Bio 199 Positions Document

Bio 199 Positions

Behnoosh Afghani, M.D. Pediatrics
RESEARCH DESCRIPTION: Research in different areas related to A) Infection prevention, including hand hygiene compliance in healthcare workers and surveillance of hospital acquired infections through chart studies, B) Mentorship: Needs of students for mentorship will be assessed and the information will be used to guide planning, implementation, evaluation, and decision making to develop mentorship programs related to the field of healthcare. Projects will be conducted at the UCI Medcial Center. REQUIREMENTS TO PARTICIPATE: Students must complete requirements for human subject research and Biosafety 194 and Research Ethics course and have strong analytical and social skills. Familiarity with the Excel program will be helpful. COURSE COMPLETION: Students must be in their junior or senior years. GRADE POINT AVERAGE: Minimum GPA of 3.0 is required. TIME COMMITMENT PER WEEK: 3-4 hours per week is required but the hours can be spread throughout the week. Students can continue the project throughout the year and get units each quarter. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Commitment and enthusiasm in finishing assignments and helping with data analysis. OFFICE LOCATION: UCIMC, 101 City Drive, Bldg 58, Orange, CA 92868
Contact: Behnoosh Afghani: bafghani@uci.edu, (714) 456-5726

Anshu Agrawal, Medicine/Immunology
The focus of my lab is to understand the biology of dendritic cells (DC) of the immune system. DCs are the major antigen presenting cells that are critical mediators of both innate and adaptive immune responses. We are mainly interested in understanding the differences in the functioning of DCs between young and aged human subjects and the consequences and mechanisms underlying these differences. We study the activation of DCs by Toll like receptors (TLRs) ligands by Flow cytometry and ELISA. Signaling differences are studies by Western and flow cytometry. The priming of T cells by DCs forms a major component of our adaptive immune system studies. Office location: Med Sci I, C-238 Should work for at least 1 school yr. GPA: 3.5+. 12-15 hrs/wk.
Contact:

Thomas Ahlering, M.D. Urology
RESEARCH DESCRIPTION: Dr. Ahlering’s research focuses on the diagnosis, treatment, and clinical outcomes of prostate cancer. Dr. Ahlering is continuously trying to find new methods of faster return to continence and potency post radical prostatectomy. The Bio199 student will be involved in clinical research projects by helping to carry out studies, collecting data, maintaining study databases and analyzing data. He/She will be expected to meet with Dr. Ahlering and his research team on a weekly or biweekly basis to give updates on the clinical outcomes and findings of the data analysis. The student may be a co-author on manuscripts depending on the level and quality of work and analysis produced during his/her time as part of the research team. REQUIREMENTS TO PARTICIPATE: Major in the Biology Department At least a third year undergraduate COURSE COMPLETION: To be determined GRADE POINT AVERAGE: Minimum 3.0 GPA TIME COMMITMENT PER WEEK: Two to three days a week, about 10 hours. NUMBER OF UNITS REQUIRED: 2 - 4 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance, database upkeep, and quality of work. OFFICE LOCATION: UCI Medical Center, City Tower 333 City Blvd West, Ste 2100 Orange, CA 92868
Contact: Rosanne Santos: rtsantos@uci.edu, (714) 456-8176

Steven Allison, Ecology and Evolutionary Biology
RESEARCH DESCRIPTION: My lab works at the interface between microbiology, ecosystem ecology, and global change. We study how microbes “make a living” in the environment, and how they affect ecosystem processes, such as the break-down of dead plant material and the cycling of nutrients. Student projects may focus on field work, laboratory studies, and/or computer modeling. Summer and academic year positions are available. REQUIREMENTS: Students should be prepared to develop independent projects and spend at least 10 hours per week on research. A multi-quarter commitment is strongly preferred, and students will be expected to participate in lab activities and meetings. MEANS OF EVALUATION: grades will be based on time committed to the project and the quality of research. OFFICE LOCATION: 307 Steinhaus
Contact: Steven Allison: allisons@uci.edu, (949) 824-2341
Website: http://webfiles.nacs.uci.edu/allisons/public/index.html

Hoda Anton-Culver, Ph.D. Epidemiology
RESEARCH DESCRIPTION: Hoda Anton-Culver, PhD.focuses her research in the area of genetic epidemiology of cancer. One study funded by the NCI as an R01 combines techniques in genetic epidemiology and molecular genetics to define and characterize inherited breast/ovarian cancer in the population and in the familial component of the disease. This study began in the late 1980’s and continues to date through competing renewals of the NCI funding. Another NCI funded R01, focuses on colon and rectum cancers to study the effects of genetic influence on the mismatch repair genes as they interact with diet, physical activity, and other factors that may influence the risk for colorectal cancer and estimate genetic and environmental interaction. In addition to breast and colorectal cancer she has funding to investigate genetic and environmental influences on the risk of multiple other cancers including prostate, malignant melanoma and childhood cancer. She has been awarded several significant awards in cancer genetics from the National Cancer Institute. These awards include the (1) "UCI-UCSD Cancer Genetics Network" to collaborate with other centers to investigate the genetic basis of human cancer susceptibility and identify means to address the public health issues associated with human cancer genetics; (2) The "NCI Cancer Genetics Network Informatics Center at UCI" supports the cooperative of Cancer Genetics Networks as the sole Informatics Center responsible for the design, implementation, and maintenance of an information management system that supports the Network-wide research protocols REQUIREMENTS TO PARTICIPATE: Biology, Genetics, Statistics, and Epidemiology COURSE COMPLETION: at least 2 courses of the above are required GRADE POINT AVERAGE: GPA 3.0 minimum TIME COMMITMENT PER WEEK: 8- 12 hours NUMBER OF UNITS REQUIRED: 2-3. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Practical, oral and written evaluation. OFFICE LOCATION: 224 Irvine Hall, Zot 7550
Contact: Hoda Anton-Culver: hantoncu@uci.edu, (949) 824-7416

Tallie Z. Baram, M.D., Ph.D. Anatomy/Neurobiology;Pediatrics
Neuroscience Research. Cellular mechanisms of vulnerability of the developing brain to febrile and other seizures. Mechanisms of excitation-dependent neuroplasticity. Molecular neurobiological mechanisms of response to stress during development.
Contact: Tallie Baram: tallie@uci.edu, (949) 824-1131, (949) 824-1063
Website: http://www.ucihs.uci.edu/anatomy/baram.html

Stanley Bassin, Ed.D. Cardiology
Lab or building where research will be conducted: Field Work in Orange County and Long Beach with Community Health Organizations. CURRENT RESEARCH INTERESTS AND DESCRIPTION: Conducting Research to prevent chronic disease (cardiovascular, diabetes, obesity and build bone health) through lifestyle interventions in minority communities through school and community programs. Research Studies currently involve preventing type 2 diabetes with middle school children and their families as well as the establishment of network for communicating research findings to the diverse population of Orange County PREREQUISITES: Upper division students who have completed Bio 194 and ethics course Completion of all human subject tutorials and prerequisites, biology or statistical background (with introductory courses in statistics) highly recommended. Prior work or volunteer experience working with diverse communities. Strong English written and verbal skills necessary. Grade Point average of 3.0 or higher SELECTION CRITERA: Resume, experience in and /or interest in community health, writing experience, and social skills to work in diverse communities. Upper Division standing. Interview will be required. Please submit resume of course work, volunteer experience and number of hours available per week via e-mail to slbassin@uci.edu UNIT REQUIREMENT: Number of Units/Quarter 2 or more units/per quarter (4 hours per unit) Hours/Week 8 hrs per week GRADING BASIS: Examination: Initiative and research paper to be submitted and /or develop a proposal for funding with a community organization. UCI faculty member will evaluate quantity of Work Research paper and / or funding proposal for content and exposition
Contact: Stanley Bassin: slbassin@uci.edu, (949) 824-8856

Anjan Batra, M.D. Pediatrics
RESEARCH DESCRIPTION: There is a high incidence of arrhythmias in patients undergoing the Fontan surgery for palliation of single ventricle hearts. The incidence of these arrhythmias using two different surgical techniques is unclear. The purpose of this research is to compare the incidence of arrhythmias between these 2 different surgical techniques (lateral tunnel vs. extracardiac conduit). REQUIREMENTS TO PARTICIPATE: Understanding of human cardiac anatomy. TIME COMMITMENT PER WEEK: 10 hours NUMBER OF UNITS REQUIRED: 3 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Completion of an abstract. OFFICE LOCATION: 101 The City Dr.
Contact: Anjan Batra: abatra@uci.edu, (714) 456-2331

James Belluzzi, Ph.D. Pharmacology
The projects examine the effects of nicotine in adolescent and adult rats. Will learn to perform survival surgery on rats, and intend to work in the lab for 1 – 2 years. The work requires daily animal handling, sterile preparation of drugs, and careful performance of the experiments. Accepts students that have GPA greater than 3.0, can work in the lab 12 – 16 hours per week spread over three days.
Contact:

Lbachir BenMohamed, Ph.D. Ophthalmology and Center for Immunology
Lbachir BenMohamed, Ph.D. and Anthony B. Nesburn, M.D. OFFICE LOCATION: UCIMC, Building 55, 2nd Floor, Room 202 RESEARCH DESCRIPTION: Immunology of Herpes Simplex Virus (HSV) Type 1 Latency and Pathogenesis The aim of this study is listed in three sections: 1) To develop a clinically acceptable vaccine strategy to reduce or eliminate ocular and genital herpes infection including recurrent disease. 2) Eventually, the candidate vaccines developed in mice will be tested in rabbits and humans. Techniques used in this project: Immunology, virology, and molecular biology - techniques that include vaccine studies in animal models. 3) To investigate if (1) LAT is involved in dendritic cells (DC) modulation (2) HSV-1 interferes with the migratory activity of DC in vivo (trafficking or homing), (3) LAT also plays a role in this viral activity. Development of immunotherapeutic and immunoprophylactic strategies against various cancers 1) The aim of this study is to develop a clinically acceptable Glyco lipopeptide vaccines to reduce or eliminate tumor cells 2) The candidate vaccines developed will be tested in mouse model of cancers. Techniques used in this project: Immunology, virology, and molecular biology - techniques that include vaccine studies in animal models. REQUIREMENTS TO PARTICIPATE: Requirements - General background in biology/biochemistry/immunology/virology, oncology and/or infectious diseases. Strong motivation, good organizational skills, quick study and receptive to taking work direction. COURSE COMPLETION: Completion of core through Bio 99 with a grade of B or higher, completion of Bio 194S Safety, consent of instructor. GRADE POINT AVERAGE: GPA 3.0 or higher TIME COMMITMENT PER WEEK: Minimum 25 hours per week. NUMBER OF UNITS REQUIRED: 4 OTHER: Accepts students that can work in the lab 25 hours per week spread over three days. Interested in students who will make at least a multi-quarter commitment (one to two years preferred). Currently six (6) positions available and routinely students needed on an on-going basis. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of Work and Weekly Meeting.
Contact: Lbachir BenMohamed: lbenmoha@uci.edu, 714-456-7371

