on characterization of PAX6-mediated tumor suppression pathways that are responsible for the pathological hallmarks of GBM (microvascular amplification, central necrosis, and tumor invasion) and resistance to clinical therapy, and its therapeutic potential.
Characterization of genetics and evolution of brain tumor stem cells: GBM is characterized by a histologically heterogeneous cell population leading to the name “multiforme”. The heterogeneity that characterizes these cancers suggests that powerful selective pressures on genetic changes in critical target cells drive a rapid evolution of GBM onset and progression. Our study is focused on the genetic changes within the tumor stem cells and microenvironmental subpopulations and the relative contribution/importance of each in the progression of GBM.
REQUIREMENTS TO PARTICIPATE: Previous biochemistry/molecular biology lab will be helpful but not required.
GRADE POINT AVERAGE: 3.5
TIME COMMITMENT PER WEEK: 10-15 hours per week during academic year.
NUMBER OF UNITS REQUIRED: 3-4 Units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Participation in research activity, lab meeting, and participation in Excellence in Research Symposium / Undergraduate Research Opportunities Symposium / Co-author on research article from the lab. OFFICE LOCATION: MedSci C214
RESEARCH DESCRIPTION: Natural Products, Molecular Targets, and Cancer Prevention and Therapy. The Urological Oncology Lab at UC Irvine Medical Center focuses on identification and characterization of novel phytochemicals, their derivatives for prevention and treatment of prostate and bladder cancers, and the study of the mechanisms of their actions. Current projects include (1) The role of secreted Wnt inhibitors in prostate cancer tumorigenesis and progression; (2) Lycopene and docetaxel in treatment of hormonal-refractory prostate cancer: an IGF-I receptor targeting approach; (3) Novel kava chalcones, Flavokawains, in chemoprevention of prostate and bladder cancers. (4) Targeting androgen receptor signaling for treatment of hormonal-refractory prostate cancer. These research projects are currently funded by the National Cancer Institute and American Institute for Cancer Research. Students will be assigned to a small project by Dr. Xiaolin Zi, depending on the commitment and enthusiasm of the student to the project, and work in collaboration with several post-doctoral fellows in the laboratory. The students will be trained with experimental techniques, including cell culturing, DNA and RNA isolation, cDNA synthesis, PCR, immunocytochemistry, immunofluorescence, Western blotting, flow cytometry, and ELISAs.
REQUIREMENTS TO PARTICIPATE: Complete clinical package (Packet B) to work at UCIMC laboratory.
COURSE COMPLETION: Completion of Bio. 194 Safety & Ethics is required and a basic course in biochemistry/chemistry would be preferable.
GRADE POINT AVERAGE: GPA of 3.5 or better
TIME COMMITMENT PER WEEK: Would prefer a minimum of 15-20 hours/week for at least a year.
NUMBER OF UNITS REQUIRED: 3 to 4 units
FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Participation, Quality of Work, and Development of Independence. LOCATION OF RESEARCH: UC Irvine Medical Center, Bldg. 55, Rm. 360