Rimal Bera, M.D. Psychiatry and Human Behavior
This research is takes place at UCIMC under the direct supervision of Dr. Bera. Students will join on-going clinical trials of both inpatients and outpatients who are enrolled in clinical research programs. These trials include medication evaluations, neuropsychological testing and retrospective medical record reviews. Students will be trained in psychiatric evaluation of multiple disease states and have the opportunity to work directly with 3rd and 4th year medical students and psychiatry residents with patients who are hospitalized. Students will get state of the art clinical contact with patients that will help them in future clinical opportunities. Independent research ideas to be studied will be encouraged. Presentation at scientific meetings along with submission of findings to scientific journals will be encouraged.REQUIREMENTS TO PARTICIPATE: Motivation and interest in direct patient contact in both clinical and research setting. COURSE COMPLETION: Minimum of 6 month committment GRADE POINT AVERAGE: Minimum GPA of 3.00 TIME COMMITMENT PER WEEK: 6 to 10 hours/week NUMBER OF UNITS REQUIRED: 3 to 4 units OTHER: FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm of effort and completion of assigned work.
Contact: Rimal Bera: rbera@uci.edu, (714) 456-6898

Hans-Ulrich Bernard, Ph.D. Molecular Biology & Biochemistry
RESEARCH DESCRIPTION: Induction of cervical carcinoma and other types of cancers by papillomaviruses. Specifically regulation of papillomavirus transcription during the normal life cycle and during carcinogenesis. REQUIREMENTS TO PARTICIPATE: Preferentially 3rd/4th year student, knowledge about transcription, virology, cell biology. COURSE COMPLETION: n/a GRADE POINT AVERAGE: n/a TIME COMMITMENT PER WEEK: minimum of 8 hours (1 full day or 2 half days) plus participation in group seminar (normally Wednesday at noon), preferentially for 2 or 3 quarters. NUMBER OF UNITS REQUIRED: n/a FACULTY MEANS OF EVALUATION FOR COURSE GRADE: enthusiasm and regular completion of committed time. OFFICE LOCATION: 114 Sprague Hall
Contact: Hans-Ulrich Bernard: hbernard@uci.edu, (949) 824-5162

Michael W. Berns, Ph.D. Developmental & Cell Biology
Cell Biology/Laser
Contact:

Devin Binder, M.D., Ph.D. Neurological Surgery
COURSE TITLE: Role of Astrocytes in Epilepsy. OFFICE LOCATION: Med Sci 1, Room C356 RESEARCH DESCRIPTION: Astrocytes, the most abundant glial cell type in the brain, are involved in many important physiologic functions such as neurotransmitter, potassium, and energy metabolism. Accumulating evidence over the past few years suggests that astrocytes may also powerfully modulate brain excitability. Therefore, neuroscientists have begun to investigate the hypothesis that glial cells may contribute to the hyperexcitability of epilepsy. Indeed, pathologic specimens from patients with temporal lobe epilepsy often demonstrate marked reactive gliosis (glial cell scarring), and alterations in distinct astrocyte membrane channels, receptors and transporters have all been associated with various forms of epilepsy. Our recent research has focused on the study of the astrocyte water channel aquaporin-4. Distinct aquaporin “water channels” have been shown to be important for water transport in many different tissues. In the brain, aquaporin-4 is involved in water and ion metabolism. Recent studies have shown powerful alterations in excitability, seizure duration, extracellular space and potassium regulation in aquaporin-4-deficient mice. These results coincide with results demonstrating altered levels of aquaporin-4 in human temporal lobe epilepsy. However, it remains to be determined how the cellular expression and function of aquaporin-4 is altered during the development of epilepsy. We will be examining these and related questions in well-established animal models of epilepsy. The goal of this research is to further understand astrocyte control of water and potassium metabolism in the brain, and how it goes awry in the development of epilepsy. This could directly lead to new concepts and targets for anticonvulsant drug development that may have many fewer side effects than current therapies. Students will be directly involved in learning and contributing to the laboratory through the following techniques: Small animal anesthesia and stereotactic neurosurgery In vivo electrophysiology and pharmacology EEG recording and analysis Fluorescence imaging Histology Immunohistochemistry Confocal microscopy In situ hybridization Western blotting PCR REQUIREMENTS TO PARTICIPATE: Must be highly motivated and interested in neurosciences, particularly epilepsy and in vivo imaging. Successful performance may lead to a career in clinical/translational neuroscience, neurology, and/or neurological surgery. Prior laboratory experience desired. Introductory biology course work required. Prior neurobiology course work desired. TIME COMMITMENT PER WEEK: 10-15 hours, flexible work schedule including some weekends. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm and commitment for development of laboratory skills, research methods, and scientific acumen. Students will be involved not only in generating laboratory data, but also in literature reviews and laboratory presentations. All of these will contribute towards both their education and evaluations.
Contact: Devin Binder: dbinder@uci.edu, (949)824-0432

Bruce Blumberg, Ph.D. Developmental & Cell Biology
Our research focuses on the role of nuclear hormone receptors in embryonic development, adult physiology and human diseases such as obesity, cancer, inflammation and aging. Nuclear hormone receptors are ligand modulated transcription factors that directly regulate the expression of target genes. Students will gain experience in a broad range of research techniques including molecular biology, biochemistry, developmental biology, cell biology, cancer biology, molecular endocrinology, and genomics. Students beginning in summer and continuing through the year will be given preference, however, starting at any time in the academic year is possible for outstanding students if space is available. Students can visit the laboratory web site for more information. Generally speaking, completion of the first 2 years of the Bio Sci core is required. Exceptions have occasionally been made in the past for highly qualified and motivated students. Bio 103 and/or 104 are often helpful but not required. A GPA of 3.0 or higher is required. The nature of our research requires a time commitment of at least 20 hours per week for a minimum of one year. The possibility of a second year is advantageous as this will allow the student to undertake an independent research project. Although it is not required, students in the lab are strongly encouraged to participate in the Excellence in Research Program, the University Research Opportunity Program (UROP) and the Summer Undergraduate Research Program (SURP). Previous students have published in major scientific journals and in the UCI Undergraduate Research Journal. We may be taking students in 2007-2008. Please contact Dr. Blumberg directly if you are interested.
Contact:
Website: http://blumberg-lab.bio.uci.edu/

Stephen Bondy, Ph.D. School of Medicine
The overall project goals are to understand how aging changes gene expression in the brain and how these changes may help explain the decline in neuroimmunological function associated with aging. We use lipopolysaccharide (LPS, a toxic, inflammatory stimulus of innate immunity) dosing, and exposure to a novel environment (behavior), as modulators the assess brain function in old mice compared to young. The project will determine the degree to which old mice express these genes differently than young, and how the expression pattern of the response to LPS differs in old mice. Expression changes of one gene, known to occur when animals are exposed to a novel environment, may also differ in the old mice that have also been challenged with LPS. The occurrence of another gene, previously undetected in brain and of unknown function there, will be confirmed, and its response to age, inflammatory challenge, and novel environment will be determined. The 199 Research will consist of extraction and purification of total RNA from previously-frozen mouse brains. Then expression levels of individual gene transcripts in the RNA samples will be measured by real time (quantitative) RT-PCR. A final aspect of the project is to summarize and prepare the results for poster presentation or publication.
Contact: Edward Sharman: esharman@uci.edu, (949) 824-4769

Daniela Bota, Neurology, Cancer Center
RESEARCH DESCRIPTION: 1. Translational Research: There is an unmet clinical need for the therapy of malignant gliomas with a median survival of <12 months despite available treatments. Traditional chemotherapy relays on DNA damage and disruption of mitotic machinery, with limited effect in prolonging patient survival. The focus of my research is to test novel agents, in an attempt to target downstream molecular mechanisms involved in tumor growth and resistance to apoptosis. The final goal is to provide scientific bases for clinical trials in patients with newly-diagnoses and recurrent malignant gliomas. 2. Clinical and Quality of Life Research: The main purpose of this study is to conduct a retrospective and prospective chart review to examine associations between care plan timelines and demographic variables in a large clinic sample of patients with brain tumors. Our research aims are to (1) compute correlations among the different variables and determine the average delay of care for Spanish speaking only patients versus native English speaking patients and (2) to test if a patient navigator can improve the access to care for brain tumor patients resulting in a decrease in time from diagnosis to treatment. REQUIREMENTS TO PARTICIPATE: Motivation and interest in conducting research in both clinical and laboratory setting. Prior laboratory experience desired for the translational research. COURSE COMPLETION: Minimum of 6 month commitment GRADE POINT AVERAGE: 3.4 TIME COMMITMENT PER WEEK: 15-20 hours/week NUMBER OF UNITS REQUIRED: 3 to 4 units OTHER: Would prefer at least 15-20 hours/week over 3 days every week. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Research presentation at the end of rotation, participation in lab meetings, commitment and professionalism. OFFICE LOCATION: UC Irvine Medical Center, Bldg 55, Room 121
Contact: Daniela Bota: dbota@uci.edu, (714) 456-7032

Adriana Briscoe, Ph.D. Ecology and Evolutionary Biology
RESEARCH DESCRIPTION: Research positions are available to work on a number of projects related to studying the evolution and functional characterization of opsin genes in butterflies. Students will have the opportunity to learn molecular biological techniques such as PCR, cloning and sequencing, and after such techniques are mastered, will be encouraged to develop an independent project in their second year. Minimum requirements are at least a 1 year commitment, 3 units (9 hours/week) per quarter, mandatory lab meeting attendance and a G.P.A. of 3.3+. GRADE POINT AVERAGE: 3.3+ TIME COMMITMENT PER WEEK: 9-12 hours/week NUMBER OF UNITS REQUIRED: 3 OTHER: Mandatory lab attendance, written summary of research progress at the end of each quarter. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Successful acquisition of experimental data and lab meeting attendance. OFFICE LOCATION: MH5248
Contact: Adriana Briscoe: abriscoe@uci.edu, (949) 824-1118

Peter Bryant, Developmental & Cell Biology
Project 1). Asymmetric Division of Neural Stem Cells. Study the mechanisms of cell division in mammalian neural stem cells, using a rat model of Parkinson’s Disease or stroke. Test the hypothesis that these cells undergo asymmetric cell division, by immunostaining and confocal microscopy using antibodies against the human homologs of asymmetric cell division determinants identified genetically in Drosophila. REQUIREMENTS TO PARTICIPATE: • Interest in stem cell biology • 2-page proposal during the first two weeks • 5-page report due at the end of Finals Week. • 4 units of work (12 hours/wk). 3.5 GPA. Project 2). Monitoring Local Biodiversity. Work with the faculty advisor and other local experts to document and monitor local wildlife populations, choosing from one of the following: • Photo-documentation of local species of invertebrates (mainly insects and spiders), field studies of their distribution, seasonality and abundance, and contribution to a web site showing the life history, interesting features, and conservation issues concerning each species. • Bird counts at Upper Newport Bay and/or the San Joaquin Wildlife Sanctuary • Marine Life Inventory at Upper Newport Bay REQUIREMENTS TO PARTICIPATE: • Some knowledge of or at least interest in local natural history. • Willing to do unsupervised field work after training is completed. • Field work may require the student to obtain or rent equipment (binoculars, spotting scope, camera) and obtain a field guide. • 2-page proposal during the first two weeks • 5-page report due at the end of Finals Week. • 4 units of work (12 hours/wk). 3.5 GPA. Project 3). Campus Sustainability. Investigate the operation of the UCI campus to identify activities that can be made more independent and sustainable, and initiate student action to make improvements. Projects include use and reuse of energy and water, use of drought-resistant native plants, minimizing waste, use of biodegradable materials in food facilities, general recycling and waste reduction projects, and pollution-free transport. Methods include library and internet research, interviews with campus decision-makers, and comparison with other campuses followed by practical participation and promotional activities for improvements through student organizations. Projects will be supervised by the faculty advisor and the UCI Recycling Coordinator. REQUIREMENTS TO PARTICIPATE: • Willingness to carry out research as well as practical work at the campus recycling center. • 2-page proposal during the first two weeks • 5-page report due at the end of Finals Week. • 4 units of work (12 hours/wk). 3.5 GPA.
Contact: Peter Bryant: pjbryant@uci.edu, (949) 824-4714
Website: http://mamba.bio.uci.edu/~pjbryant/dbc/bryantp.htm http://mamba.bio.uci.edu/~pjbryant/biodiv/index/htm

William Bunney, M.D. Psychiatry and Human Behavior
TWO PROJECTS: Project #1 -- Contact Julie Patterson at (714) 957-5535 -- UCI Medical Center - This research is using brain function (electroencephalogram or EEG), neuropsychological (tests of memory, attention, etc.), symptom, and clinical interview measures to study the contribution of brain inhibitory mechanisms and information processing deficits to the symptoms of schizophrenia and bipolar disorder (manic depression). The students will have the opportunity to have direct patient contact by helping with patient testing. The testing and analysis is done at the UCI Medical Center. Students are needed on an ongoing basis.--------------------------------------------- Project #2 -- Contact David Walsh, PsyD, ABPP, at (949) 824-5013, RESEARCH DESCRIPTION: UCI Main Campus - This project is funded by the National Institute of Mental Health to examine genetic, architectural, and biochemical brain abnormalities in major depression, bipolar affective disorder and schizophrenia. This opportunity would be appropriate for students interested in research of severe mental illnesses. Students would develop a clear understanding of DSM-IV diagnostic criteria, risk factors for suicide and general research methodology. Students are needed on an ongoing basis. REQUIREMENTS TO PARTICIPATE: Motivation and interest in clinical research. GRADE POINT AVERAGE: Minimum GPA of 3.00 TIME COMMITMENT PER WEEK: 6 to 10 hours per week FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Enthusiasm of effort and completion of assigned work. OFFICE LOCATION: Main Campus – 5251 California Ave, Suite 160, Irvine, CA
Contact:

Jorge Busciglio, Ph.D. Neurobiology
This course is directed to provide students with an overall view of laboratory techniques to evaluate neuronal survival and degeneration, and to review the role of neuronal loss in neurological diseases.
Contact:

Vincent Caiozzo, Ph.D. Physiology & Orthopedic Surgery
Sophomore standing or higher. Studying hibernating muscle physiology & disuse atrophy in several non-model species, including ground-squirrels, bears, bats and marmots. Examining lizard species as well. Utilization of several molecular biology techniques. Involves analyzing the protein and mRNA profiles of myosin in muscle, using RT-PCR and SDS-PAGE. Cloning myosin genes in the above species. Summer work may involve some travel to collect samples from collaborating institutions ( Colorado and the Sierra Nevada , CA).
Contact:

Anne L. Calof, Ph.D. Anatomy & Neurobiology, Developmental & Cell Biology
199 students are needed to participate in ongoing research on neural stem cells and molecular signals that regulate development and regeneration of the nervous system. Successful 199 students from the laboratory frequently become authors on papers published in scientific journals, and may have the opportunity to present their research at national conferences. Requirements : Minimum Sophomore (for exceptional cases) or Junior standing, 3.4 GPA (this is sometimes negotiable), Minimum commitment 1 year (4 quarters), 2 years preferred, willingness to perform 1 st quarter Pass/no pass basis to learn lab techniques, must spend 8-12 hours per week, attend weekly lab meetings and present research in lab meetings.
Contact: Anne Calof: alcalof@uci.edu, (949) 824-5745
Website: http://www.ucihs.uci.edu/anatomy/calofres.html

Arezoo Campbell, Ph.D. Community & Environmental Med
The research focus of the laboratory is on delineating the mechanisms by which environmental toxins may accelerate the pathogenesis of neurodegenerative disorders. Presently we study the potential role of aluminum or copper in drinking water and particulate matter present in air pollution. Emphasis is on the role of neuroinflammatory and oxidative events. Flexible number of units required. 10 hours/wk. 3rd year student standings. 2 years of chemistry and biology courses. GPA 3.4 or greater.
Contact:

Diane R. Campbell, Ph.D. Ecology & Evo Biology
Research in my lab concern the mechanisms of evolution in natural plant populations. Current projects focus on plant hybrid zones. We are comparing the fitness of plant hybrids to that of the parental species to test particular models for genetic differentiation and evolution of new species. Approaches include experimental field studies in the Colorado Rockies and the eastern Sierras. These involve measurement of insect and bird pollinator behavior, pollination success, photosynthetic and other physiological traits, and seed production in common gardens. At UCI we do greenhouse studies of floral and physiological traits, and DNA analyses to examine the genetic structure of natural hybrid zones. Students with strong interest in ecology, genetics, and/or evolution. Bio 93 and 94, or Bio 96 required. Bio 97 and E106 are helpful. Minimum 3.2 GPA. 6-12 hours/ wk. 2-4 units required.
Contact: Diane Campbell: drcampbe@uci.edu, (949) 824-2242

F. Lynn Carpenter, Ph.D. Ecology
Sophomore standing and above. Completion of Bio. 94. I am investigating methods to reforest eroded, nutrient-deficient, toxic tropical soils where rainforest was cut for pasture 50 years ago, and the land severely overgrazed. We have many long term experiments in Costa Rica on regeneration of forest and of topsoils. Students may participate in my 199 program either by analyzing one of these ongoing experiments or by establishing their own experiment in the field in Costa Rica . Examples of potential topics include the role of soil biology and chemistry in tree growth, the characteristics of trees capable of growing in eroded soil, and the repopulation of reforested areas by birds and insects. Two quarters of preparatory research are required if a student wishes to work with us in Costa Rica in the summer. Details of our various research approaches are on the website
Contact:
Website: http://darwin.bio.uci.edu/~flcarpen/

Philip M. Carpenter, M.D. Pathology
Our interest is how normal tissues contribute to the metastasis (spread through out the body) of breast and other cancers. We have identified a basement membrane protein, laminin 5 that is secreted by normal breast cells, yet causes increased migration and spread of breast cancer cells. We are developing inhibitors to this process that might aid in patient treatment. 1 year biology, biology lab and general chemistry, at least 1 quarter of organic chemistry. Recommendation from your lab instructor is required. 12 hours minimum, 15-18 preferred. 3 units will be given for 12 hours work. GPA: B or better. The lab is at UCI Medical Center, so students should be prepared to commute and comply with additional UCIMC requirements.
Contact: Philip Carpenter: pmcarpen@uci.edu, (714) 456-6141

Paolo Casali, M.D. Center for Immunology
Research in Dr. Casali's lab is at the cutting edge of modern molecular and cellular biology. It investigates the mechanisms of class switch DNA recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin genes using in vivo and in vitro models. Students will be trained in advanced molecular biological techniques including but not limited to: aseptic technique, cell culture protocols, DNA and RNA isolation, cDNA synthesis, molecular cloning, PCR, PAGE, Western and Northern blotting, EMSA, and ELISA. Work involves human lymphocytes and/or transgenic mice. Interested students should submit an updated resume with relevant research experience. Project assignments will be determined by the commitment and enthusiasm of the student to lab work. Interested students are encouraged to visit the lab prior to requesting a position. Minimum GPA required: 3.0. In order to be considered for a bio199 position, please send a completed application to epone@uci.edu. Application url: casalilab.bio.uci.edu/application.doc Lab website: casalilab.bio.uci.edu Lab Blog: casalilab.blogspot.com
Contact:

Thomas Cesario, School of Medicine
The use of foreign antibodies for the treatment of several diseases in humans The use of specific antibodies to treat disease is of growing importance, especially for the treatment of cancer, neurological diseases, and infectious diseases. Unfortunately, the source of these antibodies is very limited since the patient has to be treated with the same antibody several times. Antibodies produced in animals are limited to a single use, as subsequent administration of the same antibodies will induce a harmful immune respone (e.g. allergic reaction) and can lead even to death. Additonal antibodies derived from animals is viewed as foreign by the human body and can elicit an antibody reaction on its own and be eliminated before having an effect. Methods for rendering these proteins safe for multiple injections and keep the antibodies functionell are being actively researched including production of hybrid monoclonal antibodies and the "humanized" animals. However, many of these methods are time consuming , expensive and complicated, and mostly specific for only one disease. We developed a new technology to make these proteins from animals safe for multiple injection, and this methode is less time consuming, less expensive and not very complicated, and it is not disease specific. Currently we want to evaluate our new technique to determine if we are able to use these antibodies for treating diseases such as infectious diseases and autoimmune diseases. Therefore, we are looking for students who are interested in immunology and infectious diseases. The students will be involved in immunological techniques such as ELISA and Westernblot and molecular biological techniques such as cloning, polymerase chain reaction , and tissue culture and also in handling the animals. Recent studies from a private Laboratory (PlasVacc USA, Templeton, CA), however, suggested that mice will tolerate multiple injections of a modified technique to produce cryoprecipitate-depleted plasma (Cryoprecipitate is a precipitate that forms when normal blood plasma is cooled, and is especially rich on clotting factors-the remaining plasma contains the antibodies). The plasma obtained with this improved technique is now termed cryoprecipitate-FREE plasma. THE DIFFERENCE BETWEEN CRYOPRECIPITATE-FREE PLASMA OR DEPLETED PLASMA IS THE AMOUNT OF THE CRYOPRECIPITATE ASSOCIATE PROTEINS. CRYOPRECIPITATE CONTAINS TYPICALLY 40% OF THE PROTEINS PRESENT IN THE PLASMA; THE REMAINING 60% ARE STILL IN THE PLASMA WHICH IS TERMED CRYOPRECIPITATE-DEPLETED PLASMA. THE CRYOPRECIPITATE-FREE PLASMA IS FREE OF CRYOPRECIPITATE ASSOCIATE PROTEINS. The mice survived three immunization with the cryoprecipitate-FREE plasma after the plasma was sterilized over a column (personal communication-see attachment). However, this company never tested, if the antibodies are still functional. Therefore, we wish to perform a study to test the hypothesis that cryoprecipitate-free plasma is 1) tolerated by animals from a different species by repeated injections and 2) the antibodies in this plasma are still functional. We will use rabbits as the plasma donors, and mice as the test subjects.
Contact: Martina Berger: mberger@uci.edu, (714) 456-5161

Jeff Chan, Ph.D. Pathology, Biological Chemistry, Toxicology
Research areas: Cell growth and function require the coordinate regulation of gene expression by transcription factos. My lab seeks to understand the role of CNC-bZIP transcription factors in oxidative stress response. Diverse molecular and genetic approaches including trasngenic and knockout mouse models, DNA-microarrays, cell culture, and yeast are being applied to delineate the physiological roles of CNC factors in cell function; their role in oxidative stress responses and the genesis of cancer; and the mechanisms by which these factors act. Biology & chemistry courses. 3 units. At least 12 hours of work. GPA 3.0 or better.
Contact:

Frances Chance, Ph.D. Neurobiology & Behavior
The brain is constantly bombarded with sensory input from the environment and must receive, process and respond to this information. The research in this laboratory seeks to understand how neurons in the cortex process information. We study this question by recording from individual living neurons in slices of brain tissue, and also by building computational models of neurons and networks of neurons. For a description of current projects, please see the listed website. Http://chancelab.bio.uci.edu For projects involving electrophysiology, the student will stimulate and record from neurons in slices of rodent brain tissue. For projects involving computational modeling, the student will build and study computational neural networks (using C, Matlab or another language mutually agreed upon by instructor and student). Prerequisites : Consent of the instructor. Ideally, student will have completed Bio 110. Student must be willing to commit for at least two consecutive terms, preferably longer. 4-5 units/quarter. GPA 3.2. Knowledge of a programming language would be extremely beneficial.
Contact:
Website: http://chancelab.bio.uci.edu

George Chandy, Ph.D. Physiology & Biophysics
Our lab studies the relationship between specialized membrane proteins, potassium channels, and disease. One of the channels we are interested in, Kv1.3, is a voltage gated channel which is upregulated in fully differentiated autoreactive T-cells. We are currently developing channel blockers for Kv1.3 as therapeutics for autoimmune disease (multiple sclerosis, type I diabetes, and rheumatoid arthritis). The second potassium channel that we study, the SK channel, is implicated in several neurological disorders and also hypertension. We have generated a transgenic mouse in which this channel's function has been silenced in dopaminergic neurons. We believe that this mouse has the potential to act as a model for ADHD (attention deficit hyperactivity disorder), drug addiction, and also hypertension. We are looking for responsible students with a GPA of at least 3.0 to carry out work in one of these areas. All of our available projects have direct clinical implications, and students will gain experience in understanding how a single class of molecules can lead to disease. We have had previous 199 students that have gone on to medical schools and top graduate programs in the biological sciences.
Contact:

Bernard Choi, Ph.D. Biomedical Engineering and Surgery, (Beckman Laser Institute)
RESEARCH DESCRIPTION: The mission of the Choi Microvascular Therapeutics & Imaging (MTI) Lab is to develop instrumentation and employ methods to improve in vivo characterization of the microvasculature. 199 students in my laboratory work in a multidisciplinary environment at the intersection of engineering, vascular biology, and clinical medicine. I strongly encourage independent research projects which encompass at least one (oftentimes several) of the following topics: 1. Optical imaging instrument development/characterization 2. Animal surgery 3. Clinical research 4. Light-based therapy monitoring 5. Computational modeling The most updated list of research opportunities can be viewed at the MTI lab website: http://choi.bli.uci.edu/ REQUIREMENTS TO PARTICIPATE: Requirements vary by project. I am most interested in students who have at least two years to commit to research in my lab. COURSE COMPLETION: Course requirements vary by project. GRADE POINT AVERAGE: >3.5 GPA is desired. TIME COMMITMENT PER WEEK: >8 hrs/week NUMBER OF UNITS REQUIRED: >2 OTHER: N/A FACULTY MEANS OF EVALUATION FOR COURSE GRADE: I evaluate productivity and effort, and ability to integrate into the existing MTI lab framework. OFFICE LOCATION: Beckman Laser Institute
Contact: Bernard Choi: choib@uci.edu, (949) 824-9491

Olivier Civelli, Ph.D. Pharmacology
RESEARCH DESCRIPTION: Areas of research: molecular pharmacology, neurobiology, drug discovery. Central theme: find novel genes involved in brain disorders. Disorders currently studied: obesity, drug addiction, memory deficits, sleep disorders, stress. Students will have opportunity to participate in experiments covering all aspects of pharmacological research: molecular (cloning) and cellular (cell culture) biology, pharmacology (binding and second messenger assays) and animal behavior (if desired). REQUIREMENTS TO PARTICIPATE: students interested in pharmacological/pharmaceutical research; commitment: 2 years or more. Preference to those with experience in biological and/or chemical techniques. GRADE POINT AVERAGE: 3.3+ TIME COMMITMENT PER WEEK: 12-16 hours/week NUMBER OF UNITS REQUIRED: 3 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: participation, willingness to learn, responsibility OFFICE LOCATION: 369 Med Surge II, School of Medicine
Contact:
Website: http://ucihs.uci.edu/pharmaco

Ralph V. Clayman, M.D. Urology
Endourology lab. Multiple large animal surgical experiments as well as other “bench top” experiments in progress on an ongoing basis. Usually the student is assigned a project as primary research and also participates in all of the other experiments as well. Part of the job involves setting up and breaking down the lab for animal experiments and working with the animals (pigs, rabbits) directly after appropriate training. The level of responsibility is graded depending on the experience level of the student and increases as time goes on. A.) Tissue bioengineering: bladder replacement b.) Prosthetics and other surgical materials: bladder replacement, stent development c.) New technology for laparoscopic surgery d.) Studies on endoscopes e.) Clinical research into laparoscopic surgery outcomes in Urology Science back ground and interest in surgical research. No prerequisites. 4-10 hours/wk. GPA 3.25 or above.
Contact: Ralph Clayman: rclayman@uci.edu

Susana Cohen-Cory, Ph.D. Neurobiology
Laboratory based research investigating mechanisms of neuronal development, with particular emphasis in the use of embryological and microscopy imaging techniques. Requirements: Bio. Majors and completion of freshman year that includes 1 yr. of general chem. With labs and Bio. 94 and 96.
Contact:

Dan Cooper, M.D. Pediatrics
Prevalence of Type II diabetes in middle school children. Research involves LA & Orange counties.
Contact: Frank Zaldivar: fpzaldiv@uci.edu, (714) 456-6914

Carl Cotman, Ph.D. Neurobiology & Behavior
Assist in neurobiological studies comparing normal and abnormal brain aging (both animal and human). The primary focus will be using an animal model of human brain aging. The student will be involved with experiments that use techniques such as immunocytochemistry, immunofluorescence, confocal microscopy, Western blotting and ELISAs on brain tissue. The student will compare animals treated with antioxidants to those without to see if there is a reduction in brain pathology. Parallel studies involving human brain tissue (Alzheimer's disease and normal aged controls) will also be included as the student acquires the necessary skills.
Contact: Carl Cotman: cwcotman@uci.edu, (949) 824-5847

Karina S. Cramer, Ph.D. Neurobiology
Research is on how specific connections are formed in the brain during development and plasticity. Focus is on the auditory brainstem, where much is known about the connectivity and its importance for brain function. The lab has been examining the role of a family of proteins called the Eph receptors, and their ligands, the ephrins. Lab uses immunohistochemistry, in situ hybridization, and western blots to analyze gene expression. Lab also uses a variety of gene misexpression techniques followed by axon tracing methods to test roles for these proteins. Requirements: completion of Bio110, minimum GPA of 3.0. Must be interested in lab research and motivated to complete an independent project. 3 – 5 units, 10 – 20 hours per week.
Contact: Karina Cramer: cramerk@uci.edu, (949) 824-4211

Steven C. Cramer, Neurology and Anatomy & Neurobiology
RESEARCH DESCRIPTION: This 199 course allows undergraduate students to be part of the research team at the UCI Stroke Center. All activities take place at the Medical Center, in Orange. Students will identify patients admitted for transient ischemic attack and related forms of stroke. Data on such patients will be extracted, reviewed, entered into a database, and later analyzed together. This database serves at least two needs. The first supports maintenance of quality of care at the UCI Stroke Center. The second addresses specific research issues, in particular, understanding the nature of, and optimal response to, transient ischemic attack and related stroke warning syndromes. In addition to above, students are required to (1) participate in a weekly meeting each Wednesday at 3pm, (2) stay for the Stroke Center’s conference each Wednesday at 5pm. REQUIREMENTS TO PARTICIPATE: Attend the above two Wednesday conferences, and review data from patients with stroke and related vascular syndromes. COURSE COMPLETION: Bio 194S GRADE POINT AVERAGE: 3.0 or higher TIME COMMITMENT PER WEEK: minimum of 5 hr/wk, likely higher, plus time traveling to the Medical Center in Orange and back. NUMBER OF UNITS REQUIRED: 3 or more OTHER: Interested students must complete a brief application. Contact Dana Stradling for further information at (714) 456-2343 or dstradli@uci.edu FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Grade based on attendance, participation, and quality of work. OFFICE LOCATION: UCI Med Center, Bldg 53, Rm 203
Contact: Dana Stradling: dstradli@uci.edu, (714) 456-2343 Steven Cramer: scramer@uci.edu, (714) 456-6876

David Cribbs, Neurology & Institute for Brain Aging and Dementia
My research interests are in the degenerative mechanisms that contribute to the onset and progression of Alzheimer’s disease (AD), and the development of novel therapeutic strategies to treat the disease. My laboratory is currently focused on immunotherapy (Aβ-vaccine) as an experimental approach to test the Beta-Amyloid (Aβ) Cascade Hypothesis of AD and as a potential intervention for treating patients suffering from the disease. I utilize transgenic (Tg) mouse models that overexpress human mutant forms of the Amyloid Precursor Protein (APP/Tg). These mice develop extensive AD-like Aβplaque pathology in the brain as they age. Thus, they provide a valuable experimental platform for testing hypotheses on disease mechanisms, as well as potential therapeutic and prophylactic approaches. When the APP/Tg mice are immunized with Aβ peptide, the B cell epitope of Aβ induces antibodies that bind to Aβ, which fafilitates clearance of the peptide from the brain. However, because Aβis a self-antigen it is necessary to break the immune tolerance to self, thus the current immunization protocols rely on very powerful adjuvant systems. Unfortunately, the first clinical trial on AD patients (AN1792) was halted when approximately 6% of the participants developed an adverse inflammatory response in their brains (meningoencephalitis). Speculation on putative culprits for the failure of the clinical trial include the adjuvant (QS21) used to boost the immune response in the elderly patients, and the Aβ “self” T cell epitope. QS21 induced a strongly proinflammatory T cell-mediated (Th1) immune response specific to the Aβ self T cell epitope. Additional factors that may have contributed to the adverse events in the elderly patients that received the vaccine include the level of vascular pathology, changes in blood brain barrier permeability induced by immunization, and the effects of aging on the immune response to Aβ-immunotherapy. My laboratory is developing mouse models that replicate the adverse events that occurred with the AN1792 clinical trial. These mouse models will be used to test the safety of “second generation” vaccines that incorporate alternative strategies for inducing the therapeutic anti- Aβ antibodies without inducing strong Th1 responses. The new vaccine candidates contain “molecular adjuvants” that can amplify the humoral (antibody) response while attenuating the potentially dangerous cell-mediated immune response against Aβ. These include both peptide based and DNA based immunization strategies that include components of the complement system, innate immune system and cytokines in order to enhance antigen presentation and to polarize the immune response toward a Th2 phenotype. New pre-clinical trials in APP/Tg mice are critical for gaining a better understanding of the potential benefits, as well as, the risks associated with Aβ-immunotherapy as a strategy for treating AD patients. Students must be interested in laboratory research and motivated to complete an independent project. Research involves animal handling, behavioral testing, blood and solid tissue collection, fixation and staining of brain tissue and characterization of neuropathology. Students will also participate in vaccinating mice, analyzing serum by ELISA for antibodies against the beta-amyloid peptide. Interested students should submit an updated resume along with a list of completed chemistry, biology and neuroscience courses. Project assignments will be determined by the commitment and enthusiasm of the student for lab work. Interested students are encouraged to visit the lab prior to requesting a position. Minimum requirements: 1) GPA of at least 3.0 2) Minimum commitment 1 year (4 quarters), 2 years preferred. 3) Must commit to work 8-12 hours per week, attend weekly lab meetings and present their research results during lab meetings. In addition, previous lab experience and competence with Photoshop, PowerPoint, Excel and statistical analysis are desirable. The quality of laboratory work, lab meeting presentations and a quarterly paper on research results will figure eqully in the grade evaluation for the 199 students.
Contact: David Cribbs: cribbs@uci.edu, (949) 824-3482

Elysia Davis, Ph.D. Psychiatry & Human Behavior and Pediatrics
RESEARCH DESCRIPTION: We are evaluating the consequences of prenatal exposure to stress and stress hormones for fetal, infant and child development in several NIH funded research protocols. Students working on this project will have the opportunity assist with data collection for several fun and interesting studies of child development. Prior experience working with children is preferred, but not required. We are looking for highly motivated students with an interest in medicine, psychology, biology, or public health, who are willing to work 10-15 hours/week for a minimum of 1 year. Responsibilities will include: administering assessments of child temperament, conducting standardized interviews, evaluating biological and psychosocial indicators of maternal and child stress, and database management. Training will be provided. Students should have access to transportation to and from the Medical Center. REQUIREMENTS TO PARTICIPATE: Transportation to and from the research site, minimum one year commitment, reliable, an interest in working with young children. COURSE COMPLETION: N/A GRADE POINT AVERAGE: N/A TIME COMMITMENT PER WEEK: 10-15 hours per week NUMBER OF UNITS REQUIRED: at least 3 units FACULTY MEANS OF EVALUATION FOR COURSE GRADE: hours completed and quality of work completed. OFFICE LOCATION: 333 City Blvd. West, Orange suite 1200
Contact: Elysia Davis: edavis@uci.edu, (714) 940-1898

Hamid Djalilian, Otolaryngology-Head and Neck Surgery
RESEARCH DESCRIPTION: The purpose of this project is to use a novel method to stimulate the facial, hearing and balance nerves using a penetrating electrode. Restoring hearing, balance, or facial function will be attempted by stimulating the respective nerves in an animal model. REQUIREMENTS TO PARTICIPATE: Life sciences or engineering major or concentration GRADE POINT AVERAGE: 3 or higher TIME COMMITMENT PER WEEK: 8 hrs FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Completion of assigned tasks, feedback on experiments, reading. OFFICE LOCATION: UCI Medical Center Bldg 56, Suite 500
Contact: Hamid Djalilian: hdjalili@uci.edu, (714) 456-5853

Robert Edwards, M.D./Ph.D. Pathology
We study a mouse model of inflammatory bowel disease. The mouse is knocked out of the pertussis-toxin sensitive G-protein alpha subunits, Gia2. These animals spontaneously develop inflammation in the colon after 6 weeks of age which mimics Crohn’s disease; these mice subsequently develop colon cancer. Our work has identified a number of signaling abnormalities whose potential contribution to the development of disease is being studied. We are interested in how the lack of Gia2 affects the development of the immune system, the intestinal epithelium, and how different cell types (epithelial, stromal, and lymphoid) that lack Gia2 interact in regulating mucosal immune responses and the development of cancer in these mice. The work involves mouse husbandry, animal and tissue culture experiments, nucleic acid protein work, and an array of analyte determinations. Completed standard prerequisites for Bio 199, at least a sophomore or greater. Prefer students with expressed interest in aspirations for advanced degrees in biological sciences. GPA 3.3 or greater. Prefer at least a 2 quarter commitment. 8-10 hours/wk, 4-5 hour blocks preferred.
Contact: Robert Edwards: redwards@uci.edu, (949) 824-8576

Gregory, R.D. Evans, M.D., F.A.C.S. Aesthetic and Plastic Surgery Institute
RESEARCH DESCRIPTION: The focus of our lab is tissue engineering. Herein, we work in several different fields: 1. Genetically transfected human embryonic kidney stem cells to produce NGF secretion and to improve peripheral nerve regeneration; 2. Improvement of peripheral nerve regeneration by human adipose derived stem cells differentiated into neural lineage cells; 3. Direct differentiation of human adipose derived stem cells into beta-islet cells; 4. Microcirculation in free flaps, impact of tissue harvest and ischemia reperfusion injury; 5. Improvement of wound healing by enhanced cryoprecipitate; 6. Early detection of vascular thrombosis and disturbances by modulated imaging. Students who are interested will learn about basic techniques in cell cultures, molecular techniques as RT-PCR, ELISA, immunohistology, immunocytology, Western Blot, histomorphometry, and will be involved in animal models including functional evaluations of nerve injuries, etc.. REQUIREMENTS TO PARTICIPATE: 3 quarter minimum commitment, willing to work with rats, some knowledge in medical statistics, experiences in molecular techniques preferred. COURSE COMPLETION: Standard prerequisites for Bio 199 GRADE POINT AVERAGE: 3.5 or greater TIME COMMITMENT PER WEEK: At least 10 hours per week, Blocks of 2-3 hours minimum NUMBER OF UNITS REQUIRED: 3 minimum FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance, quality of work, dedication, empathy. OFFICE LOCATION: 200 S. Manchester Ave., Suite 650, Orange, CA 92868
Contact: Gregory Evans: gevans@uci.edu, (714) 456-5755

Pauline A. Filipek, M.D. Child Neurology
Autism study to prospectively examine the development of infants at high risk for autism (e.g., infant siblings of autistic children) from birth. Goal is to better understand the early-emerging behavioral and biological characteristics that distinguish these infants from those with typical development. Eligibility: highly motivated students interested in behavioral neuroscience research with a GPA greater than 3.4. Must be available between 5-16 hours per week. 3-4 units required. Students will have the opportunity to code videos and learn to recognize the most subtle signs of emerging autism in infant siblings, perform retrospective chart review, review literature, enter data, learn about working with human subjects and the IRB process. Will meet regularly with research staff and PI.
Contact: Sanaz Cherazaie: scheraza@uci.edu

Mark Fisher, M.D. Neurology and Anatomy & Neurobiology
RESEARCH DESCRIPTION: Laboratory of Stroke and Vascular Neurobiology: The laboratory focuses on fundamental mechanisms of stroke, the third leading cause of death in North America. Stroke is usually caused by blood clots blocking arteries of the brain. The laboratory investigates how blood clot formation is regulated by the brain, a process referred to as “brain specific hemostasis regulation”. We use cell culture models of the brain microvasculature (“blood-brain barrier”) and animal models of experimental stroke. REQUIREMENTS TO PARTICIPATE: At least two quarter commitment COURSE COMPLETION: Basic understanding of biological sciences GRADE POINT AVERAGE: 3.0 or higher TIME COMMITMENT PER WEEK: 8 hours minimum NUMBER OF UNITS REQUIRED: 1-4 units FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Oral and written presentations. OFFICE LOCATION: Shanbrom Hall, Room 121, UC Irvine Medical Center
Contact: Mark Fisher: mfisher@uci.edu, (714) 456-6856

Lisa Flanagan, Ph.D. Pathology
Cerebral cortex development and cortical neural stem cell biology Projects with Dr. Flanagan will investigate intrinsic and extrinsic regulators of cortical neural stem cell proliferation, neurogenesis, and neurite outgrowth/regeneration. Projects will utilize cell culture, immunocytochemistry, microscopy, and data analysis. Requirements: Appropriate background in biology with interest in cell biology and neuroscience, good organizational skills, 10+ hours/week, 1+ year commitment, preferred previous lab coursework and GPA 3.0 or higher. Resumes with a completed course list can be sent by email
Contact: Lisa Flanagan: lflanaga@uci.edu
Website: http://www.ucihs.uci.edu/com/pathology/faculty/lflanagan.html

Kirsten Fleming, Ph.D. Psychiatry
Topic: emotion and mental illness. Study of people's reactions to emotional material through subjective ratings, psychophysiological responses and brain imaging. Experience with these patient groups: depression, schizophrenia, Alzheimer's, etc. Some work done at the UCI Brain scanning. Prefers commitment of two years and students interested in neuroscience, mental illness, and brain imaging.
Contact: Kirsten Fleming: kfleming@uci.edu, (714) 456-5936

Donald Forthal, M.D. Medicine
Requirements: GPA 3.5 or better; must be willing to commit at least 15 hours/week and a minimum of 1 year. Description: The laboratory focuses on immune responses to viruses, particularly HIV. We study how antibodies interact with receptors on natural killer cells and macrophages and thereby inhibit viral replication. Students perform a number of techniques including PCR, cell-surface staining, cell culture, and ELISA. Students do not deal directly with HIV. It is hoped that student's can develop their own projects.
Contact: Donald Forthal: dnforthal@uci.edu, (949) 824-3365

John Christian Fox, M.D. Emergency Medicine
Requirements: 3.5 overall GPA, sophomore standing, Friday availability, and two year commitment. Four hours/unit/wk. Minimum of 3 units. Ability to work independently as well as in a group setting. Description: Emergency Ultrasound involves the use of portable ultrasound machines at the bedside of patients in the emergency department. The new field requires clinical research that supports its implementation by proving its safety and efficacy in a wide variety of applications. Currently we have protocols involving pediatric trauma, ocular ultrasound to asses for intracranial pressure, biliary ultrasound, and first trimester complications of pregnancy. 199 students are expected to attend the weekly research meetings held on Friday afternoons in Dr. Fox's office. Also, accuracy of data entry/analysis with an emphasis on clinical application will be tested. Opportunities for publication are available.
Contact: John Christian Fox: jchristianfox@gmail.com, (714) 456-2917

Kathryn Steinhaus French, Pediatrics/Genetics
This project involves obtaining pregnancy outcome information from patients undergoing amniocentesis or CVS procedures at UCI Medical Center. The outcome information is gathered on each perinatologist to determine if patients undergoing these invasive tests have a higher than acceptable risk of pregnancy complications. Students also assist in data entry and are given the opportunity to participate in patient conferences as well as observe genetic counseling and prenatal testing procedures such as ultrasound, amniocentesis and CVS at UCI Medical Center REQUIREMENTS TO PARTICIPATE: 3 quarter minimum commitment COURSE COMPLETION: Open GRADE POINT AVERAGE: Open TIME COMMITMENT PER WEEK: 4-8 hours per week NUMBER OF UNITS REQUIRED: 2 or 4 OTHER: Students interested in Genetic Counseling, obstetrics, genetics and/or obstetrics are encouraged to participate FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Students are graded based on ATTENDANCE, AS WELL AS accurate and timely completion of duties performed in a professional manner
Contact: Kathryn Steinhaus French: kasteinh@uci.edu, (714) 456-6883

Ron D. Frostig, Ph.D. Neurobiology and Behavior
RESEARCH DESCRIPTION: The focus of our lab is cortical plasticity, which is the ability of the cerebral cortex to continuously modify itself, and therefore is fundamental for understanding higher brain functions including learning and memory. We use functional imaging, electrophysiological recordings, anatomical and behavioral techniques to study plasticity and its underlying mechanisms. Our research interests include studying naïve rats as well as rats whose life experience has changed in some way, such as in response to living in a more ‘naturalistic habitat’ rather than a laboratory cage or in response to injury such as a stroke. See our website for more details on our lab’s research. Project assignments will be determined based on the student’s commitment and enthusiasm and include data analysis, anatomy, assisting with imaging experiments, and/or establishment/maintenance of the naturalistic habitats. REQUIREMENTS TO PARTICIPATE: Minimum 1 year commitment; minimum 2nd year student; willingness to work with rats/mice. GRADE POINT AVERAGE: minimum 3.3 GPA TIME COMMITMENT PER WEEK: minimum 6 hours/week NUMBER OF UNITS REQUIRED: minimum 2 units FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance; productivity; quality of work; enthusiasm. OFFICE LOCATION: 2219 McGaugh Hall
Contact: Ron Frostig: rfrostig@uci.edu, (949) 824-5031
Website: http://frostiglab.bio.uci.edu/

John Fruehauf, M.D./Ph.D. Oncology
Description: Students will carry out in vitro cell line work to determine the effects of anticancer agents on tumor cell and tumor vascular cell growth. Antiangiogenesis models will be emphasized. Cancer pharmacology will be reviewed. For some studies, biomarker correlates with drug response will be determined using immunohistochemistry, image analysis, flow cytometry, gene expression arrays and proteomics techniques. Students will be expected to define a hypothesis driven project, present a15 minute lecture summarizing their objectives, prepare a mid term oral and written report, and present a final oral and written report. When data are of adequate quality, the student will be encouraged to submit a manuscript for publication and to present their work at the UCI science fair. Prerequisites: Introductory Biology and Chemistry; Bio 194; Cum GPA>3.0. Organic Chemistry and Biochemistry preferred.
Contact: John Fruehauf: jfruehau@uci.edu, (714) 456-5153

Pietro Galassetti, M.D., Ph.D. Pediatrics, General Clinical Research Center
RESEARCH DESCRIPTION: Our lab currently has 4 human research protocols funded by the NIH and/or JDRF. 1) We are researching the adaptive responses to exercise in Type 1 Diabetic children/adolescents with hypoglycemia and/or hyperglycemia. Cytokines such as (IL-4, IL-6, IL-1α, IL-1β, TNF-α, ), growth factors (IGF-I, IGF BP-1), and neuroendocrine components (Glucagon, Cortisol, GH, Epi, Nor-Epi) are measured via ELISA and LUMINEX assay techniques. 2) The second protocol is aimed at defining the inflammatory and oxidative stress responses during exercise in children with obesity/metabolic syndrome, and in healthy controls, in fasting conditions and after ingestion of a high-fat meal. Data will be analyzed attempting to ascertain the effects of maturational status, gender, and physical fitness. In addition to the assays listed above, a broader panel of oxidative markers will be used, including measurement of leukocyte gene expression. Assessment of total and regional body composition by DEXA, and assessment of the cardio-respiratory responses to exercise will also be performed. 3) The third protocol is a joint effort with the Blake/Rowland lab in the Department of Chemistry to define and identify correlations between exhaled breath gas profiles, plasma glucose, insulin, FFAs, ketones and oxidative stress markers in healthy and Type 1 Diabetic adults, in an effort that may lead to the creation of new techniques allowing the accurate, non-invasive measurements of these variables. In addition to the assays listed above, a sophisticated gas chromatography/mass spectrometry analysis of expired gases is performed. 4) The fourth protocol researches the effects of exercise on the immune system’s responses in adult males. Strenuous exercise is used to stimulate cellular and humoral changes through recruitment and activation of blood leukocyte subpopulations. For key immuno-modulatory mediators, in addition to systemic circulating concentrations, also levels of gene expression and intracellular concentrations in leukocyte subpopulations will be studied. REQUIREMENTS TO PARTICIPATE: Students interested in clinical research. Enthusiastic, responsible individual who demonstrates a willingness to learn. Requires working an occasional weekend day (approximately 1-2 days per month). Good organizational and time management skills are crucial. Students should have access to transportation to and from the Medical Center. A commitment of at least two consecutive quarters is necessary. COURSE COMPLETION: Completion of standard prerequisites for 199. GRADE POINT AVERAGE: Minimum 2.5 GPA TIME COMMITMENT PER WEEK: 8-12 hours/week NUMBER OF UNITS REQUIRED: 2-3 OTHER: Students will have the opportunity to learn about working with human subjects in clinical research environment. Review of ongoing research projects (grants) and UCI regulatory documents (IRB, GCRC). Under supervision, the student will assist with (including but not limited to) plasma analysis on Beckman Glucose Analyzer, recording and entering of data in existing databases relating to research studies, and review of pertinent literature. Structure and organize data into presentation formats for national and local meetings, journal club meetings and scientific manuscript submissions. Will meet weekly with PI, staff, and participate in department and laboratory meetings. Training will be provided. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Attendance, quality of work and performance of assigned tasks, participation in laboratory meetings, and acquired knowledge of research field. OFFICE LOCATION: 1111 Hewitt Hall. If interested, please contact Rebecca Flores (rlflores@uci.edu, 949-824-4433) and cc Dr. Galassetti (pgalasse@uci.edu) on your correspondence."
Contact: Rebecca Flores: rlflores@uci.edu, (949) 824-3353

Anand Ganesan, MD.,Ph.D. Dermatology
RESEARCH DESCRIPTION: Our research utilizes novel systems-level approaches to identify and characterize the genes that govern melanin pigmentation and the genes that control melanoma chemoresistance. The ultimate goal of these studies is to identify novel agents to treat melanoma and disorders of pigmentation. REQUIREMENTS TO PARTICIPATE: minimum sophomore standing COURSE COMPLETION: must have completed 100L, 114L, or 116L GRADE POINT AVERAGE: 3.5 TIME COMMITMENT PER WEEK: 16- 20 hours NUMBER OF UNITS REQUIRED: 3-4 units FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Evaluation will be based on ability to utilize the scientific method to design and critically evaluate the results of experiments performed in the laboratory.OFFICE LOCATION: 310A Med Surge II
Contact: Anand Ganesan: aganesan@uci.edu, (949) 824-2926

David Gardiner, Dev & Cell Biology
Role of signaling pathways in the induction of scar-free wound healing and limb regeneration. Successful completion of first year courses – strong interest in regeneration. GPA 3.0. Minimum of 10 hours/wk. Typically 4 units. *Note: Enroll in course code under Dr. Susan Bryant.
Contact: David Gardiner: dmgardin@uci.edu, (949) 824-2792

Brandon Gaut, Ph.D. Ecology & Evo

Contact: Brandon Gaut: bgaut@uci.edu, (949) 824-2564

Jean Gehricke, Ph.D. Psychiatry and Human Behavior
RESEARCH DESCRIPTION: Dr. Gehricke’s research focuses on why individuals with ADHD have higher smoking rates and lower cessation rates compared to the general population. Learn about the effects of nicotine and cigarette smoke on human behavior and brain circuitry as well as the genetic and environmental risk factors that lead to smoking initiation and nicotine addiction. Students will be trained in implementing study protocols, data collection techniques, data entry, and analysis. Good opportunity for students interested in careers in medicine, clinical psychology, and drug abuse treatment and prevention. REQUIREMENTS TO PARTICIPATE: Minimum commitment of one year TIME COMMITMENT PER WEEK: 8-10 hours/ week NUMBER OF UNITS REQUIRED: 3-4 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Reliability and consistency in attendance. Completion of assignments and required hours. Discussions of research papers. Brief paper in one area of drug abuse research. OFFICE LOCATION: 19722 MacArthur Blvd., Irvine
Contact: Nuong Hong: nhong@uci.edu, (949) 824-8216

Paul Gershon, Ph.D. Molecular Biology & Biochemistry
I am active in four areas: (1) We operate a protein (roteomics? mass spectrometry facility containing two powerful, state-of-the-art tandem mass spectrometers along with nanoflow/nanocapillary two-dimensional peptide fractionation HPLC with robotics. Last year, several 199s deftly learned to operate these instruments and used them to, among other things, identify all the proteins in some of the world largest viruses. We will now be identifying and quantitating the proteins characteristic of diseased/tumorigenic cells and developmental abnormalities. (2) My lab is currently funded to investigate the structure and molecular dynamics of the enzyme poly(A) polymerase (PAP), using the vaccinia virus enzyme as a model. The vaccinia PAP was the first PAP for which a gene was identified (by the P.I.), and we recently learned its three-dimensional structure. Vaccinia PAP is the only known polymerase that can translocate independently on single-stranded nucleic acid. How does it do this on a non-rigid polymer? Come to my lab and solve the mystery. (3) A collaboration between my lab and that of Dr. Alex McPherson (MB&B) has led to the clearest images obtained thus far of vaccinia virus particles and subviral assemblies, providing a unique opportunity to identify viral proteins and functions present in various subviral assemblies. This dovetails well with protein mass spectrometry (above). (4) I am also funded to investigate the catalytic mechanism of RNA O-methylation. Vaccinia virus protein VP39 provided the first three-dimensional structure for any poxvirus protein and any RNA methyltransferase. The structure was then solved again, with bound RNA substrate and cofactor. Using various chemical, biochemical and NMR techniques, we are elucidating aspects of its catalytic mechanism from hypotheses arising from the three-dimensional structure. Overall, prior undergraduate interns in my lab have been included as authors on manuscripts submitted for publication, and some current ones will be.
Contact: Paul Gershon: pgershon@uci.edu, (949) 824-9606, 7954

Laura Glynn, Ph.D. Psychiatry
Students will work on research studies involving the role of biological and psychological processes in pregnancy and on prenatal factors influencing later psychiatric health. The research protocols are varied and students will participate in EEG, archival chart review and data analysis. Students who are willing to make a three quarter commitment are preferred. Training will be provided.
Contact: Laura Glynn: lglynn@uci.edu, (714) 940-1925

Sastry Gollapudi, Ph.D. School of Medicine
RESEARCH DESCRIPTION: Our research focuses on the role of Toll Like receptors (TLRs) in Breast cancer cell survival. These receptors are pattern recognition receptors. Pattern recognition receptors are expressed on a wide variety of cells and they promote phagocytosis. TLRs signal via conserved pathways to induce [1] secretion of a number of proinflammatory cytokines, [2] apoptosis, and [3] potentiate apoptosis induced by anti-cancer agents. The expression of TLRs in breast cancer cells is suggested by the capacity of breast cancer cells to phagocytose yeast (which is recognized by TLR 2 and dectin, a PRR that detects beta glucan of yeast cell wall) and induction of apoptosis in breast cancer cells by yeast cell wall components. Students will gain experience in a range of research techniques including cancer cell biology , molecular biology and Biochemistry REQUIREMENTS TO PARTICIPATE: Students beginning in summer and continuing through the year will be given preference COURSE COMPLETION: Basic course work in Biology and Biochemistry GRADE POINT AVERAGE:3.0 and above TIME COMMITMENT PER WEEK: 16-24 hours /week NUMBER OF UNITS REQUIRED: 4 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: The quality of laboratory work and presentation of their research findings, both written and oral, in the lab meetings will figure eqully in the grade evaluation. OFFICE LOCATION: Room C348, MedSCi Bld 1
Contact: Sastry Gollapudi: svgollap@uci.edu, (949) 559-5820

Celia Goulding, Ph.D. Molecular Biology& Biochemistry
My research is based on structure-function determination of Mycobacterium tuberculosis proteins in the hope of finding novel anti-TB drug targets. The techniques that we utilize include X-ray crystallography, biochemical characterization and mass spectrometry. My main focus is on a mycobacterial iron up-take system that scavenges iron from humans in the form of heme, and then uptakes this heme to be broken down for iron usage either within the bacterial membrane or cytosol The student research projects may include cloning, protein expression and purification and crystallization trials initially. There will also be some protein-protein interaction experiments to carry out. Completion of Bio 194S (biosafety and ethics) is required and a basic course in biochemistry/chemistry would be preferable. Must be highly motivated and be prepared to work12 hours/wk. 3 units. GPA 3.2 or better.
Contact: Celia Goulding: celia.goulding@uci.edu, (949) 824-0337

Sudhir Gupta, M.B./B.S./Ph.D. Medicine
Two positions available. One would work on molecular biology technology involved in dissecting some novel gene from two infants with unique congenital immunodeficiency and other for molecular mechanisms of cell death. Should have minimum GPA of 3.8 who are majoring in Biology.
Contact: Sudhir Gupta: sgupta@uci.edu, (949) 824-5818

Brad Hawkins, Ph.D. Ecology
Project involves a study of the global pattern of species diversity for birds. Students must be willing to work at least 6 hours per week (3 hours per unit). Students who work on project a second year are expected to conduct a small independent study related to the major project.
Contact: Brad Hawkins: bhawkins@uci.edu, (949) 824-5384

Elizabeth Head, Ph.D. Neurology
Assist in neurobiological studies of human aging. Down syndrome and Alzheimer disease. The project involves studying the brains of adults with Down syndrome in comparison to age-matched controls and Alzheimer’s disease tissues. Students will learn techniques such as immunocytochemistry, immunofluorescence, confocal microscopy, Western blotting and enzyme linked immunosorbant assays on brain tissue samples. Quantitative anatomical techniques will also be systematically used. Requirements to participate: Background in neurobiology and an interest in human brain aging and disease. Students must be enthusiastic and reliable and preference will be given to those that can make a multi-quarter commitment (at least 1 year). Students must all meet the requirements for working with human tissue as indicated by the program outline. Interested students must be able to work in the laboratory for at least 4 hours per day on 2 consecutive days in order to be able to complete experiments. 4 units required. 12 hours/wk. Minimum 3.0 GPA.
Contact: Elizabeth Head: ehead@uci.edu, (949) 824-8700

Bang H. Hoang, M.D. Orthopaedic Surgery
RESEARCH DESCRIPTION: Orthopaedic Oncology Lab focusing in molecular biology and genetics of bone and soft tissue tumors. Techniques include DNA and protein gel electrophoresis, tissue culture, gene transfection, microscopy, biological assays, immunohistochemistry. REQUIREMENTS TO PARTICIPATE: complete Clinical package to work at UCIMC laboratory. COURSE COMPLETION: Bio. 194 Safety & Ethics GRADE POINT AVERAGE: 3.3 or above TIME COMMITMENT PER WEEK: 3-4 hours per week for each credit NUMBER OF UNITS REQUIRED: 1-5 credits FACULTY MEANS OF EVALUATION FOR COURSE GRADE: completion of research proposal, attendance, presentation at lab meeting, attending monthly lab meetings, reading journal articles. OFFICE LOCATION: UCIMC, Pavillion 3, second floor.
Contact: Bang Hoang: bhhoang@uci.edu, (714) 456-7752

Franz Hoffmann, Ph.D. Development & Cell Bio

Contact: Franz Hoffmann: fjhoffma@uci.edu, (949) 824-4346

Randall F. Holcombe, M.D. Hematology/Oncology
RESEARCH DESCRIPTION: Students will be involved in research investigating the role of Wnt signaling pathway in human cancer. A primary area of focus is colon carcinogenesis. Studies investigating the activity of a natural compound, resveratrol, on the Wnt pathway are also ongoing in the laboratory. Students will work under the direction of Dr. Holcombe and in collaboration with several post-doctoral fellows and research associates in the laboratory. Molecular and cell biology techniques will be utilized in this research. REQUIREMENTS TO PARTICIPATE: Ability to travel to the Cancer Center in Orange where the laboratory is located. GRADE POINT AVERAGE: 3.0 TIME COMMITMENT PER WEEK: 10-12 hours FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Supervision of progress on assigned projects, written reports, presentations to laboratory group and abstract preparation. OFFICE LOCATION: UCIMC, Building 56, Rm 247
Contact: Randall Holcombe: rholcomb@uci.edu, (714) 456-5153

Todd Holmes, Ph.D. Physiology and Biophysics
RESEARCH DESCRIPTION: Circadian Rhythms REQUIREMENTS TO PARTICIPATE: Student interview COURSE COMPLETION: BIO 194 GRADE POINT AVERAGE: 3.0 TIME COMMITMENT PER WEEK: 10 hour minimum NUMBER OF UNITS REQUIRED: 3 - 4 (3 minimum) FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Student presentation and Lab meeting attendance. OFFICE LOCATION: D340 Medical Science I, Irvine, CA 92697-4560
Contact: Todd Holmes: tholmes@uci.edu, (949) 824-0006

Lan Huang, Ph.D. Physiology, Biophysics
Our research focuses on developing and employing mass spectrometry-based proteomic approaches to answer a series of biological questions related to cancer diagnostics and treatment. Current projects include characterization of proteasome complex composition, their post-translational modifications and mapping protein interacting network in normal and cancer cells; investigation of protein ubiquitination and ubiquitin-like protein modifications to understand their functions in turmorigenesis. Requirements: completion of organic chemistry, cell biology, biochemistry. GPA 3.0 or higher, one year commitment.
Contact: Lan Huang: lanhuang@uci.edu, (949) 824-8548

David Imagawa, M.D., Ph.D., FACS Hepatobiliary and Pancreas Surgery
RESEARCH DESCRIPTION: The Division of Hepatobiliary and Pancreas Surgery involves examination of various conditions and treatments affecting diseases of the liver, pancreas, and biliary tree. Students will be expected to attend weekly clinics on Monday mornings and Thursday afternoons, as well as work independently in the office. Student tasks will include maintaining the research database, statistical analysis, and other research duties as necessary. Ability to learn new applications. Experience with Excel, Access, SPSS, Adobe Premiere, Adobe Photoshop, Adobe Illustrator preferred. Must be willing to travel to medical center. Student will be supervised by the PI and the surgical research resident. REQUIREMENTS TO PARTICIPATE: 3.0 overall GPA and sophomore standing COURSE COMPLETION: Bio 194S. Statistics and physiology recommended but not required GRADE POINT AVERAGE: 3.0 TIME COMMITMENT PER WEEK: Approximately 12 hours/week NUMBER OF UNITS REQUIRED: 4 FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Quality of work, fulfillment of time commitment OFFICE LOCATION: 101 The City Drive South Bldg 56 Room 202 Orange, CA 92868
Contact: Maki Yamamoto: myamamot@uci.edu, (714) 456-3885

Mahtab Jafari, Pharm.D. Pharmaceutical Sciences
The use of animal models for screening and evaluating anti-aging pharmaceutical and botanical compounds is a promising approach for drug discovery. While testing anti-aging compounds using the premier animal genetic systems, Drosophila melanogaster and Caenorhabditis elegans, has already started, the fundamental biological issues involved in such screening have not been systematically formulated. As a result, after selecting potential anti-aging compounds to be tested, we need appropriate methodologies to study the pharmacology of aging in model species. Once these methodologies or rules of investigation are developed, we may consider extrapolating these experimental findings with such systems to the treatment of human aging. However, there are a number of potential artifacts, confounds, and errors that can arise in such research programs. In order to minimize these problems, I developed the following assays as the rules of investigation in anti-aging pharmacology and am in the process of applying them to a number of potential anti-aging compounds. These are the rules of investigation that I propose: Since human adulthood is almost exclusively a period of aging, data that conflate aging and late life should not be extrapolated to human aging. 2) The response to candidate medications should show a normal drug-dose response pattern, although not necessarily a linear response. 3) Medicated animal models should not be hypometabolic. 4) Medicated animal models should not show pronounced reductions in fertility. 5) Medicated animal models should not exhibit general nervous system depression. 6) The effect of the medication should not be highly sensitive to the culture environment. 7) The effect of the medication should not be highly dependent on the genetic ancestry of the stock employed, leaving aside inbreeding, which should be avoided because humans are not generally inbred. While these rules do not guarantee successful extrapolation of successful drug results from the animal model to humans in a clinical setting, the failure to adhere to these rules should raise doubts about such extrapolation. In my research, I attempt to address this task. Interest in a career in biomedical research is required. All students start as Bio. 198, group research students. Progress to Bio. 199 status depends on the student's ability to work independently. NO student begins in the Bio. 199 course. 3-4 units required involving 10-12 hours/week. A 2-year commitment and a desire to apply for a UROP grant, SURP fellowship, and Excellence in Research is recommended. Must be highly motivated to complete an independent project while working closely with the research staff and PI. Biology and Chemistry Majors preferred with a minimum 3.3 GPA.
Contact: Mahtab Jafari: mjafari@uci.edu, (949) 824-0145
Website: http://www.faculty.uci.edu/profile.cfm?faculty_id=5313

James Jakowatz, M.D. Surgery
RESEARCH DESCRIPTION: Examination of, and maintenance of, melanoma center patient data base; outcome of research: accuracy, failure, recurrence, survival and complications in sentinel lymph node mapping. REQUIREMENTS TO PARTICIPATE: only BID 194 COURSE COMPLETION: per semester / 3-5 page paper GRADE POINT AVERAGE: 3.0 TIME COMMITMENT PER WEEK: 3–4 hours/week NUMBER OF UNITS REQUIRED: 3–4 OTHER: N/A FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Accuracy of data entry / chart review success in focus on a specific clinical outcome. Example: # of patients with lymphocele or lymphodema; or, false negative lymph node basin rate / recurrence in a node basin originally staged clear. OFFICE LOCATION: UCIMC Bldg 56
Contact: James Jakowatz: jgjakowa@uci.edu, (714) 456-8171

Sunny Jiang, Ecology and Revolutionary Biology, Civil and Environmental Engineering
Research in Dr. Jiang's lab focuses on the following three areas: 1) aquatic/marine microbial ecology, 2) water quality microbiology, 3) epidemiology of exposure to recreational waters. Her group uses molecular method to uncover the genomes and interaction of organisms in the environment. Over the past decade, her team has developed rapid and sensitive methods for detection and quantification of human virus contamination in aquatic samples. Their results have suggested that current coastal water standards do not adequately reflect the viral quality of recreational waters. Working together with environmental engineers, coastal physical oceanographers and remote sensing scientists, they have recently discovered that surf zone water quality is impacted by multiple factors including global climate changes, land use patterns and human activities. Working directly with the City of Newport Beach and Regional Water Quality Control Board, they have investigated the relationship between fecal indicator bacteria and human viruses in Newport Bay. The results showed that the occurrence of these two types of organisms is uncoupled largely due to the differences in the source and survival of each type. Another area of her research addresses human health risk from exposure to coastal waters. Her research group is currently developing web-based survey tools to understand the relationship between human health risk and coastal water quality. Sample projects in her lab include investigation the causes and decline of harmful algal blooms (red tides), detection of pathogens in water, epidemiological surveillance of recreational illnesses. REQUIREMENTS: Students should be prepared to develop independent projects and spend at least 10 hours per week on research. A multi-quarter commitment is strongly preferred, and students will be expected to participate in lab activities and meetings. MEANS OF EVALUATION: grades will be based on time committed to the project and the quality of research.
Contact: Sunny Jiang: sjiang@uci.edu, (949) 824-5527

Joie Jones, Ph.D. Radiological Sciences
RESEARCH DESCRIPTION: Medical imaging methods provide a unique tool for the quantitative evaluation of the healing process, even when brought about by non-conventional means. Our ongoing research is investigating subtle energy medicine using contemporary medical imaging and laboratory methods. Here “subtle energy” refers to a set of modalities, such as acupuncture and Pranic Healing, in which the mechanisms of action are not well understood. Students would have the opportunity to participate in various aspects of this ongoing work depending on their background and interest. Activities range from library/internet searches through the statistical analysis and evaluation of large data sets. REQUIREMENTS TO PARTICIPATE: Depends on specific project undertaken. Several projects require a strong background in mathematics and physics. COURSE COMPLETION: Depends on specific project undertaken. GRADE POINT AVERAGE: 3.5 although exceptions may be made. TIME COMMITMENT PER WEEK: 9 to 12 hours per week. NUMBER OF UNITS REQUIRED: 3 to 4 units FACULTY MEANS OF EVALUATION FOR COURSE GRADE: (1) active participation in regular meetings and (2) on-time completion of assigned tasks (which may involve a written report).
Contact: Joie Jones: jpjones@uci.edu, (949) 824-6147

Hans Keirstead, Ph.D. Anatomy, Neurobiology
Three positions available: 3 different projects. Students must be highly motivated. • will be exposed to animal (in vivo) experiments for an average of 10-15 hours per week. Must be willing to work on weekends on occasion. 90% animal work. Assist in various surgical procedures such as spinal cord contusion injuries, and intra spinal cellular transplantations. Post surgical care will be monitored by way of antibiotic and saline injections as needed. Daily injections of immunosuppressant will also be administered to all the animals. Some behavioral work may also be performed throughout the experiments. There will also be opportunities to be trained in technical surgical procedures such as laminectomy and dorsal hemi-sections. Previous course work in neurobiology/biology field is highly desirable. (2) Project includes treatment on rat spinal cord and spinal cord extraction, cutting and immunological analysis. Experience in the following areas would be appreciated but not necessary: cryostat cutting and immunological staining, previous animal handling experience or willingness to work with live animals, and previous academic course work in the neurobiology/biology field. Student will also have the opportunities to learn more accurate techniques such as spinal cord extraction and laminectomy under the supervision of staff members. Supervisor is a post doctoral fellow. Work an average of flexible 10 – 15 hours per week. (3) Research will include, assisting in surgery, cryosectioning, animal care, histology and immunohistochemistry. Must have flexible hours and be willing to work 10-15 hours per week. Must be highly motivated and interested in neurosciences, particularly spinal cord injury.
Contact: David Ferguson: dferguso@uci.edu, (949) 824-3265

Kristen Kelly, M.D. Dermatology
Optimizing current treatments and developing alternative therapies for port wine stain birthmarks (PWS). The current treatment of choice for PWS is the pulsed dye laser (PDL). However, multiple treatments are required and less than 15% of lesions are cleared completely. We are developing ways to improve current treatments including non-invasive imaging of blood vessels and the use of anti-angiogenic factors. We are also exploring novel treatment options including photodynamic therapy. Preliminary evaluations are done on a rodent dorsal skinfold model and then promising approaches are further evaluated in clinical trials. COURSE COMPLETION: Preferably beginning engineering and/or biology courses. GRADE POINT AVERAGE: No requirement TIME COMMITMENT PER WEEK: 10-20 hours/week NUMBER OF UNITS REQUIRED: No requirement FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Performance during the project – specifically involvement in data collection and manuscript preparation.
Contact: Kristen Kelly: kmkelly@uci.edu, 949-824-7997

Virginia Kimonis, Pediatrics
RESEARCH DESCRIPTION: Dr. Virginia Kimonis is the new chief of the Division of Genetics and Metabolism in the Department of Pediatrics at UCI. While involved in many aspects of clinical genetics and clinical research (multicenter clinical NIH studies involving natural history and genotype- phenotype studies in Prader-Willi and Craniosynostosis syndromes), she has an international reputation for her clinical and laboratory research work in inherited muscle diseases that occur in combination with diseases of bone and/or dementia (also known as IBMPFD) Her group localized the gene for the disorder to chromosome 9, identified the causal gene as VCP (CDC48 or p97) and are now identifying the key pathways and functions that are disrupted by the mutations they have found in the affected families. They are also developing a knock-in animal model of the disorder and are working on developing better clinical evaluations, diagnostic testing and treatments for families with IBMPFD, but also for those with other sporadic and hereditary diseases that share components of IBMPFD. OFFICE LOCATION: Hewitt Hall, Irvine and Tower Bldg, Orange
Contact: Virginia Kimonis: vkimonis@uci.edu, (714) 456-5791 or (949) 824-0571

John Krolewski, MD- PhD Pathology
Our lab is investigating mechanisms of cytokine signaling in both normal and cancerous cells. The experimental systems include both cells in culture and rodent models. In one set of experiments we are investigating an unusual signaling mechanism involving proteolytic cleavage (proteolysis) within the plasma membrane. Another project involves the determining how the natural product Madindoline acts to block IL6 signaling. IL6 is a growth factor for some tumors, including prostate cancers. Finally, in a third project, we are investigating the mechanism of androgen withdrawal induced cell death in prostate epithelial cells. The same mechanisms may be involved in the death of prostate cancer cells. Students will be assigned individual projects and will work under the mentorship of Dr. Krolewski and/or other personnel in the lab. A minimum of a 15 hr/wk commitment is required, for at least 6 quarters. Sophomores interested in making a long term commitment are preferred.
Contact: John Krolewski: jkrolews@uci.edu, (949) 824-4089

Young Jik Kwon, Ph.D. Pharm Sci; Chem Engnring & Materials Sci
RESEARCH DESCRIPTION: Timely, complete, and convenient treatment of many diseases is not yet within reach, although many potent therapeutics have been identified and developed. Successful delivery of therapeutics depends on finding paths toward ideal forms of treatment. In BioTherapeutics Engineering Laboratory (BioTEL) in departments of Pharmaceutical Sciences, and Chemical Engineering and Materials Science tackles this problem by identifying the limitations of current therapeutics and the reasons for those limitations. Target therapeutics are then selected and conjugated in the form of micro- and nanoparticles, using organic chemistry and carrier fabrication tools. BioTEL is currently working to develop drug delivery systems that selectively localize therapeutics in the body, down to the level of cellular compartments, and offer controlled release of therapeutics so that drugs can be delivered only at the desired time. BioTEL employs techniques to immobilize therapeutics, including cancer chemotherapy agents, imaging probes, therapeutic genes and proteins, and cells. Once carriers are localized in a target site through passive and active targeting mechanisms, therapeutics can be triggered to be active by pathological signals. Synthesis of novel carrier materials, innovative fabrication of carriers, elucidation of extracellular and intracellular behaviors of drug/carriers, and development of new drug release mechanisms are the key scientific emphases in BioTEL. Opportunities for undergraduate researchers including nanocarrier synthesis and characterization as well as in vitro and in vivo tests, depending on needs in the lab and qualification of participants. REQUIREMENTS TO PARTICIPATE: Basic molecular biology and organic chemistry COURSE COMPLETION: Sophomore and juniors are ideal but seniors with previous experience will also be considered. GRADE POINT AVERAGE: 3.0 or higher TIME COMMITMENT PER WEEK: Minimum 10 hrs spread through weekdays and weekends NUMBER OF UNITS REQUIRED: 3 or more OTHER: Students with intention of a long-term participation will be given high priority. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Written reports, literature review, presentation in the group meeting, evaluations from graduate co-workers. OFFICE LOCATION: Engineering Tower 944F
Contact: Young Jik Kwon: kwonyj@uci.edu, (949) 824-8714

Arthur D. Lander, Ph.D. Dev. & Cell
Role of proteoglycans in mouse brain development. This research is to study the development of the mammalian brain by analyzing mutant mice. The lab has made mutants that lack a proteoglycan, glypican – 1, and which are deficient in brain growth. The goal is to determine what cellular signals are disrupted by this mutation. Methods will include tissue histology, molecular biology, and mouse genetics. Learning how proteoglycans affect brain development in mice will ultimately contribute to the understanding of human birth defects and diseases. Requirements: minimum of 10 hours per week, has a GPA of at least 3.4, preferably has junior standing (but will consider sophomores with excellent grades), and is interested in continuation of this project in future quarters.
Contact: Arthur Lander: adlander@uci.edu, (949) 824-1721
Website: http://lander-office.bio.uci.edu/landerfacts.html

Michael Lilly, Medicine
RESEARCH DESCRIPTION: We are developing new diagnostic tests that can identify subjects with prostate cancer before they develop clinically obvious disease. Saliva and plasma collected from normal males (no evidence of prostate disease) and from men having prostate cancer will be used for isolation of RNA. RNA will be subjected to RT-PCR, qPCR, and microarray analysis in an attempt to identify and compare types of RNA molecules (derived from different genes) in the saliva and plasma from men with or without prostate cancer. Identification of unique kinds of RNA molecules in the blood or saliva of prostate cancer subjects may allow us to develop diagnostic tests to identify prostate cancer without needing a biopsy. REQUIREMENTS TO PARTICIPATE: Completion of Bio. 194 Safety & Ethics course prior to enrollment. General background in biology/biochemistry/molecular biology and/or oncology. Motivation and interest in research. Prior lab experience required. Knowledge and experience in molecular biology techniques preferred. COURSE COMPLETION: GRADE POINT AVERAGE: 3.0 + TIME COMMITMENT PER WEEK: 10-12 hrs NUMBER OF UNITS REQUIRED: 2-3 units (4hrs/unit) OTHER: Students will be involved not only in generating laboratory data, but also in literature reviews and laboratory presentations. Substantial contribution may lead to paper co-authorship. FACULTY MEANS OF EVALUATION FOR COURSE GRADE: Commitment and enthusiasm in finishing assignments and written reports. OFFICE LOCATION: Bldg 56 Rm 248 Rt 81
Contact: Michael Lilly: mlilly@uci.edu, (714) 456-5153

John Longhurst, M.D./Ph.D. Cardiology
Investigates the neurophysiological mechanisms involved in autonomic reflexes including cardiovascular and acupuncture related mechanisms. Also studying the role of mechanical factors and metabolic products such as bradykinin in stimulating ischemically and reperfusion-sensitive cardiac sensory nerves. These nerves are responsible for angina and a number of other reflexes that lead to hypertension in the immediate post-bypass period, arrhythmias, hypotension, nausea and vomiting. Upper division students who have had introductory physiology/neurophysiology course; 3 quarter commitment. However, year 2 Bio. Majors considered. Students will be involved in whole-animal surgical procedures and histological procedures prefer students who can commit to at least 2-3 hour time blocks. At lest 6 hours/wk. GPA 3.0.
Contact: Stephanie Tjen-A-Looi: stjenalo@uci.edu, (949) 824-5602

Shahram Lotfipour, M.D.,M.P.H. Emergency Medicine Emergency Medicine
Emergency Medicine Research Associates Program (EMRAP)- The EMRAP program created in 1997 by Dr. Federico Vaca is focused on facilitating clinical research in the Department of Emergency Medicine (EM) at the University of California Irvine Medical Center (UCIMC) in Orange. EMRAP provides undergraduate students first hand exposure to clinical research in the active Level 1 Trauma Center Emergency Department. They will learn to enroll patients in clinical research as well as become very familiar with research methodology. In addition to research exposure, students receive weekly clinical case and topic discussions as well as quarterly procedure workshops. The unique blend of first-hand clinical experience and scientific research has given EMRAP alumni a unique advantage when applying to medical (allopathic and osteopathic), dental, pharmacy, physician assistant and public health programs around the country. While participating in EMRAP, research associates have an extensive opportunity to interface, inform and educate, and dialogue with patients regarding any one of a number of excit